Biosynthesis and degradation of juvenile hormone in corpora allata and imaginal wing discs of Galleria mellonella (L.)

Wiśniewski, Jacek R.; Muszyńska-Pytel, M.; Grzelak, Krystyna; Kochman, Marian

doi:10.1016/0020-1790(87)90167-3

Cited by 24 publications

(11 citation statements)

References 30 publications

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“…A comparison of the rate of JH biosynthesis with that of JH degradation revealed that in Galleria JH hydrolysis in vitro was somewhat higher than synthesis after the second day of the last larval instar [11]. In Nauphoetu the rate of JH biosynthesis in vitro on days 9-10 was -10-20 pmol/CC-CA/h ( Table 4) and that of (10R)-JH I11 degradation in vitro was -800 pmol/CC-CA/h (Fig.…”

Section: Jh Degradation By Cc-camentioning

confidence: 99%

“…At certain developmental stages corpora allata of Munducu sexta have been shown in vitro to release JH acid, which appears to be methylated to JH by target tissues [7-91. In this species, as well as in Gulleriu mellonella, CA have been reported to degrade JH, JH acid being the major metabolite [7,10,11].…”

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confidence: 90%

“…Most of the work on JH degradation has been concentrated on the hemolymph [13], and only very little information is available concerning JH degradation in CC-CA. To our knowledge only data on CC-CA and CA of larvae from two lepidopterous species, Munducu sextu [7] and Gulleriu rnellonellu [10,11], have been reported. In the latter the highest rate of degradation of racemic JH I was 4 pmoVCC-CA/h [lo]; in the former the highest rate of degradation of racemic JH I11 was 0.5 pmol/CA/h [7].…”

Section: Jh Degradation By Cc-camentioning

confidence: 99%

“…This suggestion is supported by the observation that the addition of lipophorin (obtained through KBr gradient centrifugation) to incubations with CC-CA and (10R)-[3H]JH I11 significantly decreased rates of (10R)-[3H]JH I11 metabolism (unpublished data). With respect to the identity of the JH metabolites, the major product formed by CA of Munducu [7] and by CC-CA of Galleria [10,11] was JH acid (identified by TLC); in the latter species JH acid represented at least 95% of the degradation products.…”

Section: Jh Degradation By Cc-camentioning

confidence: 99%

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Degradation of juvenile hormone and methylation of juvenile hormone acid by corpora cardiaca–corpora allata of the cockroach, Nauphoeta cinerea: I. Biochemical aspects

Meyer

Lanzrein

1989

Arch Insect Biochem Physiol

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Corpora cardiaca‐corpora allata (CC‐CA) from vitellogenic females of Nauphoeta cinerea degraded, in vitro, racemic and (10R)‐juvenile hormone III (JH III) at a rate of 249 pmol/CC‐CA/h and 786 pmol/CC‐CA/h, respectively. The major metabolite formed was JH III acid, together with some highly polar products. CC‐CA homogenates degraded racemic JH III to a small extent, whereas (10R)‐JH III was degraded efficiently to JH III acid. No highly polar products were formed by CC‐CA homogenates. When CC‐CA were incubated with racemic JH III acid, some of this substance was degraded to highly polar products, and a minor part was methylated to JH III. CC degraded very little JH III acid and did not methylate it to JH III. CC‐CA homogenates methylated JH III acid very efficiently; we measured an apparent Kmax of 37.8 μM and a Vmax of 1,260 pmol/4 h/ CC‐CA equivalent. The addition of JH III acid to CC‐CA in vitro increased the rate of biosynthesis of JH III, as determined by measuring incorporation of methyl[14C]methionine into JH III. These data indicate that the metabolite JH III acid can enter the CA and be methylated to JH III.

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Section: Jh Degradation By Cc-camentioning

confidence: 99%

mentioning

confidence: 90%

Section: Jh Degradation By Cc-camentioning

confidence: 99%

Section: Jh Degradation By Cc-camentioning

confidence: 99%

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Degradation of juvenile hormone and methylation of juvenile hormone acid by corpora cardiaca–corpora allata of the cockroach, Nauphoeta cinerea: I. Biochemical aspects

Meyer

Lanzrein

1989

Arch Insect Biochem Physiol

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“…3 8.3 7.8 7.2 6.6 6.2 5.75.0 4.Wide range (pH 3-10) isoelectric focusing profiles of V4 hemolymph-]HE (H) and V6 CA-JHE ( 0 ) activities. Three primary peaks (pls 6.4,6.0-5.9, and 5.6) of hemolymph-JHE activity are apparent.…”

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Juvenile hormone esterase activity from Manduca sexta corpora allata in vitro

Sparks

Allen

Schneider

et al. 1989

Arch Insect Biochem Physiol

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Juvenile hormone esterase (JHE) activity released by the corpora allata (CA) into incubation media (CA-JHE) was titered daily during the course of the last (fifth [V]) larval stadium of Manduca sexta. This CA-JHE activity was relatively low during the early last stadium up to the time of commitment (V4), then rose rapidly to a peak on V6. Activity declined sharply almost to precommitment levels by V8, before rising to a second peak o n the first day of the pupal phase (PO). This pattern of activity i s distinct from that of hemolymph JHE activity, which peaks just prior to wandering o n V4 and again just prior to pupation (V9). Although the CA-JHE and hemolymph-JHE possess different temporal patterns of activity, isoelectric focusing, gel electrophoresis, and initial studies with selected inhibitors suggest that the enzymes responsible for the CA-JHE and hemolymph-JHE activities are similar, but not identical, in nature.Exposure of the V6 CA in vitro to JH II (0.1 pM) or fluoromevalonolactone (FMev; 0.1 mM) produced an approximate fivefold increase and 60% decrease in JH acid synthesis, respectively. Conversely, the same treatments resulted in an inhibition (JH II) and stimulation (FMev) of CA-JHE activity. These observations suggest that JH may be involved in the direct positive feedback regulation of postwandering larval CA and that the CA-JHE may also be integrally related to this positive feedback mechanism.Key words: endocrine feedback, hemolymph JH esterase, fluoromevalonolactone Acknowledgments: The authors thank Louise Studley for rearing the insects, Charles Allen and William Janzen for their help with the dissections, and Jay P. Woodring, Abner Hammond, Tad Hardy, and R. Mike Roe for their very useful discussions and manuscript comments.

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JH Secreting Activity of Corpora Allata from Fourth and Fifth Instar Larvae of Bombyx Mori

Nariki¹,

Kimura²,

Ohtaki³

1988

Invertebrate and Fish Tissue Culture

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Biosynthesis and degradation of juvenile hormone in corpora allata and imaginal wing discs of Galleria mellonella (L.)

Cited by 24 publications

References 30 publications

Degradation of juvenile hormone and methylation of juvenile hormone acid by corpora cardiaca–corpora allata of the cockroach, Nauphoeta cinerea: I. Biochemical aspects

Degradation of juvenile hormone and methylation of juvenile hormone acid by corpora cardiaca–corpora allata of the cockroach, Nauphoeta cinerea: I. Biochemical aspects

Juvenile hormone esterase activity from Manduca sexta corpora allata in vitro

JH Secreting Activity of Corpora Allata from Fourth and Fifth Instar Larvae of Bombyx Mori

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