Biosynthesis and characterization of zinc oxide nanoparticles using Nigella sativa against coccidiosis in commercial poultry

Lail, Najam-Ul; Sattar, Adeel; Omer, Muhammad Ovais; Hafeez, Mian Abdul; Khalid, Abdur Rauf; Mahmood, Sammina; Shabbir, Muhammad Abu Bakr; Ahmed, Waqas; Aleem, Muhammad Tahir; Alouffi, Abdulaziz; Almutairi, Mashal M.

doi:10.1038/s41598-023-33416-4

Cited by 10 publications

(3 citation statements)

References 81 publications

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“…Using zinc oxide nanoparticles (ZNPs), Lail et al [27] studied their anticoccidial properties in vivo. Upon characterization, ZNPs were revealed to be crystalline in nature, with a smooth and spherical surface as well as a diameter ranging between 10 and 15 nm.…”

Section: Discussionmentioning

confidence: 99%

Highlighting on The role of Zinc Oxide and Silver Nanoparticles as An Effective Coccidiostats in Broilers

Yousef,

Youssef,

Mohamed

et al. 2024

Egyptian Journal of Veterinary Sciences

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coccidiosis [3]. There are several species of Eimeria.spp. Each species has its own host and site, so any animal can host several of them [3]. Life cycles of Eimeria consist of two phases: sporogony, an exogenous phase, and schizogony, an endogenous phase. During the first phase of infection, gut cells are destroyed in large numbers by sporozoites, which are banana-shaped motile cells with complex structures that are capable of invading host cells [5].Sporozoites are the result of protoplasm R ECENT advances in nanomedicine have made it an attractive option for treating a range of diseases. Zinc oxide nanoparticles (ZnO NP) and silver nanoparticles (AgNP) were examined for their anticoccidial properties in vivo. This study aimed to highlight the effectiveness of zinc oxide and silver nanoparticles in preventing coccidiosis in broilers. In this study, Eimeria oocysts were synthesized, characterized, and analyzed histopathologically. TEM (transmission electron microscope) and SEM (scanning electron microscope) were used to characterize Zinc oxide nanoparticles enclosed in nanospheres of uniform spherical shape and size, while produced Ag nanoparticles were spherical. They showed high zeta potential, which indicates high colloidal stability. ZnO nanoparticles prepared by X-Ray diffraction analysis showed a high crystalline structure. Chemical homogeneity was evident from the XRD curves of AgNPs and ZnO NPs. The obtained size of Ag-NPs matched that of TEM, SEM and zeta potential values, demonstrating excellent stability in aqueous solutions. According to PCR tests, the prepared ZnO NPs and Ag-NPs have superior efficacy and potential compared to other anticoccidials. Zinc oxide nanoparticles showed potential anticoccidial efficacy compared to other groups. Results were confirmed by Histopathological examination.

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Section: Discussionmentioning

confidence: 99%

Highlighting on The role of Zinc Oxide and Silver Nanoparticles as An Effective Coccidiostats in Broilers

Yousef,

Youssef,

Mohamed

et al. 2024

Egyptian Journal of Veterinary Sciences

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“…CALPR has antimicrobial properties and can inhibit the growth and survival of pathogens, including coccidia ( 27 ). CALPR can also modulate the host inflammatory response by regulating the production of pro-inflammatory cytokines and chemokines, preventing excessive inflammation while promoting an effective defense against coccidia ( 28 , 29 ). In the current study, CALPR expression in the ileum, where E .…”

Section: Discussionmentioning

confidence: 99%

Assessing the effects of a mixed Eimeria spp. challenge on performance, intestinal integrity, and the gut microbiome of broiler chickens

Graham,

Petrone-Garcia,

Hernandez-Velasco

et al. 2023

Front. Vet. Sci.

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A mixed Eimeria spp. challenge model was designed to assess the effects of challenge on broiler chicken performance, intestinal integrity, and the gut microbiome for future use to evaluate alternative strategies for controlling coccidiosis in broiler chickens. The experimental design involved broiler chickens divided into two groups: a control group (uninfected) and a positive control group, infected with Eimeria acervulina (EA), Eimeria maxima (EM), and Eimeria tenella (ET). At day-of-hatch, 240 off-sex male broiler chicks were randomized and allocated to one of two treatment groups. The treatment groups included: (1) Non-challenged (NC, n = 5 replicate pens); and (2) challenged control (PC, n = 7 replicate pens) with 20 chickens/pen. Pen weights were recorded at d0, d16, d31, d42, and d52 to determine average body weight (BW) and (BWG). Feed intake was measured at d16, d31, d42, and d52 to calculate feed conversion ratio (FCR). Four diet phases included a starter d0–16, grower d16–31, finisher d31–42, and withdrawal d42–52 diet. At d18, chickens were orally challenged with 200 EA, 3,000 EM, and 500 ET sporulated oocysts/chicken. At d24 (6-day post-challenge) and d37 (19-day post-challenge), intestinal lesion scores were recorded. Additionally, at d24, FITC-d was used as a biomarker to evaluate intestinal permeability and ileal tissue sections were collected for histopathology and gene expression of tight junction proteins. Ileal and cecal contents were also collected to assess the impact of challenge on the microbiome. BWG and FCR from d16–31 was significantly (p < 0.05) reduced in PC compared to NC. At d24, intestinal lesion scores were markedly higher in the PC compared to the NC. Intestinal permeability was significantly increased in the PC group based on serum FITC-d levels. Cadherin 1 (CDH1), calprotectin (CALPR), and connexin 45 (Cx45) expression was also upregulated in the ileum of the PC group at d24 (6-day post-challenge) while villin 1 (VIL1) was downregulated in the ileum of the PC group. Additionally, Clostridium perfringens (ASV1) was enriched in the cecal content of the PC group. This model could be used to assess the effect of alternative coccidiosis control methods during the post-challenge with EA, EM, and ET.

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“…Ультрадисперсная форма имеет большую удельную поверхность, что обуславливает высокую каталитическую активность, биодоступность и соответственно усиленное взаимодействие с бактериальной мембраной [9]. Помимо этого, цинк обладает противогрибковой, противопаразитарной, антидиабетической, иммуномодулирующей активностью и используются в качестве стимулятора роста [10].…”

Section: введение/ Introductionunclassified

The effect of an enzyme additive in combination with zinc in an ultrafine form on the productive performance of broiler chickens

Nechitailo,

Sizova,

Ryazantseva

et al. 2023

jour

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Poultry farming is one of the most important and promising branches of animal husbandry. Given the increasing rate of industrial poultry farming, for the stable development of the industry and improving competitiveness in world markets, more and more attention is paid to the search for safe alternative agents to increase productivity. The study examined the strategy of feeding substances of different nature as feed additives, in particular, enzyme complexes separately (group I (0,05%; endo-1,4-beta-xylanase - 4000 units/g, alpha-amylase - 400 units/g and subtilisin - 8000 units/g)) and in combination with ultradispersed zinc (group II (3 mg/kg; 164±31,2 nm)). It was found that in group I the digestibility ratios of crude protein were higher by 6.03 % (P≤0.05), crude fiber by 3.68 % (P≤0.05), nitrogen-free extractive substances by 2.39 % (P≤0.05) compared to control. At the same time, in group II, changes were recorded in crude protein and crude fiber - an increase of 6.51% (P≤0.01) and 7.59% (P≤0.05), respectively. At 42 days of age, the live weight of broiler chickens I was 10.1% higher than control (P≤0.05), and in group II by 11.32% (P≤0.01). At the same time, the cost of feed per 1 kg of growth was minimal in group II, 16.79% lower than the control values. Thus, the maximum productive effect was observed with the introduction of a complex enzyme supplement in combination with zinc in ultradispersed form.

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Biosynthesis and characterization of zinc oxide nanoparticles using Nigella sativa against coccidiosis in commercial poultry

Cited by 10 publications

References 81 publications

Highlighting on The role of Zinc Oxide and Silver Nanoparticles as An Effective Coccidiostats in Broilers

Highlighting on The role of Zinc Oxide and Silver Nanoparticles as An Effective Coccidiostats in Broilers

Assessing the effects of a mixed Eimeria spp. challenge on performance, intestinal integrity, and the gut microbiome of broiler chickens

The effect of an enzyme additive in combination with zinc in an ultrafine form on the productive performance of broiler chickens

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