Biosynthesis and characterization of silver nanoparticles induced by fungal proteins and its application in different biological activities

Abstract

BackgroundThe present study aims to apply an efficient eco-friendly and inexpensive process for green synthesis of silver nanoparticles (AgNPs) through the mediation of fungal proteins from Aspergillus fumigatus DSM819, characterization, and its application as antimicrobial finishing agent in textile fabrics against some infectious microorganisms.ResultsOptimum conditions for AgNP biosynthesis could be achieved by means of using 60% (v/v) of cell-free filtrate (CFF) and 1.5 mM of AgNO3 at pH 10.0 after 90 min. The obtained AgNPs were of spherical shape with 90% of distribution below than 84.4 nm. The biosynthesized AgNPs exerted an antimicrobial activity against the studied pathogenic microorganisms (E. coli, B. mycoides, and C. albicans). In addition, IC50 values against in vitro tumor cell lines were found to be 31.1, 45.4, 40.9, and 33.5 μg/ml for HCT116, A549, MCF7, and PC3, respectively. Even with a very low concentration (0.25%), the treated PET/C fabrics by AgNPs exerted an antimicrobial activity against E. coli, B. mycoides, and C. albicans to give inhibition zone diameter of 15, 15, and 16 mm, respectively.ConclusionsThe green biosynthesis approach applied in this study is a non-toxic alternative to the traditional chemical and physical methods, and would be appropriate for biological large-scale production and prospective treatments.Graphical abstract

Endophytic Streptomyces laurentii Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties

“…Therefore, the treated fabric lost its activity through successive washing. Consistent with our results, Othman et al [ 59 ] revealed the application of fungal induced Ag-NPs for finishing durable antimicrobial fabrics and reported the activity of Ag-fabrics against Escherichia coli, Bacillus mycoides and Candida albicans . They added that the cloth retained the silver nanomaterials even after five repeated wash cycles.…”

“…These results proved the deposition of fine silver particles on the fabric surface ( Figure 7 ). Consistent with the current study, Othman et al [ 59 ] reported the successful use of biogenic colloidal silver as a nano-finishing agent for antimicrobial textiles. Their EDX analysis confirmed the presence of elemental silver with the strong peaks recorded at 3.0 keV.…”

Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics

“…Therefore, it is expected that the nano-finished fabrics will lose their antibacterial activity after repeated washing. In line with our results, Othman et al [ 81 ] used fungus-derived colloidal silver to functionalize fabrics and reported the activity of the processed fabric against Candida albicans, Bacillus mycoides, and Escherichia coli , as well as the durability of the nano-finished fabrics and their stability after five washing cycles. Moreover, our data were compatible with those of El-Naggar et al [ 52 ], who reported the efficacy of cotton fabrics loaded with Ag-NPs synthesized by Streptomyces sp.…”

“…Accordingly, the SEM/EDX results confirmed the existence of Ag-NPs attached to the textile surface. In line with the current study, Othman et al [ 81 ] reported the efficient application of bio-fabricated Ag-NPs for functionalizing nano-finished fabrics with antimicrobial properties; their EDX spectra showed a strong peak around 3.0 keV, thus demonstrating the deposition of nano-sized silver on the textile surface. Our results showed that the bio-silver nanoparticles effectively integrated into the surface of the treated fabrics and formed a reinforced surface layer; thus, they can be used as finishing materials for the multifunctional fabrics.…”

Correction to: Biosynthesis and characterization of silver nanoparticles induced by fungal proteins and its application in different biological activities

“…In the publication of this article [1], the title of Figure 6 was missing. The original article has been corrected.…”