Biosynthesis and characterization of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus

Doi, Yoshiharu; Segawa, Atsushi; Kunioka, Masao

doi:10.1016/0141-8130(90)90061-e

Cited by 206 publications

(100 citation statements)

References 28 publications

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“…In these anaerobic bacteria 4-hydroxybutyrate is converted to 3-hydroxybutyryl-CoA via vinylacetyl-CoA and crotonyl-CoA by the enzymes 4-hydroxybutyrate CoA : CoA transferase, 4-hydroxybutyryl-CoA dehydratase and crotonylCoA hydratase (Kenealy and Waselewsky, 1985;Sohling and Gottschalk, 1993a). On this background a similar pathway was predicted for the degradation of 4-hydroxybutyrate to occur in A. eutrophus HI6 (Doi et al, 1990b). Alternatively, under aerobic conditions, 4-hydroxybutyrate may be degraded to succinate via succinate semialdehyde such as in Pseudomonas sp.…”

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confidence: 98%

Metabolic Pathway for Biosynthesis of Poly (3‐Hydroxybutyrate‐co‐4‐Hydroxybutyrate) from 4‐Hydroxybutyrate by Alcaligenes eutrophus

Valentin

Zwingmann

Schönebaum

et al. 1995

European Journal of Biochemistry

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Various aerobic Gram-negative bacteria have been examined for their ability to use 4-hydroxybutyrate and 1,Cbutanediol as carbon source for growth. Alcaligenes eutrophus strains H16, HF39, PHB-4 and Pseudomonas denitrificans 'Morris' were not able to grow with 1,4-butanediol or 4-hydroxybutyrate. From A. eutrophus HF39 spontaneous primary mutants (e. g. SK4040) were isolated which grew on 4-hydroxybutyrate with doubling times of approximately 3 h. Tn5: :mob mutagenesis of mutant SK4040 led to the isolation of two phenotypically different classes of secondary mutants which were affected in the utilization of 4-hydroxybutyrate. Mutants exhibiting the phenotype 4-hydroxybutyrate-negative did not grow with 4-hydroxybutyrate, and mutants exhibiting the phenotype 4-hydroxybutyrate-leaky grew at a significantly lower rate with 4-hydroxybutyrate. Hybridization experiments led to the identification of a 10-kbp genomic EcoRI fragment of A. eutrophus SK4040, which was altered in mutants with the phenotype 4-hydroxybutyrate-negative, and of two 1-kbp and 4.5-kbp genomic EcoRI fragments, which were altered in mutants with the phenotype 4-hydroxybutyrate-leaky. This 1 0-kbp EcoRI fragment was cloned from A. eutrophus SK4040, and conjugative transfer of a pVDZ'2 hybrid plasmid to A. eutrophus H16 conferred the ability to grow with 4-hydroxybutyrate to the wild type. DNA-sequence analysis of this fragment, enzymic analysis of the wild type and of mutants of A. eutrophus as well as of recombinant strains of Escherichia coli led to the identification of a structural gene encoding for a 4-hydroxybutyrate dehydrogenase which was affected by transposon mutagenesis in five of six available 4-hydroxybutyratenegative mutants. Enzymic studies also provided evidence for the presence of an active succinate-semialdehyde dehydrogenase in 4-hydroxybutyrate-grown cells. This indicated that degradation of 4-hydroxybutyrate occurs via succinate semialdehyde and succinate and that the latter is degraded by the citric acid cycle. NMR studies of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) accumulated from 4-hydroxy [I-"C]butyrate or 4-hydro~y[2-'~C]butyrate as substrate gave no evidence for a direct conversion of 4-hydroxybutyrate into 3-hydroxybutyrate and therefore supported the results of enzymic analysis.

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confidence: 98%

Metabolic Pathway for Biosynthesis of Poly (3‐Hydroxybutyrate‐co‐4‐Hydroxybutyrate) from 4‐Hydroxybutyrate by Alcaligenes eutrophus

Valentin

Zwingmann

Schönebaum

et al. 1995

European Journal of Biochemistry

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“…Embora não tenha havido diferença no grau de cristalinidade dos copolímeros, a taxa de degradação do P(3HB-co-9 % 4HB) foi maior do que a do P(3HB) e do P(3HB-co-50 % 3HV), ou seja, o fator determinante para biodegradação foi a composição do polímero. Doi et al (1990) relataram resultados semelhantes para copolímeros contendo unidades monoméricas de 4HB. Os autores observaram que em lodo aerado ativado, a 30 °C, os filmes de 3HB-co-9 % 4HB foram completamente decompostos em 14 dias, enquanto o P(3HB) levou mais de 10 semanas para se decompor.…”

Section: Biodegradação De Polímerosunclassified

“…Essa conclusão foi ratificada pelos experimentos de Doi et al (1990), que mostraram que a perda de massa foi inversamente proporcional ao grau de cristalinidade. Por outro lado, Doi et al (1990) demonstraram que a taxa de degradação hidrolítica, diferentemente da degradação enzimática, não é dependente da cristalinidade do polímero e sim da sua composição química. Conclui-se, portanto, que a taxa de degradação dos polímeros varia dependendo do ambiente em que se encontra a amostra (umidade, disponibilidade de nutrientes, pH), aumentando em função da temperatura (Mergaert et al, 1993), do grau de cristalinidade, da massa molar do polímero e da incorporação de unidades monoméricas na cadeia polimérica (SUDESH et al, 2000).…”

Section: Biodegradação De Polímerosunclassified

SÍNTESE, CARACTERIZAÇÃO E DEGRADAÇÃO DE P(3HB) POR Cupriavidus necator UTILIZANDO GLICEROL COMO SUBSTRATO

Apati¹,

Schneider²,

Sombrio³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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“…Several inherent deficiencies of PHB have limited its medical applications, including the brittleness due to the high crystallinity and the narrow thermal processing window because of the thermal instability. PHA copolyesters containing, besides 3-hydroxybutyrate (3HB, Figure 1a), 4-hydroxybutyrate (4HB, Figure 1b) [9][10][11] Figure 1d) [17][18], hydroxypropionate (HP, Figure 1e) [19][20], hydroxyhexanoate (HHx, Figure 1f) [21][22][23], and hydroxyoctanoate (HO, Figure 1g) [24] units can be produced by different microorganisms. Varying the copolymer composition affords obtaining copolyesters with adjustable mechanical and processing properties exceeding those of the P3HB homopolymer.…”

Section: Introductionmentioning

confidence: 99%

Synthetic routes to degradable copolymers deriving from the biosynthesized polyhydroxyalkanoates: A mini review

Ke¹,

Zhang²,

Ramakrishna³

et al. 2016

Express Polym. Lett.

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Abstract.Polyhydroxyalkanoates are a family of natural polyesters being produced as intracellular carbon and energy reserves by a wide variety of microorganisms. They have developed rapidly in both research and development efforts globally in the last 15 years. Till now, over 100 different types of PHAs have been successfully biosynthesized using both genetic engineering and fermentation techniques. Their unique biodegradable, biocompatible and thermoplastic characteristics make PHAs promising candidates for the commodity and biomedical applications. This review focused on the chemical synthesis of the derivatives of the biosynthesized PHAs.

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Biosynthesis and characterization of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus

Cited by 206 publications

References 28 publications

Metabolic Pathway for Biosynthesis of Poly (3‐Hydroxybutyrate‐co‐4‐Hydroxybutyrate) from 4‐Hydroxybutyrate by Alcaligenes eutrophus

Metabolic Pathway for Biosynthesis of Poly (3‐Hydroxybutyrate‐co‐4‐Hydroxybutyrate) from 4‐Hydroxybutyrate by Alcaligenes eutrophus

SÍNTESE, CARACTERIZAÇÃO E DEGRADAÇÃO DE P(3HB) POR Cupriavidus necator UTILIZANDO GLICEROL COMO SUBSTRATO

Synthetic routes to degradable copolymers deriving from the biosynthesized polyhydroxyalkanoates: A mini review

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