Biosurfactants production by yeasts using soybean oil and glycerol as low cost substrate

Abstract

Biosurfactants are bioactive agents that can be produced by many different microorganisms. Among those, special attention is given to yeasts, since they can produce many types of biosurfactants in large scale, using several kinds of substrates, justifying its use for industrial production of those products. For this production to be economically viable, the use of residual carbon sources is recommended. The present study isolated yeasts from soil contaminated with petroleum oil hydrocarbons and assessed their capacity for producing biosurfactants in low cost substrates. From a microbial consortium enriched, seven yeasts were isolated, all showing potential for producing biosurfactants in soybean oil. The isolate LBPF 3, characterized as Candida antarctica, obtained the highest levels of production - with a final production of 13.86 g/L. The isolate LBPF 9, using glycerol carbon source, obtained the highest reduction in surface tension in the growth medium: approximately 43% of reduction after 24 hours of incubation. The products obtained by the isolates presented surfactant activity, which reduced water surface tension to values that varied from 34 mN/m, obtained from the product of isolates LBPF 3 and 16 LBPF 7 (respectively characterized as Candida antarctica and Candida albicans) to 43 mN/m from the isolate LPPF 9, using glycerol as substrate. The assessed isolates all showed potential for the production of biosurfactants in conventional sources of carbon as well as in agroindustrial residue, especially in glycerol

Effects of Glucose and Ferrous Supplements and Culture Conditions on Lipopeptide Biosurfactant from Pseudomonas spp.

“…After 24 h culture time, the surface tension was mostly constant at 30 mN/m. The result was agreed with P. aeruginosa MA01 [17] and Candida albicans [18]. Also, Rufino et al [19] reported that C. lipolytica UCP0988 produced rufisan biosurfactant during exponential growth phase.…”

Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates

“…New prospects for industrial production are focused on biotechnological processes that use agro-industrial wastes as substrates in formulating alternative medium in fermentation processes for producing secondary metabolites with a view to decreasing the costs of production (Accorsini et al, 2012;Antunes et al, 2013;Yasmin et al, 2016). In addition, this market shows promise to attract interest in the studies of biosurfactants that can be used as raw material in applications in the food-, agrochemical, cosmetics and pharmaceuticalindustries (Morikawa et al, 2000;Luna et al, 2009;Santos et al, 2016).…”

IDENTIFICATION OF <i>PSEUDOZYMA HUBEIENSIS</i> Y10BS025 AS A POTENT PRODUCER OF GLYCOLIPID BIOSURFACTANT <i>MANNOSYLERYTHRITOL LIPIDS</i>

“…To overcome the expensive cost constraints associated with MEL's production, the use of inexpensive and waste substrates for the formulation of fermentation media has been suggested (Saharan et al, 2011). Accorsini et al (2012) used soybean oil and glycerol as low cost substrates for biosurfactant production by yeast. The use of olive oil for MEL's production has also been reported (Morita et al, 2009c).…”