Biosurfactants production by yeasts using soybean oil and glycerol as low cost substrate

Accorsini, Fábio Raphael; Mutton, Márcia Justino Rossini; Lemos, Eliana Gertrudes Macedo; Benincasa, Maria

doi:10.1590/s1517-83822012000100013

Cited by 60 publications

(27 citation statements)

References 34 publications

(11 reference statements)

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“…After 24 h culture time, the surface tension was mostly constant at 30 mN/m. The result was agreed with P. aeruginosa MA01 [17] and Candida albicans [18]. Also, Rufino et al [19] reported that C. lipolytica UCP0988 produced rufisan biosurfactant during exponential growth phase.…”

Section: E Pseudomonas Spp Growth Kineticssupporting

confidence: 71%

Effects of Glucose and Ferrous Supplements and Culture Conditions on Lipopeptide Biosurfactant from Pseudomonas spp.

Choopraserdchok¹,

Athinuwat²,

Cheunchomrat³

2018

IJESD

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Abstract-To enhance lipopetide biosurfactant production by Pseudomonas spp., the effects of medium and culture conditions were investigated. Nutrient broth (NB) supplemented with glucose and molasses as carbon sources were used to increase biosurfactant yield. Cultivation temperatures and initial pH were also studied. In this experiment, NB was used as a control to produce biosurfactant with the yield of 0.58 g/L. The lipopeptide biosurfactant yield was increased to 2.76 g/L when Pseudomonas spp. was cultured in NB supplemented with 1% w/v glucose, 500 µM FeSO4, pH 9.0 and cultivation of 20°C for 72 h. Its specific growth rate was 0.25 h-1.Index Terms-Pseudomonas spp., lipopeptide biosurfactant, glucose, ferrous.

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Section: E Pseudomonas Spp Growth Kineticssupporting

confidence: 71%

Effects of Glucose and Ferrous Supplements and Culture Conditions on Lipopeptide Biosurfactant from Pseudomonas spp.

Choopraserdchok¹,

Athinuwat²,

Cheunchomrat³

2018

IJESD

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“…New prospects for industrial production are focused on biotechnological processes that use agro-industrial wastes as substrates in formulating alternative medium in fermentation processes for producing secondary metabolites with a view to decreasing the costs of production (Accorsini et al, 2012;Antunes et al, 2013;Yasmin et al, 2016). In addition, this market shows promise to attract interest in the studies of biosurfactants that can be used as raw material in applications in the food-, agrochemical, cosmetics and pharmaceuticalindustries (Morikawa et al, 2000;Luna et al, 2009;Santos et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates

Lima¹,

Andrade²,

RodrÃguez³

et al. 2017

Afr. J. Microbiol. Res.

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The world market for biosurfactants has grown gradually. However, the lack of competitiveness with chemical surfactants due to high cost of production remains a concern. Considering the need to reduce the costs of production, the aim of this work was to study the production and structural characterization of a biosurfactant produced by a strain of yeast Candida glabrata UCP 1556. The lowcost medium containing agro-industrial wastes whey 40% (v/v) and 20% (v/v) corn steep liquor were used as substrates in submerged fermentation. Biosurfactant production was detected by surface tension, oil displacement test and Critical Micelle Concentration (CMC). The structural characterization was performed by Fourier transform infrared (FT-IR), gas chromatography-mass spectrometry (GC-MS) and ionic profile. The stability of emulsions and potential in reducing the viscosity also were investigated. The results showed that the biosurfactant reduced the surface tension to 28.8 mN/m with CMC of 2% and showed anionic profile. Additionally, the biosurfactant formed stable emulsions at temperature (0 to 120°C), pH (2 to 12) and NaCl (2 to 12%), reduced the viscosity of soybean oil (

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“…To overcome the expensive cost constraints associated with MEL's production, the use of inexpensive and waste substrates for the formulation of fermentation media has been suggested (Saharan et al, 2011). Accorsini et al (2012) used soybean oil and glycerol as low cost substrates for biosurfactant production by yeast. The use of olive oil for MEL's production has also been reported (Morita et al, 2009c).…”

Section: Discussionmentioning

confidence: 99%

IDENTIFICATION OF <i>PSEUDOZYMA HUBEIENSIS</i> Y10BS025 AS A POTENT PRODUCER OF GLYCOLIPID BIOSURFACTANT <i>MANNOSYLERYTHRITOL LIPIDS</i>

Sari¹,

Kanti²,

Artika

et al. 2013

American Journal of Biochemistry and Biotechnology

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Mannosylerythritol Lipids (MEL's) are glycolipid biosurfactants that contain 4-O-β-D-mannopyranosylmeso-erythritol as a hydrophilic moiety and fatty acids as a hydrophobic moiety. MEL's are abundantly produced by several kinds of microorganism and are one of the most promising biosurfactants currently known. The search for a novel endogenous producer of MEL's was undertaken based on the available collection of the yeast strains from the genus Pseudozyma. Using thin layer chromatography and based on morphological and molecular taxonomic analysis using the D1/D2 domains of the large subunit 26S rRNA gene, Pseudozyma hubeiensis Y10BS025 was found to be a potential producer of MEL's from soybean oil. The structure of the major glycolipid produced by the strain was analyzed by 1 H and 13 C nuclear magnetic resonance and was found to be similar to those of well known MEL-A, -B and -C respectively. Under improved shaking culture conditions, using yeast extract as nitrogen source and soybean oil as substrate, a maximum yield of 115±3.2 g.L −1 of MEL's for 8 days of fermentation was achieved. The major fatty acids of MEL's produced by P. hubeiensis Y10BS025 were C-18 acids, considerably different from those of MEL-C produced by other Pseudozyma strains such as P. antarctica and P. shanxiensis. The main product, MEL-C produced by P. hubeiensis Y10BS025 exhibited surface-tension-lowering activity. The results demonstrated that the newly isolated P. hubeiensis Y10BS025 provided high efficiency in MEL's production and would thus be highly advantageous in commercial production of promising biosurfactants.

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Biosurfactants production by yeasts using soybean oil and glycerol as low cost substrate

Cited by 60 publications

References 34 publications

Effects of Glucose and Ferrous Supplements and Culture Conditions on Lipopeptide Biosurfactant from Pseudomonas spp.

Effects of Glucose and Ferrous Supplements and Culture Conditions on Lipopeptide Biosurfactant from Pseudomonas spp.

Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates

IDENTIFICATION OF <i>PSEUDOZYMA HUBEIENSIS</i> Y10BS025 AS A POTENT PRODUCER OF GLYCOLIPID BIOSURFACTANT <i>MANNOSYLERYTHRITOL LIPIDS</i>

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