Biosurfactant, solvents and polymer production by Bacillus subtilis RI4914 and their application for enhanced oil recovery

One Biosurfactant-Producing Bacteria Achromobacter sp. A-8 and Its Potential Use in Microbial Enhanced Oil Recovery and Bioremediation

“…Strain A-8 formed larger oil displacement diameters when (NH 4 ) 2 SO 4 was used as the nitrogen source compared with the other three nitrogen sources (Figure 4B). A similar phenomenon was observed in the strain B. subtilis RI4914, for which NH 4 NO 3 was the best nitrogen source for biosurfactant production (Fernandes et al, 2016). The largest oil displacement diameter in the temperature tests occurred at 40-45 • C, while the best pH for biosurfactant production was about 7 (Figures 4C,D).…”

“…Microbial enhanced oil recovery was first proposed by Beckman (1926), but this technology was slowly developed during a long period of time due to the limits of science and technology (Brown, 2010). As a key point of MEOR, the finding of proper microorganisms had attracted a lot of concentrations and many bacteria strains were isolated and characterized in laboratory (Al-Wahaibi et al, 2014;Fernandes et al, 2016;Zhang et al, 2016;Ke et al, 2019). The field tests were important for MEOR from laboratory experiments to industry application.…”

“…To date, numerous bacterial strains that can degrade petroleum or produce biosurfactant have been isolated from different oil-contaminated environments worldwide. Such strains include Chelatococcus daeguensis HB-4 from Baolige Oilfield in China (Ke et al, 2019), Rhodococcus erythropolis OSDS1 isolated from a solid waste management unit (SWMU) contaminated with petroleum hydrocarbons and heavy metals in the United States (Xia et al, 2019), Bacillus subtilis RI4914 from an oil field in Brazil (Fernandes et al, 2016), Bacillus licheniformis from oil-contaminated water samples in Egypt (El-Sheshtawy et al, 2015), Pseudomonas putida DB1 and Bacillus cereus DB2 isolated from petroleumcontaminated soil in India (Vinothini et al, 2015), and B. subtilis B30 from oil-contaminated soils in Oman (Al-Wahaibi et al, 2014). As an important part of MEOR and bioremediation, biosurfactant-producing strains have been studied under different conditions (e.g., pH, temperature, salinity, and nutrition) to find the optimal conditions for biosurfactant production.…”

Selection of the main micronutrients for the production of biosurfactant by an isolated Bacillus subtilis strain

“…Recent studies in the literature report that values of surface tension in the range obtained in this work indicate that the bioproduct may have efficient surfactant activity, even in contact with a complex mixture of hydrocarbons, such as crude oil. Fernandes et al [16] presented that the injection of 600mg/L of biosurfactant resulted in approximately 69% recovery of residual oil in a glass column (Kontes Flex Column, Fisher Scientific K420401-2510) of 2.5x10 cm that remained after injection with water.…”

Biosurfactants as Promising Multifunctional Agent: A Mini Review

“…Currently, biosurfactants have gained much attention due to their advantages over the chemical surfactants in terms of their low toxicity, environmental capability, biodegradability, renewability, availability of natural resources, desirable functionality, and better availability for a wide range of pH values and temperatures [2,3]. Hence, these properties enabled them to be a promising component with a wide range of application in many industries, especially for heavy metal removal in contaminated soil [4][5][6], microbial enhanced oil recovery [7][8][9][10], therapeutic agent in cosmetic and pharmaceutical products [1,11,12].…”