Biosurfactant Production by Strains of Azospirillum Isolated from Petroleum-Contaminated Sites

Ojeda‐Morales, Marcia E.; Domínguez-Domínguez, Marivel; Hernández-Rivera, Miguel A.; Zavala-Cruz, Joel

doi:10.1007/s11270-015-2659-0

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Production of the Surfactant. Once the bioreactors were prepared, the A. lipoferum strain (Ojeda-Morales et al 2015) was inoculated for the production of the biomass and bacterial surfactant. The bacterial strain was registered in the Colegio de Postgraduados Campus Tabasco.…”

Section: Preparation Of Emulsified Oil Dosesmentioning

confidence: 99%

Do surfactants influence the growth of Rhizophora mangle during restoration of contaminated soil with emulsified oil?

Ojeda‐Morales

Domínguez-Domínguez

Herrera-Haro

et al. 2022

Restoration Ecology

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Mangrove forests are ecosystems subject to contamination by oil spills. The objective of this study was to evaluate the development of Rhizophora mangle plants in soil contaminated with oil emulsified with surfactants. These ecosystems have diverse and economically valuable ecological functions. Mangrove soil and propagules were collected in southeastern Mexico. The propagules were sown under greenhouse conditions and the plants were grown for 3 months. Two bioassays were applied: (1) the soil was contaminated by oil emulsified with a biosurfactant synthesized by Azospirillum lipoferum, and (2) the soil was contaminated with oil emulsified with a surfactant based on pine essential oil. Emulsified oil was applied to the substrate in containers with 3-month-old plants until several concentrations were reached (0, 30,000, 40,000, 50,000, 60,000, and 70,000 ppm). Subsequently, 5 plants for each treatment were randomly extracted every 30 days for 12 months, and the stem length and diameter, root length, leaf area, and fresh and dry weights of the plants were evaluated. Plant development was evaluated through an analysis of variance and a test of means. At the end of the bioassay, the treatments with oil emulsified with pine oil yielded no surviving plants, whereas the treatments with oil emulsified with the biosurfactant yielded a 100% survival rate. Treatment with 30,000 ppm of oil emulsified with the biosurfactant yielded the greatest increases in the leaf area and total dry biomass. The heavy oil fraction concentration decreased by 93.9% with the 30,000 ppm treatment and by 82.64% with the 70,000 ppm treatment.

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Section: Preparation Of Emulsified Oil Dosesmentioning

confidence: 99%

Do surfactants influence the growth of Rhizophora mangle during restoration of contaminated soil with emulsified oil?

Ojeda‐Morales

Domínguez-Domínguez

Herrera-Haro

et al. 2022

Restoration Ecology

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“…Estos microorganismos además enriquecen el suelo con nutrientes, restaurando su fertilidad cuando ha sido impactado por derrames de hidrocarburos. Algunos géneros han sido reportados en la literatura: Clostridium, Rhizobium, Azotobacter, Azospirillum, Beijerinkia (fijadoras de nitrógeno) y Pseudomonas, Bacillus, Flavobacteria, Aspergillus, Agrobacterium, Micrococcus, Achromobacter (solubilizadoras de fósforo) tanto en la degradación de hidrocarburos como productoras de biosurfactantes (Ojeda-Morales et al, 2015;Girigiri et al, 2020;Bhosale & Khode, 2021;Kumar et al, 2021). Dada la importancia de estos microorganismos, en esta investigación se aisló y caracterizó un biosurfactante de cepas bacterianas nativas de la rizósfera de plantas de cacao, que son fijadoras de nitrógeno y que además tienen potencial para su aplicación en la restauración de suelos impactados con hidrocarburos del petróleo.…”

Section: Introductionunclassified

Extraction and characterization of a biosurfactant synthesized by Acinotebacter baumannii

Torres-Custodio¹,

Hernández-Rivera²,

López-Lázaro³

et al. 2023

Mexjbiotechnol

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Biosurfactants are metabolites produced by microorganisms during their growth and reproduction. They are amphiphilic molecules capable of modifying surface and interfacial tension. A petrophilic, nitrogen-fixing, phosphorus-solubilizing, and biosurfactant-producing bacterial strain was isolated and characterized. The isolated strain was cultivated in Kim medium with a C:N ratio of 2:1, from which its growth kinetics was determined. Samples of the culture broth were taken at different times and the collapsed drop, oil dispersion and emulsification index tests were performed. Maximum culture growth occurred at 72 h. The biosurfactant was extracted from the crude cell-free broth using a mixture of solvents and a yield of 2.21 g/L was obtained. Thin-layer chromatography and FT-IR analyzes revealed the presence of a lipopeptide biosurfactant. Tensiometry tests showed that the biosurfactant was able to lower the surface tension of water from 72 mN/m to 40 mN/m and the critical micelle concentration was 0.058 (w/w). Probit analysis for toxicity determination showed that there was no weight loss in Eisenia foetida specimens in a range between 10000 and 50000 ppm.

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Factors Affecting Microbial Enhanced Oil Recovery (MEOR)

Rivera

Vasconcellos

Morales

2018

Proceedings of the 25th Pan-American Conference of Naval Engineering—COPINAVAL

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Biosurfactant Production by Strains of Azospirillum Isolated from Petroleum-Contaminated Sites

Cited by 5 publications

References 16 publications

Do surfactants influence the growth of Rhizophora mangle during restoration of contaminated soil with emulsified oil?

Do surfactants influence the growth of Rhizophora mangle during restoration of contaminated soil with emulsified oil?

Extraction and characterization of a biosurfactant synthesized by Acinotebacter baumannii

Factors Affecting Microbial Enhanced Oil Recovery (MEOR)

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