Biosurfactant production by Pseudomonas fluorescens 378: growth and product characteristics

Persson, Anders; Österberg, E.; Dostálek, Milan

doi:10.1007/bf00258342

Cited by 71 publications

(36 citation statements)

References 16 publications

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“…Only the two P. aeruginosa isolates were positive for the rhlB gene, and rhamnolipid production was confirmed by thin-layer chromatography analysis. Other Pseudomonas species have been reported to produce a variety of lipoproteins including viscosin, tensin, syringomycin, and syringopeptin, as well as polymeric biosurfactants such as Biosur-Pm and PM factor (10,21,23,32,39,41,43). The remaining 11 isolates obtained in this study were Bacillus spp.…”

Section: Discussionmentioning

confidence: 81%

Distribution of Biosurfactant-Producing Bacteria in Undisturbed and Contaminated Arid Southwestern Soils

Bodour

Drees

Maier

2003

Appl Environ Microbiol

314

173

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Biosurfactants are a unique class of compounds that have been shown to have a variety of potential applications in the remediation of organic-and metal-contaminated sites, in the enhanced transport of bacteria, in enhanced oil recovery, as cosmetic additives, and in biological control. However, little is known about the distribution of biosurfactant-producing bacteria in the environment. The goal of this study was to determine how common culturable surfactant-producing bacteria are in undisturbed and contaminated sites. A series of 20 contaminated (i.e., with metals and/or hydrocarbons) and undisturbed soils were collected and plated on R 2 A agar. The 1,305 colonies obtained were screened for biosurfactant production in mineral salts medium containing 2% glucose. Forty-five of the isolates were positive for biosurfactant production, representing most of the soils tested. The 45 isolates were grouped by using repetitive extragenic palindromic (REP)-PCR analysis, which yielded 16 unique isolates. Phylogenetic relationships were determined by comparing the 16S rRNA gene sequence of each unique isolate with known sequences, revealing one new biosurfactant-producing microbe, a Flavobacterium sp. Sequencing results indicated only 10 unique isolates (in comparison to the REP analysis, which indicated 16 unique isolates). Surface tension results demonstrated that isolates that were similar according to sequence analysis but unique according to REP analysis in fact produced different surfactant mixtures under identical growth conditions. These results suggest that the 16S rRNA gene database commonly used for determining phylogenetic relationships may miss diversity in microbial products (e.g., biosurfactants and antibiotics) that are made by closely related isolates. In summary, biosurfactant-producing microorganisms were found in most soils even by using a relatively limited screening assay. Distribution was dependent on soil conditions, with gram-positive biosurfactant-producing isolates tending to be from heavy metal-contaminated or uncontaminated soils and gram-negative isolates tending to be from hydrocarbon-contaminated or cocontaminated soils.

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Section: Discussionmentioning

confidence: 81%

Distribution of Biosurfactant-Producing Bacteria in Undisturbed and Contaminated Arid Southwestern Soils

Bodour

Drees

Maier

2003

Appl Environ Microbiol

314

173

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“…The decrease in E 24 % after 48 h of incubation shows that biosurfactant biosynthesis stopped and is probably due to the production of secondary metabolites which could impede with emulsion formation and the adsorption of surfactant molecules at the oil-water interface (Bonilla et al, 2005). These results indicate that the biosurfactant biosynthesis using molasses occurred predominantly during the exponential growth phase, suggesting that the biosurfactant is produced as primary metabolite accompanying dry cell weight as evidenced in the growth-associated kinetics (Persson et al, 1988). Previous studies reported that rhamnolipid biosurfactant was produced during the logarithmic and stationary phases of bacterial growth and amount of production increased after then (Zhang and Miller, 1995).…”

Section: Biosurfactant Production Kineticsmentioning

confidence: 91%

Rhamnolipid biosurfactant production by Pseudomonas aeruginosa strain KVD-HR42 isolated from oil contaminated mangrove sediments

Deepika¹,

Raghuram²,

Bramhachari³

2017

Afr. J. Microbiol. Res.

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Pseudomonas aeruginosa strain KVD-HR42 exhibiting growth and biosurfactant production with 1% molasses as the sole carbon source was isolated from oil contaminated mangrove sediments. Optimization of media conditions involving variations in carbon, nitrogen sources, amino acids, pH, temperature, and NaCl% were evaluated with the aim of increasing biosurfactant productivity and surface tension reduction (STR). The highest biosurfactant production of 4.83 g/L was obtained when cells were grown in mineral salts media (MSM) supplemented with 1% (w/v) molasses, NaNO 3 , and leucine 0.1% (w/v) at 35±2°C at 150 rpm after 48 h. The results obtained from kinetics study indicated that biosurfactant production, E 24 %, and rhamnose concentration were growth associated. However, maximum biosurfactant production occurred in the exponential growth phase and detected an increase in E 24 % and rhamnose concentration. The Fourier transform infrared (FTIR) spectra confirmed the rhamnolipid nature of the biosurfactant. Stability studies revealed the thermostable activity of biosurfactant (110°C for 15 min) and could also withstand wide pH and NaCl ranges. Maximum oil biodegradation of 68% was achieved with 1% waste lubricant oil (WLO). The biosurfactant emulsified various hydrocarbons with varied efficiencies. However maximum E 24 % and E 48 % activity was exhibited with n-hexadecane (69.5 and 40%). The results reveal the potential of strain KVD-HR42 biosurfactant for the bioremediation of petroleum hydrocarbons in mangrove sediments.

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“…Other pseudomonads such as Pseudomonas fluorescens have also been reported to produce rhamnolipids, particularly when glycerol is used as a carbon source (39), and Pseudomonas strains with the capacity to produce biosurfactants other than rhamnolipids when growing on sucrose were isolated from the environment by a simple screening procedure (31). One of these isolates, P. fluorescens 378, was shown to produce a novel surface-active compound of high molecular weight consisting mainly of carbohydrates and a protein with a molecular weight of 106 and an isoelectric point of 9.1 (32).…”

mentioning

confidence: 99%

Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants

Koch¹,

Käppeli²,

Fiechter³

et al. 1991

J Bacteriol

289

178

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We isolated transposon TnS-GM-induced mutants of Pseudomonas aeruginosa PG201 that were unable to grow in minimal media containing hexadecane as a carbon source. Some of these mutants lacked extracellular rhamnolipids, as shown by measuring the surface and interfacial tensions of the cell culture supernatants. Furthermore, the concentrated culture media of the mutant strains were tested for the presence of rhamnolipids by thin-layer chromatography and for rhamnolipid activities, including hemolysis and growth inhibition ofBacilus subtilis. Many procaryotic and eucaryotic microorganisms satisfy their carbon and energy requirements by using compounds, such as hydrocarbons, that are poorly soluble in aqueous media. The growth on hydrocarbons is often associated with the production of surface-active compounds. Surface-active molecules contain hydrophilic and hydrophobic components, a property that enables such molecules to concentrate at interfaces and to reduce the surface tensions of aqueous media. Several different microbial products that exhibit surface-active properties have been identified in the past. These so-called biosurfactants are produced by certain bacteria and by a number of yeasts and filamentous fungi. They include low-molecular-weight glycolipids, lipopeptides, and high-molecular-weight lipid-containing polymers such as lipoproteins, lipopolysaccharide-protein complexes, and polysaccharide-protein-fatty acid complexes. The common hydrophobic (lipophilic) moiety in biosurfactants is the hydrocarbon chain of a fatty acid, whereas the polar or hydrophilic group is derived from the ester or alcohol functional groups of neutral lipids, from the carboxylate group of fatty acids or amino acids, or, in the case of glycolipids, from carbohydrates. When the surfactants are extracellular, they cause the emulsification of the hydrocarbon. When they are cell wall associated, they facilitate the penetration of hydrocarbons to the periplasmic space. Many of the biosurfactants known today have been investigated with a view toward possible technical applications. Because biosurfactants are readily biodegradable and can be produced in large amounts by microorganisms and thus are not dependent on petroleum-derived products, they might well be able to replace, in some instances, the traditional synthetic surfactants. The structures, properties, and production of biosurfactants have been reviewed extensively; the overview of Reiser et al. (34) is the most recent. * Corresponding author.The rhamnose-containing glycolipids produced by Pseudomonas spp. (20,22,38) are among the biosurfactants to be studied most intensively. The rhamnolipids from Pseudomonas aeruginosa were first described in 1949 (24), and studies on the biosynthesis of these compounds were carried out in vivo by , who showed that these glycolipids were secreted into the medium during the stationary phase of growth. They also defined the optimal conditions for rhamnolipid production by this organism from various radioactive precursors, such as acetate, gly...

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Biosurfactant production by Pseudomonas fluorescens 378: growth and product characteristics

Cited by 71 publications

References 16 publications

Distribution of Biosurfactant-Producing Bacteria in Undisturbed and Contaminated Arid Southwestern Soils

Distribution of Biosurfactant-Producing Bacteria in Undisturbed and Contaminated Arid Southwestern Soils

Rhamnolipid biosurfactant production by Pseudomonas aeruginosa strain KVD-HR42 isolated from oil contaminated mangrove sediments

Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants

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