Biosurfactant Production By A New Pseudomonas Putida Strain

Tuleva, Borjana; Ivanov, G.; Christova, Nelly

doi:10.1515/znc-2002-3-426

Cited by 138 publications

(79 citation statements)

References 2 publications

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“…Pseudomonas aeruginosa has been widely studied and is known to produce a glycolipid type of biosurfactant [34,35]. Rhamnolipids are amphiphilic surface-active glycolipids that are usually secreted in the growth medium [36,37], but can potentially be used to combat marine oil pollution [8]. Figure 5 shows that the value of MATH increased from 0.71 to 0.91 when B1 was cultured with hexadecane.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of n-hexadecane by bacterial strains B1 and B2 isolated from petroleum-contaminated soil

et al. 2012

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Two hydrocarbon-biodegrading bacterial strains, B1 and B2, were isolated from petroleum-contaminated soil collected from Tianjin, China. The strains were identified as Pseudomonas aeruginosa (B1) and Acinetobacter junii (B2). The degradation rate of n-hexadecane by B1 and B2 reached 96% and 78% respectively after 7 days, though the strains employed different mechanisms of degradation. The results showed that B2 was not able to use glucose as carbon source. B1 could produce glycolipid surfactants using glucose as the carbon source, according to the results of blue agar plate analysis and thin layer chromatography (TLC), and the bacterial culture of B1 had a high oil discharge and emulsification activity. Both B1 and B2 could produce biosurfactants with hexadecane as the sole carbon source, but their modes of action were different. The carbon source was found to affect the cell surface hydrophobicity. Cell surface hydrophobicity was poor with glucose as the carbon source, but enhanced when hexadecane was used as the carbon source.

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Section: Discussionmentioning

confidence: 99%

Biodegradation of n-hexadecane by bacterial strains B1 and B2 isolated from petroleum-contaminated soil

et al. 2012

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“…Moreover, a variety of different hydrophobic substrates were efficiently emulsified by P. aeruginosa strain KVD-HR42 supernatant. It was reported in a study that nhexadecane was attracted to the cell surface of the 21 BN strain of P. putida at a rate of 72% (Tuleva et al, 2002). In a different study by Noordman and Janssen (2002), it was found that n-hexadecane was attracted to the cell surfaces of the strains of biosurfactant producer P. aeruginosa 18 UG2, Acinetobacter calcoaceticus RAG1, Rhodococcus erythropolis DSM 43066, and Rhodococcus erythropolis ATCC 19558 at rates of 42, 81, 30, and 12%, respectively.…”

Section: Emulsification Activitymentioning

confidence: 99%

Rhamnolipid biosurfactant production by Pseudomonas aeruginosa strain KVD-HR42 isolated from oil contaminated mangrove sediments

Deepika¹,

Raghuram²,

Bramhachari³

2017

Afr. J. Microbiol. Res.

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Pseudomonas aeruginosa strain KVD-HR42 exhibiting growth and biosurfactant production with 1% molasses as the sole carbon source was isolated from oil contaminated mangrove sediments. Optimization of media conditions involving variations in carbon, nitrogen sources, amino acids, pH, temperature, and NaCl% were evaluated with the aim of increasing biosurfactant productivity and surface tension reduction (STR). The highest biosurfactant production of 4.83 g/L was obtained when cells were grown in mineral salts media (MSM) supplemented with 1% (w/v) molasses, NaNO 3 , and leucine 0.1% (w/v) at 35±2°C at 150 rpm after 48 h. The results obtained from kinetics study indicated that biosurfactant production, E 24 %, and rhamnose concentration were growth associated. However, maximum biosurfactant production occurred in the exponential growth phase and detected an increase in E 24 % and rhamnose concentration. The Fourier transform infrared (FTIR) spectra confirmed the rhamnolipid nature of the biosurfactant. Stability studies revealed the thermostable activity of biosurfactant (110°C for 15 min) and could also withstand wide pH and NaCl ranges. Maximum oil biodegradation of 68% was achieved with 1% waste lubricant oil (WLO). The biosurfactant emulsified various hydrocarbons with varied efficiencies. However maximum E 24 % and E 48 % activity was exhibited with n-hexadecane (69.5 and 40%). The results reveal the potential of strain KVD-HR42 biosurfactant for the bioremediation of petroleum hydrocarbons in mangrove sediments.

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“…The cell surfaces of the strains attracted benzene at a rate of 73% and 62%, n-hexane at a rate of 40% and 29%, toluene at a rate of 65% and 72%, and xylene at a rate of 60% and 53%, respectively (Table 4). It was reported in another study that n-hexadecane was attracted to the cell surface of the 21 BN strain of P. putida at a rate of 72% (Tuleva et al, 2002). In a study by Noordman and Janssen (2002) at rates of 42%, 81%, 30%, and 12%, respectively.…”

Section: Emulsification Activity and Cell Surface Hydrophobicitymentioning

confidence: 92%

Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey

Kaya¹,

Aslım²,

Kariptaş³

2014

Turk J Biol

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In this study, 26 Pseudomonas spp. were isolated from a stream polluted by factory waste and from petroleum-contaminated soil. The surface tension (ST) of the cultures was used as a criterion for the primary isolation of biosurfactant-producing bacteria. Biosurfactant production was quantified by ST reduction, critical micelle concentration (CMC), emulsification capacity (EC), and cell surface hydrophobicity (CSH). Two of the isolates, P. aeruginosa 78 and 99, produced rhamnolipid biosurfactant. The strains started rhamnolipid production in the logarithmic phase. They decreased the ST of the culture from 73 dyne/cm 2 to 29 and 33 dyne/cm 2 , and the CMC of produced rhamnolipids were 115 and 130 mg/L, respectively. P. aeruginosa 78 and 99 strains emulsified benzene and n-hexane at the highest rates, and the surfaces of these strains were 73% and 65% and 62% and 72% more hydrophobic for benzene and toluene, respectively.

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Biosurfactant Production By A New Pseudomonas Putida Strain

Cited by 138 publications

References 2 publications

Biodegradation of n-hexadecane by bacterial strains B1 and B2 isolated from petroleum-contaminated soil

Biodegradation of n-hexadecane by bacterial strains B1 and B2 isolated from petroleum-contaminated soil

Rhamnolipid biosurfactant production by Pseudomonas aeruginosa strain KVD-HR42 isolated from oil contaminated mangrove sediments

Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey

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