Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition

Abstract

Biosurfactant production was screened in four lactobacilli strains. The highest biosurfactant production (excreted and cell-bound biosurfactants) was achieved withLactobacillus paracaseissp.paracaseiA20, a strain isolated from a Portuguese dairy plant, with a decrease in the surface tension of 6.4 mN m−1and 22.0 mN m−1, respectively. Biosurfactant production by this strain was evaluated under different culture broth compositions. The use of different nitrogen sources revealed that yeast extract is essential for bacterial growth, while peptone is crucial for biosurfactant synthesis. For biosurfactant production, the use of peptone and meat extract yielded a higher production when compared to the standard medium, with a surface tension reduction of 24.5 mN m−1Furthermore, experiments were also conducted in a reactor with pH and temperature control. Biomass and biosurfactant production in bioreactor was higher comparing with the experiments conducted in shake flaks. The optimization procedure adopted in the current work was found to improve the biosurfactant production and opened new perspectives for the use ofL. paracaseissp.paracaseiA20 as a promising biosurfactant-producer.

Biosurfactant/s from Lactobacilli species: Properties, challenges and potential biomedical applications

“…Detailed studies on several mechanisms of interference of pathogen adherence and biosurfactant have been previously reported by several researchers . The mechanism of competitive exclusion has been demonstrated by quite a lot of Lactobacilli spp.…”

Antifungal Screening of Bioprotective Isolates against Botrytis cinerea, Fusarium pallidoroseum and Fusarium moniliforme

“…Biosurfactants produced by bacteria can either be excreted or remain cell-bound. Gudiña et al [63] found that Lactobacillus coryniformis, Lactobacillus paracasei, L. plantarum and Leuconostoc mesenteroides produced cell-bound biosurfactants at higher levels than excreted ones. Cell-bound biosurfactant production has been observed in several species of Lactobacillus [64], including L. plantarum [65].…”

Synergistic effects of reduced graphene oxide and hydroxyapatite on osteogenic differentiation of MC3T3-E1 preosteoblasts

“…The data is expressed as mean ± SD based on at least duplicate observations from three independent experiments. important factors influencing the cellular behaviors and the increase in pH value can lead to the inhibition of cell proliferation [45,46]. A colloidal dispersion of HAp microparticles at 10 mg/mL raised the pH of the culture media up to 7.76 and 7.97 (from 7.32) after 24 h and 1 wk of incubation at 37 C, respectively (Table.…”