Biosurfactant mannosyl-erythritol lipid inhibits secretion of inflammatory mediators from RBL-2H3 cells

Morita, Yoshifumi; Tadokoro, Satoshı; Sasai, Masao; Kitamoto, Daï; Hirashima, Naohide

doi:10.1016/j.bbagen.2011.07.002

Cited by 33 publications

(25 citation statements)

References 43 publications

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“…23 L-carnitine, erythritol, and/or betaine have been reported to serve as osmoprotectants for hyperosmotic stress 20,23,51 and inflammatory conditions. 51,52 L-carnitine therapy has been shown considerable promise in addressing a variety of disease states that involve both primary and secondary carnitine deficiency, cardiovascular disorders, and other diseases. 53–57 …”

Section: Discussionmentioning

confidence: 99%

Effects ofl-Carnitine, Erythritol and Betaine on Pro-inflammatory Markers in Primary Human Corneal Epithelial Cells Exposed to Hyperosmotic Stress

Hua

Deng

et al. 2014

Current Eye Research

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Purpose To explore the effects of osmoprotectants on pro-inflammatory mediator production in primary human corneal epithelial cells (HCECs) exposed to hyperosmotic stress. Methods HCECs cultured in iso-osmolar medium (312mOsM) were switched to hyperosmotic media with or without prior incubation with 2–20mM of L-carnitine, erythritol or betaine for different time periods. The mRNA expression and protein production of pro-inflammatory markers in HCECs were evaluated by RT-qPCR and ELISA. Results Hyperosmolar media significantly stimulated the mRNA and protein expression of pro-inflammatory cytokines, TNF-α, IL-1β and IL-6, and chemokines, IL-8, CCL2 and CCL20 in HCECs in an osmolarity dependent manner. The stimulated expression of these pro-inflammatory mediators was significantly but differentially suppressed by L-carnitine, erythritol or betaine. L-carnitine displayed the greatest inhibitory effects and down-regulated 54–77% of the stimulated mRNA levels of TNF-α (down from 12.3 to 5.7 fold), IL-1β (2.2 to 0.9 fold), IL-6 (7.3 to 2.9 fold), IL-8 (4.6 to 2.0 fold), CCL2 (15.3 to 3.5 fold) and CCL20 (4.1 to 1.5 fold) in HCECs exposed to 450mOsM. The stimulated protein production of TNF-α, IL-1β, IL-6 and IL-8 was also significantly suppressed by L-carnitine, erythritol and betaine. L-carnitine suppressed 49–79% of the stimulated protein levels of TNF-α (down from 81.3 to 17.4pg/ml), IL-1β (56.9 to 29.2pg/ml), IL-6 (12.8 to 4.6ng/ml), and IL-8 (21.2 to 10.9ng/ml) by HCECs exposed to 450mOsM. Interestingly, hyperosmolarity stimulated increase in mRNA and protein levels of TNF-α, IL-1β and IL-6 were significantly suppressed by a TRPV1 activation inhibitor capsazepine. Conclusions L-carnitine, erythritol and betaine function as osmoprotectants to suppress inflammatory responses via TRPV1 pathway in HCECs exposed to hyperosmotic stress. Osmoprotectants may have efficacy in reducing innate inflammation in dry eye disease.

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Section: Discussionmentioning

confidence: 99%

Effects ofl-Carnitine, Erythritol and Betaine on Pro-inflammatory Markers in Primary Human Corneal Epithelial Cells Exposed to Hyperosmotic Stress

Hua

Deng

et al. 2014

Current Eye Research

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“…From this work, we have been able to dramatically reduce the production cost of MELs through the use of an improved fermentation process, and the identification of fungal strains capable of using raw sugarcane juice as the main carbon source. Further decreases in the overall production cost will be necessary for the expanded use of MELs outside of the cosmetics industry, as described previously 5,13 . Additional investigations regarding the production, biosynthesis, and physicochemical properties of these functional glycolipids will be necessary to fully exploit MELs in commercial applications.…”

Section: Further Perspectivesmentioning

confidence: 99%

“…these compounds hold tremendous promise for use in a wide range of applications, including food production, cosmetics, and pharmaceuticals 5,13 .…”

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confidence: 99%

Mannosylerythritol Lipids: Production and Applications

et al. 2015

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“…To estimate syntaxin‐3 phosphorylation levels, phosphorylated proteins were collected using the PhosphoProtein purification kit, which is often used to detect phosphorylated SNARE proteins in mast cells. Some researches revealed that phosphorylation levels of SNAP‐23, syntaxin‐4 and VAMP‐8 but not syntaxin‐3 increase after stimulation using this kit (Nanamori et al, ; Hiramatsu et al, ; Morita et al, ; Inoh et al, ). The overexpression of wild‐type syntaxin‐3 in RBL‐2H3 cells increased the levels of phosphorylated syntaxin‐3 compared with those in mock‐transfected cells (Figure B).…”

Section: Resultsmentioning

confidence: 96%

Phosphorylation of syntaxin‐3 at Thr 14 negatively regulates exocytosis in RBL‐2H3 mast cells

Tadokoro

Shibata

Inoh

et al. 2016

Cell Biology International

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Recent studies have revealed that soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins interact with each other, forming a SNARE complex that induces exocytosis in mast cells. Previously, we reported that syntaxin-3, a SNARE protein, regulates mast cell exocytosis and is constantly phosphorylated. In this study, we tried to identify the amino acid residue that is phosphorylated in mast cells, and to elucidate the regulatory mechanism of exocytosis by phosphorylation in syntaxin-3. We found that Thr 14 of syntaxin-3 was a phosphorylation site in mast cells. In addition, the overexpression of a constitutively dephosphorylated syntaxin-3 (T14A) mutant enhanced mast cell exocytosis. We also showed that the phosphomimetic mutation of syntaxin-3 at Thr 14 (T14E) induced structural changes in syntaxin-3, and this mutation inhibited binding of syntaxin-3 to Munc18-2. These results suggest that phosphorylated syntaxin-3 at Thr 14 negatively regulates mast cell exocytosis by impairing the interaction between syntaxin-3 and Munc18-2.

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Biosurfactant mannosyl-erythritol lipid inhibits secretion of inflammatory mediators from RBL-2H3 cells

Cited by 33 publications

References 43 publications

Effects ofl-Carnitine, Erythritol and Betaine on Pro-inflammatory Markers in Primary Human Corneal Epithelial Cells Exposed to Hyperosmotic Stress

Effects ofl-Carnitine, Erythritol and Betaine on Pro-inflammatory Markers in Primary Human Corneal Epithelial Cells Exposed to Hyperosmotic Stress

Mannosylerythritol Lipids: Production and Applications

Phosphorylation of syntaxin‐3 at Thr 14 negatively regulates exocytosis in RBL‐2H3 mast cells

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