Biosurfactant coated silver and iron oxide nanoparticles with enhanced anti-biofilm and anti-adhesive properties

Khalid, Hafiza F.; Tehseen, Bushra; Sarwar, Yasra; Hussain, Syed Zajif; Khan, Waheed S.; Raza, Zulfiqar Ali; Bajwa, Sadia Z.; Kanaras, Antonios G.; Hussain, Irshad; Rehman, Asma

doi:10.1016/j.jhazmat.2018.10.049

Cited by 91 publications

(54 citation statements)

References 39 publications

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“…These findings can be explained based on the disruptive power of rhamnolipid on biofilm matrix, the characteristic antibacterial properties of clarithromycin and chitosan NPs, the preventive actions of chitosan NPs and rhamnolipids on bacteria adhesion, and biofilm formation. Similarly, rhamnolipid-coated metallic nanoparticles (silver and iron oxide) were fabricated by Khalid et al [175] and demonstrated excellent anti-biofilm efficacy against S. aureus and P. aeruginosa biofilms. Diagrammatic illustration of the novel approaches utilized for combating antibacterial resistance.…”

Section: Lipid Polymer Nanoparticles (Lpns)mentioning

confidence: 99%

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

et al. 2020

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Based on the recent reports of World Health Organization, increased antibiotic resistance prevalence among bacteria represents the greatest challenge to human health. In addition, the poor solubility, stability, and side effects that lead to inefficiency of the current antibacterial therapy prompted the researchers to explore new innovative strategies to overcome such resilient microbes. Hence, novel antibiotic delivery systems are in high demand. Nanotechnology has attracted considerable interest due to their favored physicochemical properties, drug targeting efficiency, enhanced uptake, and biodistribution. The present review focuses on the recent applications of organic (liposomes, lipid-based nanoparticles, polymeric micelles, and polymeric nanoparticles), and inorganic (silver, silica, magnetic, zinc oxide (ZnO), cobalt, selenium, and cadmium) nanosystems in the domain of antibacterial delivery. We provide a concise description of the characteristics of each system that render it suitable as an antibacterial delivery agent. We also highlight the recent promising innovations used to overcome antibacterial resistance, including the use of lipid polymer nanoparticles, nonlamellar liquid crystalline nanoparticles, anti-microbial oligonucleotides, smart responsive materials, cationic peptides, and natural compounds. We further discuss the applications of antimicrobial photodynamic therapy, combination drug therapy, nano antibiotic strategy, and phage therapy, and their impact on evading antibacterial resistance. Finally, we report on the formulations that made their way towards clinical application.

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Section: Lipid Polymer Nanoparticles (Lpns)mentioning

confidence: 99%

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

et al. 2020

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“…This caused a hydrophobic phospholipid collapse in the cell membrane, leading to pore formation and bacterial cell death [ 63 ] ( Figure 5 A,B). In another report, Khalid et al used rhamnolipid-coated AgNPs and proved their antimicrobial activity in early and mature biofilms of S. aureus and P. aeruginosa [ 68 ]. Rhamnolipids have a tendency to interact with the bacterial cell membrane and biofilm lipids via hydrophobic interactions.…”

Section: Interaction Of Gold and Silver Nanoparticles With Biofilmmentioning

confidence: 99%

Interactions of Gold and Silver Nanoparticles with Bacterial Biofilms: Molecular Interactions behind Inhibition and Resistance

Joshi

Singh

Mijaković

2020

IJMS

140

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Many bacteria have the capability to form a three-dimensional, strongly adherent network called ‘biofilm’. Biofilms provide adherence, resourcing nutrients and offer protection to bacterial cells. They are involved in pathogenesis, disease progression and resistance to almost all classical antibiotics. The need for new antimicrobial therapies has led to exploring applications of gold and silver nanoparticles against bacterial biofilms. These nanoparticles and their respective ions exert antimicrobial action by damaging the biofilm structure, biofilm components and hampering bacterial metabolism via various mechanisms. While exerting the antimicrobial activity, these nanoparticles approach the biofilm, penetrate it, migrate internally and interact with key components of biofilm such as polysaccharides, proteins, nucleic acids and lipids via electrostatic, hydrophobic, hydrogen-bonding, Van der Waals and ionic interactions. Few bacterial biofilms also show resistance to these nanoparticles through similar interactions. The nature of these interactions and overall antimicrobial effect depend on the physicochemical properties of biofilm and nanoparticles. Hence, study of these interactions and participating molecular players is of prime importance, with which one can modulate properties of nanoparticles to get maximal antibacterial effects against a wide spectrum of bacterial pathogens. This article provides a comprehensive review of research specifically directed to understand the molecular interactions of gold and silver nanoparticles with various bacterial biofilms.

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“…20 The MIC of CEN-ZnO NPs was determined against Gram-positive (S. aureus ATCC 25923) and Gram-negative bacteria (P. aeruginosa PAO1 and E. coli 25922) by 96-well microtiter assay. Briey, 100 ml of Mueller-Hinton broth with CEN-ZnO NPs at different concentrations (i.e., 4,8,16,32,64,128,256,512, 1000, and 2500 mg ml À1 ) was added. Then, 5 ml of microbial suspension (1 Â 10 8 CFU ml À1 ) was added to each well.…”

Section: Evaluation Of Antibacterial Activity Of Biosynthesized Cen-zmentioning

confidence: 99%

“…6,7 The presence of bioactive compounds in plants, algae, and microorganisms has made them a good candidate for the synthesis of nanoparticles (NPs). 8 Cyanobacteria as a group of ancient and abundant microorganisms with prokaryotic cell structures have the capability of carbon dioxide-dependent photosynthesis. 9 Among microorganisms, cyanobacteria are of great interest in NP synthesis, as they are a potential source of novel metabolites that have great importance from a biotechnological and industrial point of view.…”

Section: Introductionmentioning

confidence: 99%

A bio-inspired strategy for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the cell extract of cyanobacterium Nostoc sp. EA03: from biological function to toxicity evaluation

et al. 2019

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Cyanobacteria, as one of the largest groups of phototrophic bacteria, have a high potential as an excellent source of fine chemicals and bioactive compounds, including lipid-like compounds, amino acid derivatives, proteins, and pigments. This study aimed to synthesize ZnO nanoparticles using the cell extract of the cyanobacterium Nostoc sp. EA03 (CEN-ZnO NPs) through a rapid and eco-friendly approach. The biosynthesized nanoparticles, CEN-ZnO NPs, were characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), zeta potential measurement, differential scanning calorimetry (DSC)/thermogravimetric analysis (TGA), FTIR, SEM, TEM, and EDX spectroscopy. The UV-Vis spectrum showed an absorption peak at 370 nm. The star-shaped CEN-ZnO NPs, as observed in the TEM and SEM images, had an average diameter of 50-80 nm. MIC and MBC values for E. coli, P. aeruginosa and S. aureus, were determined to be, respectively, 2000, 2000, and 64 mg ml À1 , and 2500, 2500 and 128 mg ml À1 . Further analysis through confocal laser scanning microscopy (CLSM) provided the observable confirmation that the CEN-ZnO NPs stunted the bacterial growth, preventing the formation of exopolysaccharides. The AFM analysis of surface topography of bacterial biofilm samples treated with CEN-ZnO NPs showed a rugged topography in some parts of the biofilm surface, indicating the destruction of biofilms. In contrast, in the untreated control samples, the structured biofilms were flat and prominent. MTT assay indicated that CEN-ZnO NPs had less cytotoxicity on the MRC-5 lung fibroblast cells compared with the cancerous treated A549 cells. As the concentration of the CEN-ZnO NPs increased, the amount of ROS produced in the tested bacterial strains also increased. Analyzing the data obtained from flow cytometry showed that the higher concentrations of CEN-ZnO NPs lead to a reduction in the viability of P. aeruginosa PAO1, E. coli and S. aureus. The biosynthesized ZnO nanoparticles using Nostoc cell extracts exhibited different attributes, inspiring enough to be considered for further investigation.

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Biosurfactant coated silver and iron oxide nanoparticles with enhanced anti-biofilm and anti-adhesive properties

Cited by 91 publications

References 39 publications

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

Interactions of Gold and Silver Nanoparticles with Bacterial Biofilms: Molecular Interactions behind Inhibition and Resistance

A bio-inspired strategy for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the cell extract of cyanobacterium Nostoc sp. EA03: from biological function to toxicity evaluation

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