Biosurfactant and enzyme mediated crude oil degradation by Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3

Abstract

The present study focuses on the optimization of biosurfactant (BS) production using two potential biosurfactant producer NA3 and MN3 and role of enzymes in the biodegradation of crude oil. The optimal conditions for NA3 and MN3 for biodegradation were pH of 8 and 7; temperature of 30 and 40 °C, respectively. NA3 and MN3 produced 3.81 and 4.68 g/L of BS, respectively. Gas chromatography mass spectrometry confirmed that BS was mainly composed of fatty acids. Furthermore, the role of the degradative enzymes, alkane hydroxylase, alcohol dehydrogenase and laccase on biodegradation of crude oil are explained. Maximum biodegradation efficiency (BE) was recorded for mixed consortia (86%) followed by strain NA3 (84%). Both bacterial strains were found to be vigorous biodegraders of crude oil than other biosurfactant-producing bacteria due to their enzyme production capabilities and our results suggests that the bacterial isolates can be used for effective degradation of crude oil within short time periods.

Novel Bionanocompounds: Outer Membrane Protein A and Laccase Co-Immobilized on Magnetite Nanoparticles for Produced Water Treatment

“…Table 2 summarizes the peaks where major changes in composition were observed, as evidenced by a reduction in the area under the curve. Additionally, we estimated the removal efficiencies (RE(%)) for the relevant compounds by following the relation reported by Parthipan et al [ 24 ]: RE (%) = 100 − (As × 100/Ac), where As is the total area of peaks in every sample and Ac is the total area of peaks in the reference sample. Except for nonadecane, the removal efficiencies of the involved compounds were the highest for the MNP-PEA-OmpA-Laccase bionanocompound.…”

“…Traditional methods for emulsified wastewater treatment are based on mechanical (ultrafiltration) [ 21 ], physical-chemical (photocatalysis) [ 22 ], or biological (bioremediation) approaches [ 23 ]. The last group of processes has attracted significant attention and mainly refers to the incorporation of active biomolecules or whole microorganisms into different stages during the treatment of the recovered effluents [ 24 ]. This approach is advantageous due the low toxicity and environmental friendliness of these agents [ 25 ].…”

“…This approach is advantageous due the low toxicity and environmental friendliness of these agents [ 25 ]. One of the most useful groups of biomolecules for emulsion bioremediation (due to their interfacial activity) are biosurfactants, which have been categorized into different families including lipids, proteins, and bacteria [ 24 , 25 , 26 , 27 , 28 ]. Despite the possible benefits, biosurfactants tend to face stability issues that compromise their performance, especially when subjected to operating conditions far from those observed in their native environments [ 29 ].…”

Biofilm formation on copper and its control by inhibitor/biocide in cooling water environment

“…Results obtained were then modelled and simulated using the EQUVRT software and two equivalent circuit models were used for fitting the data such as R(QR) and R(Q(R(QR))) respectively. Tafel plots and potentiodynamic scanning curves were measured with a scan rate of 0.5 mV/s ( Parthipan et al 2017a )…”

Characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria: potential application in bioremediation

“…Morphologically different bacterial colonies thus obtained were further purified on MSM agar plates with or without 0.1 % (v/v) n-hexadecane to eliminate autotrophs and agarutilizing bacteria. The procedure was repeated and isolates showing pronounced growth on n-hexadecane were selected and preserved for further characterization (Parthipan et al 2017). Screening for biosurfactant production by the selected strains was done using de novo ring method, qualitative drop-collapse test and oil displacement test (Bodour and Maier 1998).…”