Biostimulation combined treatments for remediation of diesel contaminated soil

Abstract

Bioremediation is an important technology for the restoration of oil polluted environments by indigenous or selected microorganisms. In general, the rate of biodegradation depends on the number and types of microorganisms, the nature and chemical structure of pollutants to be degraded and the environmental conditions. In this study we have evaluated the efficacy of the application of four different biostimulation treatments for the biodegradation of diesel contaminated soils. The treatments applied involved: (a) the addition of NPK fertilizer + Ivey I surfactant; (b) the addition of NPK fertilizer + Ethanol; (c) the addition of NPK fertilizer + Biorem; and (d) oxidation by Fenton's reagent combined with NPK fertilizer. Microbial activity was evaluated following growth of heterotrophic and degrading microorganisms, dehydrogenase activity and CO 2 production. Hydrocarbons degradation was established by determination of TPH, alkanes, branched alkanes, pristane and phytane by GC/MS. Our results have shown that the application of NPK fertilizer in combination with Ivey surfactant is an efficient treatment to be applied in clay soil. Treatment with Fenton's reagent previous to the application of NPK fertilizer also efficiently enhanced hydrocarbon biodegradation in saturated conditions.

Ecological Response in the Integrated Process of Biostimulation and Bioaugmentation of Diesel-Contaminated Soil

“…By contrast, the amount of alkane degradation bacteria (ADB) and naphthalene degradation bacteria (NDB) increased. This indicated diesel contaminants harmful to UB, as described by Calvo et al [51].…”

A novel system coupling an electro-Fenton process and an advanced biological process to remove a pharmaceutical compound, metronidazole

“…[55]. Different studies also showed the efficacy of using biostimulation as a post-treatment after oxidation with Fenton's reagents for the treatment of hydrocarbons [56,57].…”