Biostabilization of a Sandy Soil Using Enzymatic Calcium Carbonate Precipitation

Carmona, João P. S. F.; Oliveira, Paulo J. Venda; Lemos, Luís

doi:10.1016/j.proeng.2016.06.144

Cited by 59 publications

(28 citation statements)

References 19 publications

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“…Consequently, the bio-stabilization process is less effective. This is consistent with the views of other authors [34,35] who recorded that high CaCl 2 -urea concentration is related to the amount of urease and may restrain the activity of urease, which may in turn result in a reduction in calcium carbonate precipitation. Also, the results showed that the amount of precipitated CaCO 3 increased as the concentration of bacteria (1.5 -3 mL) increased, as shown in the test tubes 3 to 6.…”

Section: Effect Of Bfc Concentrations On the Stabilization Of Siliceous Sand Soilsupporting

confidence: 93%

Stabilization Characteristics of Different Loose Sandy Soils using Microbial-Induced Calcite Precipitation

Mohamed

Hafez

El-Mahllawy

et al. 2021

Walailak J Sci & Tech

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Loose sands (siliceous, silty, and calcareous classes) are extensively found near arid areas in Egypt. Furthermore, many geotechnical structures, like water channels and roads, may be constructed on weak or loose sand soils. The geotechnical behavior of loose sands is usually connected with different interdependent problems, such as high permeability, low shear strength, low bearing capacity, high seepage, and low stability. This work characterized the effect of stabilization of the siliceous, silty, and calcareous sandy soils via biocementation process using Sporosarcina pasteurii bacteria as a potential eco, commercial, and engineering solution. This was carried out using bacteria, fixation, and cementation solutions (BFC) at different times number additions. The results indicated that the addition times of solution have a remarkable effect on the physical and mechanical properties of sandy soils. The results also proved that the precipitation of calcite by the bacterial activity led to cohesion of soil grains, and this increased the resistance of soils to deterioration. In addition, the high content of the precipitated calcium carbonate enhanced the shear strength and the unconfined compressive strength and decreased the soil permeability. S. pasteurii bacteria can be used successfully and commercially in the biocementation process for siliceous sand, silty sand, and calcareous sandy soils in Egypt using the recommended conditions and mixes.

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Section: Effect Of Bfc Concentrations On the Stabilization Of Siliceous Sand Soilsupporting

confidence: 93%

Stabilization Characteristics of Different Loose Sandy Soils using Microbial-Induced Calcite Precipitation

Mohamed

Hafez

El-Mahllawy

et al. 2021

Walailak J Sci & Tech

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“…Many researchers have however, reported the improvement of hardness and the reduction of soil permeability using bacteria with urease activity (Carmona, Oliveira & Lemos, 2016;Chu, Stabnikov & Ivanov, 2012;DeJong, Fritzges & Nüsslein, 2006;Ferris et al, 1996;Whiffin et al, 2007).…”

Section: Soil and Sand Bioconsolidationmentioning

confidence: 99%

Soil bacteria that precipitate calcium carbonate: mechanism and applications of the process

Acuña¹,

Becerra²,

Martínez³

et al. 2018

Acta Agron.

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Bacteria with ureasic activity are microorganisms found in soil that in presence of urea and calcium, they can produce calcium carbonate, a process known as microbiologically induced calcium carbonate precipitation (MICP). This article discusses this process and its mechanism, as well as bacterial urease, calcium carbonate crystals formed, and factors that affect the efficiency of MICP, as type of bacteria, bacterial cell concentrations, pH, temperature and calcium and urea concentrations. In addition, applications as removal of heavy metals in water, bioconsolidation, biocement and CO 2 sequestration are also discussed.Keywords: Biomineralization; Bacillus; urease; carbonates; heavy metals; bioconsolidation; biocementation. ResumenLas bacterias con actividad ureásica son microorganismos que se encuentran en el suelo, y que en presencia de urea y calcio, pueden producir carbonato de calcio, proceso conocido como precipitación de calcio inducida microbiológicamente (PCIM). Este artículo trata este proceso y su mecanismo, además de las ureasas de origen bacteriano, los cristales de carbonato de calcio formado, los factores que afectan la eficiencia la PCIM, como el tipo de bacteria, las concentraciones de células bacterianas, el pH, la temperatura y las concentraciones de calcio y urea. Además, se incluye las aplicaciones como la remoción de metales pesados en aguas, la bioconsolidación, biocemento y secuestro de CO 2 .

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“…Several processes have been found in which CaCO 3 is formed by bacteria, including urea hydrolysis, denitrification, sulfate reduction, photosynthesis, and methane oxidation [ 6 , 7 ]. Among them, urea hydrolysis is the most studied and widely applied because of its excellent CaCO 3 formation efficiency [ 8 , 9 ]. To date, most investigations on BICP by ureolytic bacteria have been conducted using the urease-producing strain of Sporosarcina pasteurii [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Novel CaCO3-Forming Alkali-Tolerant Rhodococcus erythreus S26 as a Filling Agent for Repairing Concrete Cracks

Choi

Park

et al. 2021

Molecules

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Biomineralization, a well-known natural phenomenon associated with various microbial species, is being studied to protect and strengthen building materials such as concrete. We characterized Rhodococcus erythreus S26, a novel urease-producing bacterium exhibiting CaCO3-forming activity, and investigated its ability in repairing concrete cracks for the development of environment-friendly sealants. Strain S26 grown in solid medium formed spherical and polygonal CaCO3 crystals. The S26 cells grown in a urea-containing liquid medium caused culture fluid alkalinization and increased CaCO3 levels, indicating that ureolysis was responsible for CaCO3 formation. Urease activity and CaCO3 formation increased with incubation time, reaching a maximum of 2054 U/min/mL and 3.83 g/L, respectively, at day four. The maximum CaCO3 formation was achieved when calcium lactate was used as the calcium source, followed by calcium gluconate. Although cell growth was observed after the induction period at pH 10.5, strain S26 could grow at a wide range of pH 4–10.5, showing its high alkali tolerance. FESEM showed rhombohedral crystals of 20–60 µm in size. EDX analysis indicated the presence of calcium, carbon, and oxygen in the crystals. XRD confirmed these crystals as CaCO3 containing calcite and vaterite. Furthermore, R. erythreus S26 successfully repaired the artificially induced large cracks of 0.4–0.6 mm width.

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Biostabilization of a Sandy Soil Using Enzymatic Calcium Carbonate Precipitation

Cited by 59 publications

References 19 publications

Stabilization Characteristics of Different Loose Sandy Soils using Microbial-Induced Calcite Precipitation

Stabilization Characteristics of Different Loose Sandy Soils using Microbial-Induced Calcite Precipitation

Soil bacteria that precipitate calcium carbonate: mechanism and applications of the process

Characterization of a Novel CaCO3-Forming Alkali-Tolerant Rhodococcus erythreus S26 as a Filling Agent for Repairing Concrete Cracks

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