Biospecific isolation and characterization of angiogenesis-promoting ingredients in Buyang Huanwu decoction using affinity chromatography on rat brain microvascular endothelial cells combined with solid-phase extraction, and HPLC-MS/MS

Liao, Fengyun; Meng, Yingjiao; Zheng, Huazhu; Dan, He; Shen, Xiao; Yu, Jianye; Wu, Yingai; Wang, Lisheng

doi:10.1016/j.talanta.2017.11.018

Cited by 33 publications

(16 citation statements)

References 41 publications

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“…The potential interference of endogenous dialysate matrix components was investigated at the retention times of all analytes and IS by analyzing the response of the blank dialysate, blank dialysate spiked with IS at LLOQ and dialysate samples obtained 2 h after administration. 27 …”

Section: Experimental Methods and Materialsmentioning

confidence: 99%

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<p>Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS</p>

Zheng¹,

Shen²,

He³

et al. 2020

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The aim of our research was to analyze and compare the pharmacokinetics of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in the blood and brain tissue of normal and cerebral ischemia-reperfusion injury rats by HPLC-MS/MS method. Methods: The blood and brain tissue samples of normal and middle cerebral artery occlusion (MCAO) rats were compared. The blood and brain tissue samples were collected by using microdialysis technique. The concentrations of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in blood and brain tissues were determined by the HPLC-MS/MS internal standard method. Results: Compared with the normal group, the model group after the administration of the Huangqi Guizhi Wuwu Decoction showed that C max blood , AUC 0-t blood , and AUC 0-inf blood of paeoniflorin were increased, CL blood , t 1/2 brain , and V brain of paeoniflorin were decreased; C maxblood , AUC 0-tblood , AUC 0-infblood , and average residence time (MRT brain) of calycosin-7-o-β-d-6-glucoside were decreased and the CL blood and C max brain of calycosin-7-o-βd-6-glucoside were increased; C max blood of calycosin was decreased, V blood and V brain of calycosin were increased; C max blood , AUC 0-t blood , AUC 0-inf blood , and MRT brain of 6-gingerol were decreased, CL blood of 6-gingerol was increased. Conclusion: This method is simple, rapid, and sensitive. It is suitable for the pharmacokinetic study of Huangqi Guizhi Wuwu Decoction in the blood and brain tissue of rats. Cerebral ischemia-reperfusion injury increased the content of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in the blood, affecting the clearance rate of paeoniflorin in the brain, the detention time of calycosin-7-o-β-d-6-glucoside and the 6-gingerol in the brain. In normal and cerebral ischemia-reperfusion rats, the content of paeoniflorin and 6-gingerol in the blood was higher than that in brain tissue, while the content of calycosin, calycosin-7-o-β-d-6-glucoside in the brain tissue was higher than that in blood, suggesting that calycosin and calycosin-7-o-β-d-6-glucoside have brain targeting properties.

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Section: Experimental Methods and Materialsmentioning

confidence: 99%

“…Development and Therapy downloaded from https://www.dovepress.com/ by 44.224.250.200 on 03-Nov-2020 For personal use only.times of all analytes and IS by analyzing the response of the blank dialysate, blank dialysate spiked with IS at LLOQ and dialysate samples obtained 2 h after administration 27. …”

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<p>Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS</p>

Zheng¹,

Shen²,

He³

et al. 2020

DDDT

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“…BHD (111 g) was composed of Radix Astragali, Semen Persicae, Radix Angelicae Sinensis, Flos Carthami, Rhizoma Ligustici Chuanxiong, Radix Paeoniae Rubra, and Lumbricus at a 60 : 9 : 9 : 9 : 6 : 9 : 9 ratio. The extraction and preparation process of BHD was according to our previous study [19]. In short, the herbs were soaked in water for 0.5 h, and then extracted twice in a water bath, concentrated under reduced pressure, and was purified using D101 macroporous resin to collect 40% and 70% ethanol eluent.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, sample solution incubates cells, and washing solution elutes unbound components; then cells are incubated with dissociation solution; the targets on the cell membrane are inactivated, collecting the dissociation liquid; and the target components are confirmed by HPLC-MS/MS. In the previous study, we successfully screened the angiogenesis-promoting ingredients in BHD by brain microvascular endothelial cells and screened the antithrombotic components by platelets [19, 20]. Accordingly, we attempted to screen the active ingredients that promote axonal regeneration in BHD by using PC12 cells.…”

Section: Introductionmentioning

confidence: 99%

Cell Chromatography-Based Screening of the Active Components in Buyang Huanwu Decoction Promoting Axonal Regeneration

Yan

Wang

et al. 2019

BioMed Research International

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Buyang Huanwu decoction (BHD), a popular formulation prescribed in traditional Chinese medicine (TCM) for the treatment of ischemic stroke, has been reported to have a potential role in promoting axonal regeneration. The purpose of the study was to screen and identify bioactive compounds from BHD using live PC12 cells coupled with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Using this approach, we successfully identified six bioactive components from BHD. These components have protective effects on oxygen-glucose deprivation/reperfusion (OGD/R) injury to PC12 cells. Furthermore, calycosin-7-d-glucoside (CG) and formononetin-7-O-β-d-glucoside (FG) could upregulate the protein expression of growth-associated protein 43 (GAP-43) and brain-derived neurotrophic factor (BDNF). This study suggests that living cells combined with HPLC-MS/MS can be used for the screening of active ingredients in TCMs.

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“…BHD was prepared according to previous studies (Liao et al 2018, Shen et al 2016. All ingredients were purchased from the Guangzhou Zhixin Chinese Herbal Medicine Co. Ltd. (Guangzhou, China).…”

Section: Drug Administrationmentioning

confidence: 99%

Buyang Huanwu Decoction Alleviates Cerebral Ischemia-Reperfusion Injury via Sirtuin 1/Autophagy Pathway

Dong

et al. 2020

Preprint

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BackgroundStroke accounts for a large proportion of deaths from disease around world. Buyang Huanwu Decoction (BHD) is used to protect against stroke and stroke-induced disability for many years in China. However, the mechanism of BHD to protect against stroke is still confused. MethodsThe middle cerebral artery occlusion and reperfusion (MCAO-R) model was used to investigate in this study. The animals were administrated with BHD (5, 10 and 20 g/kg) or rapamycin respectively. Infarct size and modified neurological severity score (mNSS) on day 5 were calculated. Cellular changes around ischemic penumbra were showed by HE staining and Nissl staining. Protein expressions of nestin, brain-derived neurotrophic factor (BDNF), doublecortin on the X-chromosome (DCX) and autophagy-related protein in the cerebral peri-ischemic area were detected. ResultsIn results, the post-treatment with BHD relieved brain infarct size and improved neurological deficits in MCAO-R rats. BHD protected against MCAO-R-induced neuronal necrosis, obviously enhanced autophagy (increased Beclin 1 and LC3Ⅱ, decreased P62) and increased the protein expressions of nestin, BDNF and DCX. Meanwhile, BHD promoted the expression of Sirtuin1（SIRT1）, an important regulator of autophagy. ConclusionsIn conclusion, our data suggested that post-treatment with BHD could protect rat brain from ischemia-reperfusion injury via SIRT1/autophagy pathway.

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Biospecific isolation and characterization of angiogenesis-promoting ingredients in Buyang Huanwu decoction using affinity chromatography on rat brain microvascular endothelial cells combined with solid-phase extraction, and HPLC-MS/MS

Cited by 33 publications

References 41 publications

<p>Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS</p>

<p>Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS</p>

Cell Chromatography-Based Screening of the Active Components in Buyang Huanwu Decoction Promoting Axonal Regeneration

Buyang Huanwu Decoction Alleviates Cerebral Ischemia-Reperfusion Injury via Sirtuin 1/Autophagy Pathway

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