Biospecific immobilization of mannan–penicillin G acylase neoglycoenzyme on Concanavalin A-bead cellulose

Immobilization of Penicillin G Acylase: The Key to Optimum Performance

“…A synthetic glycoconjugate of penicillin G acylase was prepared by reaction of the latter with a mannan-derived polyaldehyde, followed by reduction of the resulting Schiffs bases and the unreacted aldehyde groups. [62] The support was prepared by activating cellulose beads with chloro-1,3,5-triazine followed by covalent attachment of concanavalin A. This latter carrier, which was only very lightly (1% wt) loaded with concanavalin A, absorbed a small amount of the penicillin G acylase-mannan conjugate.…”

“…[62] Second layers of concanavalin A and penicillin G acylase-mannan conjugate were successively deposited on the first immobilisate (bioaffinity layering), but the gain in activity was small. [62] There was little activity loss upon storage or repeated reuse of the biocatalyst.…”

Magnetic Parkia pendula seed gum as matrix for Concanavalin A lectin immobilization and its application in affinity purification

“…For instance, the lectin extracted from Canavalia ensiformis, named Concanavalin A (Con A) has been extensively used in the isolation, fractioning and structural characterization of glycoproteins (Bucur et al 2004, Uygun et al 2012) and other important glycoconjugates bearing glucose and/or mannose residues (Fraguas et al 2004). Among the matrices used in protein immobilization, those which contain carbohydrates have become the focus of intense interest in biotechnology (Fraguas et al 2003, Mislovicová et al 2004, Angeli et al 2009). Furthermore, to increase their performance, matrices have been magnetized to decrease the processing time of samples, the utilization of chemicals as well as to facilitate separation and process automation (Pan et al 2005, Angeli et al 2009).…”

Development of immobilized systems with penicillin acylase from Esherichia coli for production of semisyntetic penicillins

“…Na primer, sintetički glikokonjugat penicilin-acilaze se može dobiti u reakciji sa polialdehidom dobijenim iz manana. Nakon toga se izvrši redukcija rezultujućih Šifovih baza i neproreagovalih aldehidnih grupa [127]. Pre imobilizacije enzima za nosač je neophodno hemijski vezati konkanavalin A. Iz tog razloga se priprema nosača vrši u dva stupnja.…”

“…Na osnovu literaturnih podataka, dobijen je nosač koji sadrži svega 1% konkanavalina A i koji je mogao da veže male količine sintetičkog glikoproteina PACmanan. Aktivnost imobilisanog enzima je bila 18 BPU/g, koja odgovara prinosu aktivnosti od 74% [127]. Dobijeni imobilizat je pokazao i veliku stabilnost tako da se javlja mali gubitak aktivnosti usled skladištenja ili ponovnog korišćenja biokatalizatora.…”