Biosorption Performance of Encapsulated Candida krusei for the removal of Copper(II)

Luk, Chi Him; Yip, Joanne; Yuen, C.W.M.; Pang, Siu-Kwong; Lam, Kim Hung; Kan, Chi Wai

doi:10.1038/s41598-017-02350-7

Cited by 24 publications

(20 citation statements)

References 37 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3C). 25 Besides the corresponding characteristic absorption peaks, the symmetric and asymmetric stretching of C-H at 2947 and 2891 cm À1 were observed. The characteristic peaks of 3,6-anhydro-L-galactose skeletal banding was observed at 931, 894 and 770 cm À1 in composite AG-CH scaffold, which conrms the presence of agarose.…”

Section: Ftir Spectroscopy and Thermal Property Of Scaffoldmentioning

confidence: 97%

See 1 more Smart Citation

Preparation of a sponge-like biocomposite agarose–chitosan scaffold with primary hepatocytes for establishing an in vitro 3D liver tissue model

Tripathi

Melo

2015

RSC Adv.

View full text Add to dashboard Cite

Designing a three-dimensional (3D) macroporous scaffold with desired bio-functional properties is an important aspect for fabricating an in vitro liver tissue model with applications in pre-clinical therapeutics testing. In the present study a bio-polymeric composite scaffold of agarose-chitosan (AG-CH) was synthesized at optimized sub-zero temperature and evaluated for its suitability in in vitro liver tissue engineering. The scaffold showed high porosity (83 AE 2%) with interconnected pores (average pore diameter 40-70 mm). High swelling kinetics on account of the hydrophilic pore channels in the AG-CH scaffold allows unhindered migration of cells and gaseous exchange. At neutral pH, the negative charge on the surface of the AG-CH scaffold ensures increased cell-to-cell interfacial interaction followed by colonization of hepatocytes. Rheological studies of the hydrated scaffold demonstrate its high spongelike visco-elastic behavior without any fracture deformation up to 34 AE 1 N, which insinuates its applicability for soft-tissue engineering. The AG-CH scaffold showed $15% degradation in a span of four weeks in sterile PBS at physiological pH, which could help to maintain the structural integrity of neotissue formation. In vitro primary hepatocytes proliferation in the AG-CH scaffold showed an increase in cellular metabolic activity. The hepatic functions like albumin secretion and urea synthesis were established for the primary hepatocytes in the 3D scaffold and were higher in comparison to the control.The expression of hepatic CYP450 biomarker was observed in the in vitro cultured hepatocytes immobilized in the 3D AG-CH scaffold. Thus, the AG-CH scaffold with suitable physico-chemical properties and hepatic cell compatibility present its potential for developing an in vitro liver tissue model.

show abstract

Section: Ftir Spectroscopy and Thermal Property Of Scaffoldmentioning

confidence: 97%

“…25 In brief, glutaraldehyde-crosslinked AG-CH scaffold was dried ($10 mg dried mass) at 60 C for 4 h. The scaffold was then ground using mortar-pestle and suspended in 5 mL of ultra pure H 2 O. Zeta potential analysis.…”

Section: Physico-chemical Characterization Of Scaffoldsmentioning

confidence: 99%

Preparation of a sponge-like biocomposite agarose–chitosan scaffold with primary hepatocytes for establishing an in vitro 3D liver tissue model

Tripathi

Melo

2015

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…These results are similar to those reported for Pichia jadinii M9 and Pichia anomala M10. More rapidly with an increment in temperature, with an optimum value of 30°C [33], in the removal of copper for encapsulated Candida krusei [34], and for C. neoformans, with a maximum adsorption capacity for Cr(VI) ion was observed at 28°C [18]. When the temperature increases, the rate of removal of Cr(VI) increases and the contact time required for complete removal of the metal decreases, increasing the redox reaction rate [35].…”

Section: Effect Of the Temperaturementioning

confidence: 99%

Biosorption of Heavy Metals by Candida albicans

Rodríguez¹,

González²,

Juárez³

et al. 2018

Advances in Bioremediation and Phytoremediation

View full text Add to dashboard Cite

The objective of this work was to study the resistance and removal capacity of heavy metals by the yeast Candida albicans. The resistance of some heavy metals was analyzed: the yeast grows in 2000 ppm of chromium, zinc, lead, and copper, 1500 ppm of arsenic (III), 500 ppm of silver, and little bit in cobalt (300 ppm) and mercury and cadmium (200 ppm). Analyzing its potential to remove heavy metals, it can efficiently remove is as follows: Cr(VI) (76%), lead (57%), silver (51%), cadmium (46%), fairly arsenic(III) (40% with the modified biomass), cobalt (37%), mercury (36%), copper (31%), little bit zinc (22%), and fluoride (10%). We determine the optimal characteristics for chromium(VI) removal in living cells and death biomass. The ideal conditions for the removal of 50 mg/L of Cr(VI) in living cells were 28°C, pH 7.0, and 10 × 10 6 yeast/mL, with glycerol-like carbon source. In dead yeast biomass, the ideal conditions for removal of metal are 200 mg/L of Cr(VI), 60°C, pH 1.0, 20 h, and 5 g of biomass.

show abstract

“…Yeasts which are used in the enzymatic industry and medicine can survive in a medium containing low or high concentrations of heavy metals (Cottet et al 2020). One of the most important microbial source for biosorption of heavy metals is Candida species (Luna et al 2016), which were shown to play an important role in the accumulation of metal ions (Honfi et al 2016, Luk et al 2017. Metal uptake capacity of Candida species under various experimental conditions depends on the metal type and the yeast species itself (Legorreta-Castañeda et al 2020).…”

Section: Introductionmentioning

confidence: 99%

PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS

Mersin

Açıkel

2021

Trakya University Journal of Natural Sciences

View full text Add to dashboard Cite

Yeasts can accumulate heavy metals and grow in acidic media. In the present study, it was shown that Candida yeasts in an aqueous solution accumulate single Cu(II) and Ni(II) cations. The effect of heavy metal ions on the specific growth rate of biomasses and the uptake of metal ions during the growth phase was investigated in a batch system. Bioaccumulation efficiency decreased with increasing metal ion concentrations at constant sucrose concentrations. Both the specific growth rate and the biomass concentration were more inhibited in the bioaccumulation media containing Ni(II) ions singly as compared with the bioaccumulation media containing Cu(II) ions singly. The maximum specific growth rate and the saturation constant of yeasts were examined with a double-reciprocal form of Monod equation. Metal uptake performance decreased from 81.68% to 46.28% with increasing Ni(II) concentration from 25 mg/L to 250 mg/L for Candida lipolytica. Candida biomasses may be an alternative way of removal of heavy metals from wastewaters and may constitute a sample to produce new biomass. The study showed that Candida yeasts can be used as economical biomass due to their metal resistance and efficient production.Özet: Mayalar, asidik ortamda büyüyebilir ve ağır metalleri biriktirebilir. Bu çalışma, Candida türü mayaların sulu çözeltilerden tekli Cu(II) ve Ni(II) katyonlarını biriktirdiğini göstermiştir. Ağır metal iyonlarının, biyokütlelerin spesifik büyüme hızı ve büyüme periyodu boyunca metal iyonlarını giderimi üzerindeki etkisi, bir kesikli sistemde araştırılmıştır. Sabit sakaroz derişiminde, metal iyonu derişimi arttıkça, biyobirikim verimi azalmıştır. Hem spesifik büyüme hızı hem de biyokütle konsantrasyonu, tek başına Cu(II) iyonları içeren biyoakümülasyon ortamına kıyasla Ni(II) iyonları içeren biyoakümülasyon ortamında daha fazla inhibe edilmiştir. Mayaların maksimum özgül büyüme hızı ve doygunluk sabiti, Monod denkleminin çift-karşılıklı formu ile incelenmiştir. Candida lipolytica'nın metal giderim performansı Ni(II) derişiminin 25 mg/L'den 250 mg/L'ye çıkmasıyla % 81,68'den % 46,28'e düşmüştür. Candida biyokütleleri, ağır metallerin atık sulardan gideriminde alternatif bir yol olabilir ve yeni biyokütle üretimi için bir örnek oluşturabilir. Bu çalışma, Candida mayalarının, metal direnci ve verimli üretimleri nedeniyle ekonomik biyokütle olarak kullanılabileceğini göstermektedir.

show abstract

Biosorption Performance of Encapsulated Candida krusei for the removal of Copper(II)

Cited by 24 publications

References 37 publications

Preparation of a sponge-like biocomposite agarose–chitosan scaffold with primary hepatocytes for establishing an in vitro 3D liver tissue model

Preparation of a sponge-like biocomposite agarose–chitosan scaffold with primary hepatocytes for establishing an in vitro 3D liver tissue model

Biosorption of Heavy Metals by Candida albicans

PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS

Contact Info

Product

Resources

About