Biosorption of some Heavy Metals by Deinococcus radiodurans Isolated from Soil in Basra Governorate-Iraq

Abstract

IntroductionBioremediation is a natural process which depends on bacteria, fungi, and plants to alternating pollutants as these organisms carry out their normal life functions. Thus, bioremediation offers a substitute tool to destroy or reduce the harmful pollutants through biological activity with an effective cost. In the early 1980s, certain microorganisms were found to accumulate metallic elements at a high capacity [1,2].There are a lot of studies about the bioremediation ability of D. radiodurans and its genetic engineering, for cleaning up heavy metals in nuclear waste contaminated sites [3][4][5][6][7]. The development of bioremediation strategies using Deinococcus spp is therefore vital for the cleanup of contaminated site with radioactive waste. Additional advantages of deinococci are that they are vegetative, easily cultured, and nonpathogenic. As these sites rarely contaminated by a single chemical, it is necessary to bio remediating strain to be multi resistant to various toxic agents. The present work describes the use of a combination of spectroscopic and microscopic methods to characterize the heavy metals around the cells of bacterial strains isolated from extreme habitats as well as to elucidate the interaction mechanisms of these bacteria with these metals. Materials and Methods Source of bacterial isolateThe tested isolates, D. radiodurans used in this study was isolated previously from the Um -Qasr district, south of Basra city-Iraq. The isolate was identified by biochemical tests and sequencing 16S rRNA gene and comparing the sequences online with GenBank database (www.ncbi.nlm.nih.gov). Biosorption experimentThe equilibrium, kinetics data of the biosorbent D. radiodurans were obtained by performing batch experiments AbstractThe bacterium Deinococcus radiodurans has been isolated from Basra soil. On the basis of morphological, biochemical, 16S rRNA gene sequencing and phylogeny analysis, the isolates were authentically identified as D. radiodurans. The biosorption capabilities of D. radiodurans for cadmium (Cd +2 ) and lead (Pb +2 ) were monitored at different concentrations and contact times. The characterization of heavy metals around the cells of bacterial strains was observed using the Fourier Transform Infrared (FT-IR) spectrophotometer, X-ray powder diffraction analysis (XRD) and transmission electron microscope (TEM).preparation UATR). This analysis was done in the Chemistry department, University Putra Malaysia.

Bioremediation of lead and cadmium and the strive role of Pediococcus pentosaceus probiotic

“…Fish are the water inhabitants, that can be heavily affected by heavy metals. In spite of the constitutive efforts to protect the health from hazardous heavy metals, occupational and environmental exposures to Pb and Cd remain a serious problem in many countries (2). Several modified and sophisticated techniques have emerged, including many methods of biological treatment to improve the environment contaminated with heavy metals.…”

Applications of Box–Behnken experimental design coupled with artificial neural networks for biosorption of low concentrations of cadmium using Spirulina (Arthrospira) spp.

“…Cadmium is one of the most toxic heavy metals found in drinking water. It is reported in most of the recent studies on heavy metal removal at low concentrations, the biological adsorption is more economical and superior when compared with other adsorption process [1][2][3][4].…”