Biosorption and degradation of decabromodiphenyl ether by Brevibacillus brevis and the influence of decabromodiphenyl ether on cellular metabolic responses

Abstract

There is global concern about the effects of decabromodiphenyl ether (BDE209) on environmental and public health. The molecular properties, biosorption, degradation, accumulation, and cellular metabolic effects of BDE209 were investigated in this study to identify the mechanisms involved in the aerobic biodegradation of BDE209. BDE209 is initially absorbed by wall teichoic acid and N-acetylglucosamine side chains in peptidoglycan, and then, BDE209 is transported and debrominated through three pathways, giving tri-, hepta-, octa-, and nona-bromodiphenyl ethers. The C-C bond energies decrease as the number of bromine atoms on the diphenyl decreases. Polybrominated diphenyl ethers (PBDEs) inhibit protein expression or accelerate protein degradation and increase membrane permeability and the release of Cl(-), Na(+), NH4 (+), arabinose, proteins, acetic acid, and oxalic acid. However, PBDEs increase the amounts of K(+), Mg(2+), PO4 (3-), SO4 (2-), and NO3 (-) assimilated. The biosorption, degradation, accumulation, and removal efficiencies when Brevibacillus brevis (1 g L(-1)) was exposed to BDE209 (0.5 mg L(-1)) for 7 days were 7.4, 69.5, 16.3, and 94.6 %, respectively.

Bacterial Biodegradation of 4-Monohalogenated Diphenyl Ethers in One-Substrate and Co-Metabolic Systems

“…Considering also the specific plasmid-encoded hydrocarbon degradation pathway described for the P. plecoglossicida IsA strain by Marqués and Ramos [44], the addition of phenol might counterweight the negative effect of diphenyl ether on the membrane, thus slightly reducing the lipid bilayer permeability. In contrast, we observed an increase in P. fluorescens B01 membrane permeability, which might result from oxidative stress, as reported in [45]. Also, phenol addition might elicit efflux of potassium ions from the membrane, as in the case of E. coli [46], thus accelerating membrane permeability.…”

Cell changes and differential proteomic analysis during biodegradation of decabromodiphenyl ether (BDE-209) byPseudomonas aeruginosa

“…Cell membrane permeability of P. aeruginosa was evaluated by measuring the release of β-galactosidase activity into the culture medium using o -nitrophenyl-β- d -galactopyranoside (ONPG) as a substrate. 13 Pre-cultivated bacteria was pipetted into Erlenmeyer flasks containing 100 mL MSM and 1% lactose, and incubated on a rotary shaker at 35 °C for 10 h. Then the solutions were centrifuged at 8000 g for 5 min, washed three times and re-suspended in β-galactosidase buffer. To the buffer was added BDE-209 at 20 mg L −1 then mixed with ONPG at 1 mg mL −1 .…”

Aerobic Degradation Characteristics of Decabromodiphenyl ether through Rhodococcus ruber TAW-CT127 and Its Preliminary Genome Analysis

“…In response to external stimuli, the bacteria and pollutants interact and BDE-209 is translocated intracellularly and degraded, a process that leads to a series of changes in cellular characteristics [ 37 ]. PBDEs inhibit the expression of bacteriophage proteins, thereby increasing the permeability of the cell membrane [ 51 ]. The strains were inoculated in different nutritional state media for 5 days, followed by SEM observation, which shows that TAW-CT127 is micro-rod-shaped under eutrophic conditions, and that the surface is smooth and round ( Figure 4 a).…”