Biosíntesis inducida de fengicina y surfactina en una cepa de Bacillus amyloliquefaciens con actividad oomiceticida sobre zoosporas de Phytophthora capsica

Ley-López, Nancy; Heredia, José Basilio; Martín-Hernández, César San; Ibarra-Rodríguez, J. R.; Angulo-Escalante, Miguel Ángel; García‐Estrada, Raymundo Saúl

doi:10.1016/j.ram.2022.03.002

Cited by 4 publications

(9 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…on P. capsici included inhibition of mycelial growth, , suppression of sporangium formation and zoosporogenesis, , inhibition of germination on zoospores, − and lysis of zoospores. , The active secondary metabolites from these biocontrol strains directly contributing to the biocontrol of P. capsici are 1 H -pyrrole-2-carboxylic acid, a volatile compound (2,4-di- tert -butylphenol) and CLPs putisolvin, surfactin, and fengycin .…”

Section: Discussionmentioning

confidence: 99%

“…16,44 The active secondary metabolites from these biocontrol strains directly contributing to the biocontrol of P. capsici are 1H-pyrrole-2-carboxylic acid, 41 a volatile compound (2,4-di-tert-butylphenol) 42 and CLPs putisolvin, 16 surfactin, and fengycin. 44 Moreover, we have shown in this study that CLPs pseudophomins A−D (1− 4) are capable of decreasing the incidence of disease symptoms caused by P. capsici on cucumber leaf discs via the lysis of zoospores of this pathogen (Figure 6). Additionally, the herbicidal (such as Setaria viridis) and antifungal (such as Rhizoctonia solani, Sclerotinia sclerotiorum, Alternaria brassicae, and Phoma lingam) activities of pseudophomins A and B have been reported previously.…”

Section: ■ Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Pseudophomins A–D Produced from Pseudomonas sp. HN8-3 Using an OSMAC Approach and Their Roles in Biocontrol of Phytophthora capsici in Cucumbers

Sheng

2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

In this study, two new cyclic lipopeptides (CLPs) pseudophomins C (3) and D (4) and two known CLPs pseudophomins A (1) and B (2) were produced and characterized from the bacterial supernatant of Pseudomonas sp. HN8-3 by an OSMAC (one strain-many compounds) approach. OSMAC is a strategy that involves feeding of a single microorganism with divergent substrates to stimulate the production of new secondary metabolites. These pseudophomins were purified and identified via chromatographic methods, droplet collapse assay, genome mining, spectroscopic and spectrometric analyses, and single-crystal X-ray diffraction (XRD). Moreover, bioactivity tests showed that pseudophomins could lyse the zoospores of Phytophthora capsici in vitro, and coapplication of pseudophomins with zoospores of P. capsici further reduced the incidence of P. capsici on cucumber leaves. Collectively, these results indicated that pseudophomins have the potential to be developed as biopesticides for controlling P. capsici in cucumber.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Pseudophomins A–D Produced from Pseudomonas sp. HN8-3 Using an OSMAC Approach and Their Roles in Biocontrol of Phytophthora capsici in Cucumbers

Sheng

2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…4,6 Although CLPs surfactin (originated from Bacillus spp. ), 7 fengycin (originated from Bacillus spp. ), 7 putisolvin (originated from Pseudomonas spp.)…”

Section: ■ Introductionmentioning

confidence: 99%

“…), fengycin (originated from Bacillus spp. ), putisolvin (originated from Pseudomonas spp. ) and pheudophomin (originated from Pseudomonas spp.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

WLIP, WLIPβ, and WLIPγ Produced from Pseudomonas canadensis Q3–1 via Precursor-Directed Biosynthesis and Their Roles on Biocontrol of Phytophthora Blight in Peppers

Ma,

Yang,

Sheng

2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

White line-inducing principle (WLIP, 1), together with two new cyclic lipopeptides (CLPs) WLIPβ (2) and WLIPγ (3), were characterized from the supernatant of Pseudomonas canadensis Q3−1 via precursor-directed biosynthesis (PDB) in the current study. They were purified from the supernatant of P. canadensis Q3−1 by solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC), and their structures were mainly determined via bioinformatic analyses, spectrometric and spectroscopic techniques, as well as single crystal X-ray diffraction (XRD). These WLIPs share (R)-3-hydroxydecanoic acid (HDA), but they differ from each other in the composition of peptidic sequences. In addition, these CLPs showed biocontrol activities against Phytophthora blight (caused by Phytophthora capsici) in peppers. Collectively, this study has shown that PDB could be used for generating new CLPs in Pseudomonas spp. Moreover, we have confirmed that WLIP, WLIPβ, and WLIPγ could be used as lead agrochemicals to control Phytophthora blight in peppers.

show abstract

Identification and Quantification of Lipopeptide Homologues Induced and Produced by Bacillus amyloliquefaciens

Ley-López,

Heredia,

Martín-Hernández

et al. 2023

Fermentation

View full text Add to dashboard Cite

Cyclic lipopeptides (LPs) are potentially promising in the agricultural, industrial and pharmaceutical sectors. LPs have a variable hydrophilic cyclic peptide part attached to a variable fatty acid chain. One limitation of these compounds is their low availability due to their limited production by bacteria. The objective of this study was to identify and quantify homologues of LPs biosynthesized by Bacillus amyloliquefaciens using ultra-performance liquid chromatography (UPLC–MS/MS) after inducing the synthesis of these secondary metabolites using different inducers, including chemical compounds and inactive cells of Colletotrichum sp. Four homologues were identified in the iturin family (bacillomycin D), and the iturin homologue with the highest synthesis was the molecular ion m/z 1031.54, with 173.1 µg mg−1 crude extract. In addition, seven homologues were detected in the fengycin family (four of fengycin A and three of fengycin B), and the homologue with the highest content was the molecular ion m/z 1463.69 (fengycin A), with 3288 ± 528.5 ng mg−1 crude extract. Finally, five homologues were identified in the surfactin family, where the highest concentration was observed for the molecular ion m/z 1036.68, with 61.5 ± 3.01 µg mg−1 crude extract. The highest concentration of LP homologues (iturin, fengycin and surfactin) synthesized by B. amyloliquefaciens was detected in the presence of inactive cells of Coletotrichum sp., suggesting that the inducing substance is associated with the inducer’s cell envelope and could be a single protein or a structure that includes protein components.

show abstract

Biosíntesis inducida de fengicina y surfactina en una cepa de Bacillus amyloliquefaciens con actividad oomiceticida sobre zoosporas de Phytophthora capsica

Cited by 4 publications

References 47 publications

Pseudophomins A–D Produced from Pseudomonas sp. HN8-3 Using an OSMAC Approach and Their Roles in Biocontrol of Phytophthora capsici in Cucumbers

Pseudophomins A–D Produced from Pseudomonas sp. HN8-3 Using an OSMAC Approach and Their Roles in Biocontrol of Phytophthora capsici in Cucumbers

WLIP, WLIPβ, and WLIPγ Produced from Pseudomonas canadensis Q3–1 via Precursor-Directed Biosynthesis and Their Roles on Biocontrol of Phytophthora Blight in Peppers

Identification and Quantification of Lipopeptide Homologues Induced and Produced by Bacillus amyloliquefaciens

Contact Info

Product

Resources

About