Bioseparation of papain from Carica papaya latex by precipitation of papain–poly (vinyl sulfonate) complexes

Braia, Mauricio; Ferrero, Maximiliano Ruben; Rocha, María Victoria; Loureiro, Dana Belén; Tubío, Gisela; Romanini, Diana

doi:10.1016/j.pep.2013.07.008

Cited by 11 publications

(4 citation statements)

References 22 publications

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“…pVS has been explored before as an antithrombogenic agent, , HIV-inhibitor, coatings for antibacterial surfaces, and as polyelectrolytes for antibody and protein purification processes, − but never before as a coactivator for FGFRs. From screening studies, we identified pVS from other polysulfonated polymers as a new heparin-mimicking polymer.…”

Section: Resultsmentioning

confidence: 99%

Poly(vinyl sulfonate) Facilitates bFGF-Induced Cell Proliferation

et al. 2015

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Heparin is a highly sulfated polysaccharide and useful because of its diverse biological functions. However, because of batch-to-batch variability and other factors, there is significant interest in preparing biomimetics of heparin. To identify polymeric heparin mimetics, a cell-based screening assay was developed in cells that express fibroblast growth factor receptors (FGFRs) but not heparan sulfate proteoglycans. Various sulfated and sulfonated polymers were screened and poly(vinyl sulfonate) (pVS) was identified as the strongest heparin-mimicking polymer in its ability to enhance binding of basic fibroblast growth factor (bFGF) to FGFR. The results were confirmed by an ELISA-based receptor-binding assay. Different molecular weights of pVS polymer were synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization. The polymers were able to facilitate dimerization of FGFRs leading to cell proliferation in FGFR-expressing cells, and no size dependence was observed. The data showed that pVS is comparable to heparin in these assays. In addition, pVS was not cytotoxic to fibroblast cells up to at least 1 mg/mL. Together this data indicates that pVS should be explored further as a replacement for heparin.

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Section: Resultsmentioning

confidence: 99%

Poly(vinyl sulfonate) Facilitates bFGF-Induced Cell Proliferation

et al. 2015

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“…The nonmonotonic trend with papain and the t-6-Dex:SB ratio was suggestive of an unfavorable interaction with SB ligands that was mitigated by the increasing proportion of t-6-Dex ligand. As discussed further in the Supporting Information, the root cause is unclear but may be related to binding interactions between sulfonates and papain. − …”

Section: Resultsmentioning

confidence: 99%

Assessing the Steric Impact of Surface Ligands on the Proteolytic Turnover of Quantum Dot–Peptide Conjugates

Krause,

Rees,

Algar

2023

ACS Appl. Mater. Interfaces

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“…Precipitants that do not denature biological products, such as enzymes, may also be used, forming a precipitate that is normally more stable than the soluble material (Cortez and Pessoa Jr., 1999;Golunski et al, 2011;Soares et al, 2012). Most common precipitants involve salts, polyelectrolytes and organic solvents (Golunski et al, 2011;Soares et al, 2012;Valetti et al, 2012;Braia et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Influence of Different Solvents in the Recovery and Pre-Purification of Pectinases from <em>Aspergillus niger</em>

Trentini¹,

Rossetto²,

Fernandes³

et al. 2018

AJFST

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The present work aimed at studying the influence of different solvents (n-propyl alcohol, isopropyl alcohol and acetone) in the recovery and pre-purification of pectinases (exo-polygalacturonase-exo-PG, pectinmethylesterase-PME and pectin lyase-PMGL) from Aspergillus niger. The best results were obtained using isopropyl alcohol in the precipitated phase with purification factor and recovery of 2.1-fold and 20.5% for exo-PG, 7.9-fold and 77.8% for PME and 2.5-fold and 216% for PMGL enzyme. The studied method is promising in the recovery and pre-purification of pectinases enzymes.

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Bioseparation of papain from Carica papaya latex by precipitation of papain–poly (vinyl sulfonate) complexes

Cited by 11 publications

References 22 publications

Poly(vinyl sulfonate) Facilitates bFGF-Induced Cell Proliferation

Poly(vinyl sulfonate) Facilitates bFGF-Induced Cell Proliferation

Assessing the Steric Impact of Surface Ligands on the Proteolytic Turnover of Quantum Dot–Peptide Conjugates

Influence of Different Solvents in the Recovery and Pre-Purification of Pectinases from <em>Aspergillus niger</em>

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