Biosensors for the Detection of OP Nerve Agents

Anzai, Jun Ichi

doi:10.1016/b978-0-12-800159-2.00062-2

Cited by 8 publications

(3 citation statements)

References 26 publications

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“…The products of these reactions are then oxidized or reduced by electrochemistry, creating an electric current as the output signal. Therefore, selectively the chemical signal (i.e., the type of analyte and its concentration) is transformed into an electrical signal through the enzyme/electrode interface (Anzai, 2015;Soropogui et al, 2018).…”

Section: Optimization Of the Biosensormentioning

confidence: 99%

A Novel Enzyme Biosensor Based on Ag/Reduced Graphene Oxide/Chitosan Membrane with Potentiometer for Pesticide Detection

Mashuni

Yanti

Irnawati

et al. 2023

molekul

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Long-term accumulation of pesticides in the environment to human and animal health. Acetylcholinesterase (AChE) biosensors with highly sensitive potentiometer transducers based on the membranes of Ag, reduced graphene oxide (rGO), and chitosan (CS) has been successfully developed. The membrane was made with a composition of 0.5 mM AgNO3, 2.5 mg/mL rGO, and 2% (w/v) CS coated on the surface of the Au electrode. The composition of the membrane with three ratios, namely 1:1:2, 2:1:3, and 3:1:4. Then, membrane Ag/rGO/CS and the enzyme AChE were immobilized on the membrane surface. The prepared biosensor has excellent conductivity, catalytic activity, and biocompatibility attributed to the synergistic effect of Ag/rGO/CS and glutaraldehyde (GTA) as crosslinkers and providing a hydrophilic surface for AChE adhesion. The linear range in biosensors is 1 × 10-8 to 1 µg L-1 with a regression coefficient of 0.9803 for 1:1:2 membrane, 0.9836 for 2:1:3 membrane, and 0.9850 for 3:1:4 membrane. The LOD is about 1 × 10-7 µg L-1 for all membranes. In addition, the biosensor showed good sensitivity, acceptable reproducibility, and stability, having an RSD of less than 5%. This biosensor makes it possible to provide a new and promising tool for analyzing pesticides, especially organophosphates.

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Section: Optimization Of the Biosensormentioning

confidence: 99%

A Novel Enzyme Biosensor Based on Ag/Reduced Graphene Oxide/Chitosan Membrane with Potentiometer for Pesticide Detection

Mashuni

Yanti

Irnawati

et al. 2023

molekul

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“…The notion of amperometric measurements with the use of AChE biosensors are usually associated with the decomposition of the acetylthiocholine (leading to electroactive product) into thiocholine and acetic acid. Subsequently, the resulting thiocholine could be electrochemically oxidized to its dimer (dithiobischoline) on the electrode surface [11].…”

Section: Introductionmentioning

confidence: 99%

An Electrochemical Acetylcholinesterase Biosensor Based on Fluorene(bisthiophene) Comprising Polymer for Paraoxon Detection

Celik

Söylemez

2022

Electroanalysis

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In this study, a novel conductive polymer comprising biosensor based on poly-2,2'-(9,9-dioctyl-9 hfluorene-2,7-diyl)bistiophene (Poly(BT)) and acetylcholinesterase (AChE) was reported for the determination of paraoxon. This practical biosensor allowed to catalyze electrochemical oxidation of acetylthiocholine (+ 0.6 V vs. Ag reference). The detection range for acetylcholine chloride (AThCl) with Poly(BT)/AChE was found to be 0.025-4 mM. In pesticide analysis, wide linear ranges from 0.5 to 1 μg/L and 1 to 14 μg/L, and a low detection limit of 0.033 μg/L were estimated. Under optimum operating conditions, the developed biosensor was used for pesticide detection in milk and tap water samples, effectively.

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“…Thus, a strong need exists to develop concise methods for sensing phosgene gas. Fluorescence and color-based chemical sensors are of current interest owing to several advantageous characteristics such as high sensitivity and rapid rates of response. − However, in contrast to the wide number of fluorescence and color sensors for nerve gas agents that have been reported, − only a few efforts have focused on developing sensors for phosgene. − …”

mentioning

confidence: 99%

Colorimetric and Fluorescent Detecting Phosgene by a Second-Generation Chemosensor

Zhou

Jung

et al. 2018

Anal. Chem.

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Because of the current shortage of first-generation phosgene sensors, increased attention has been given to the development of fluorescent and colorimetric based methods for detecting this toxic substance. In an effort focusing on this issue, we designed the new, second-generation phosgene chemosensor 1 and demonstrated that it undergoes a ring-opening reaction with phosgene in association with color and fluorescent changes with a detection limit of 3.2 ppb. Notably, in comparison with the first-generation sensor RB-OPD, 1 not only undergoes a much faster response toward phosgene with an overall response time within 2 min, but it also generates no byproducts during the sensing process. Finally, sensor 1 embedded nanofibers were successfully fabricated and used for accurate and sensitive detection of phosgene.

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Biosensors for the Detection of OP Nerve Agents

Cited by 8 publications

References 26 publications

A Novel Enzyme Biosensor Based on Ag/Reduced Graphene Oxide/Chitosan Membrane with Potentiometer for Pesticide Detection

A Novel Enzyme Biosensor Based on Ag/Reduced Graphene Oxide/Chitosan Membrane with Potentiometer for Pesticide Detection

An Electrochemical Acetylcholinesterase Biosensor Based on Fluorene(bisthiophene) Comprising Polymer for Paraoxon Detection

Colorimetric and Fluorescent Detecting Phosgene by a Second-Generation Chemosensor

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