Biosensors for pesticide detection based on alkaline phosphatase-catalyzed chemiluminescence

Ayyagari, Madhu S.; Kamtekar, Sanjay; Pande, Rajiv; Marx, Kenneth A.; Kumar, Jayant; Tripathy, Sukant K.; Kaplan, David L.

doi:10.1016/0928-4931(95)00077-1

Cited by 25 publications

(6 citation statements)

References 8 publications

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“…AJAC been developed using different enzyme substrates depending on the transduction method. Ayyagari et al described a chemiluminescent ALPbased biosensor for the detection of paraoxon [30]. The biosensor was based on the measurement of the intensity of the light generated by ALP-catalyzed dephosphorylation of a chemiluminescent substrate, chloro 3-(4-methoxy spiro [1,2-…”

Section: Alkaline Phosphatase (Alp)-based Biosensorsmentioning

confidence: 99%

Biosensors for Pesticide Detection: New Trends

Sassolas¹,

Prieto‐Simón²,

Marty³

2012

AJAC

186

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Due to the large amounts of pesticides commonly used and their impact on health, prompt and accurate pesticide analysis is important. This review gives an overview of recent advances and new trends in biosensors for pesticide detection. Optical, electrochemical and piezoelectric biosensors have been reported based on the detection method. In this review biosensors have been classified according to the immobilized biorecognition element: enzymes, cells, antibodies and, more rarely, DNA. The use of tailor-designed biomolecules, such as aptamers and molecularly imprinted polymers, is reviewed. Artificial Neural Networks, that allow the analysis of pesticide mixtures are also presented. Recent advances in the field of nanomaterials merit special mention. The incorporation of nanomaterials provides highly sensitive sensing devices allowing the efficient detection of pesticides

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Section: Alkaline Phosphatase (Alp)-based Biosensorsmentioning

confidence: 99%

Biosensors for Pesticide Detection: New Trends

Sassolas¹,

Prieto‐Simón²,

Marty³

2012

AJAC

186

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“…Chemiluminescence is another method that has been used for the detection of the enzymatic inhibition by pesticides [80]. In this case, alkaline phosphatase (ALP) was immobilised together with a polymer on a glass surface connected to a photomultiplier.…”

Section: Flow-based Enzymatic Sensing Systemsmentioning

confidence: 99%

Trends in Flow-based Biosensing Systems for Pesticide Assessment

Prieto‐Simón

Campàs

Andreescu

et al. 2006

Sensors

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This review gives a survey on the state of the art of pesticide detection using flow-based biosensing systems for sample screening. Although immunosensor systems have been proposed as powerful pesticide monitoring tools, this review is mainly focused on enzyme-based biosensors, as they are the most commonly employed when using a flow system. Among the different detection methods able to be integrated into flow-injection analysis (FIA) systems, the electrochemical ones will be treated in more detail, due to their high sensitivity, simple sample pretreatment, easy operational procedures and real-time detection. During the last decade, new trends have been emerging in order to increase the enzyme stability, the sensitivity and selectivity of the measurements, and to lower the detection limits. These approaches are based on (i) the design of novel matrices for enzyme immobilisation, (ii) new manifold configurations of the FIA system, sometimes including miniaturisation or lab-on-chip protocols thanks to micromachining technology, (iii) the use of cholinesterase enzymes either from various commercial sources or genetically modified with the aim of being more sensitive, (iv) the incorporation of other highly specific enzymes, such as organophosphate hydrolase (OPH) or parathion hydrolase (PH) and (v) the combination of different electrochemical methods of detection. This article discusses these novel strategies and their advantages and limitations.

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“…[15] This is the case for APA and EA which are also inhibited by other contaminants [15 -17] and already been used to produce biosensors which could be potentiometric, [15,16] amperometric, [17] or optical. [18,19] Therefore, biosensors using those enzymes are quite adapted for assessment of overall toxicity of waste water.…”

Section: Toxicity Assessment Of a Contaminated Effluentmentioning

confidence: 99%

A Bi‐enzymatic Whole‐Cell Algal Biosensor for Monitoring Waste Water Pollutants

et al. 2004

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Two algal whole cells biosensors are developed to measure specific toxicity of freshwater pollutants. Both optical and conductometric biosensors are based on inhibition of algal alkaline phosphatase (AP) and esterase activities. Chlorella vulgaris cells are immobilised on a membrane placed in front of an optical fiber bundle for optical sensing or deposited on the surface of an electrode for conductometric sensing. Phosphatase activity of the biosensor is strongly inhibited by heavy metal ions

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Biosensors for pesticide detection based on alkaline phosphatase-catalyzed chemiluminescence

Cited by 25 publications

References 8 publications

Biosensors for Pesticide Detection: New Trends

Biosensors for Pesticide Detection: New Trends

Trends in Flow-based Biosensing Systems for Pesticide Assessment

A Bi‐enzymatic Whole‐Cell Algal Biosensor for Monitoring Waste Water Pollutants

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