Biosensors for environmental control

Karube, Isao; Tamiya, Eiichi

doi:10.1351/pac198759040545

Cited by 29 publications

(9 citation statements)

References 5 publications

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“…Approaches to the use of biocatalysts other than sulfite oxidase were previously published. Sulfite ion sensor with use of immobilized organelles [24,25] and a microbial sensor of immobilized Thiobacillus thiooxidans cells [26] were described.…”

Section: Introductionmentioning

confidence: 99%

Composite Biosensor for Sulfite Assay: Use of Water-Insoluble Hexacyanoferrate(III) Salts as Electron-Transfer Mediators

et al. 1998

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Water-insoluble salts of hexacyanoferrate(III) and cationic surface active agents were synthetized and used as electron-mediators for sulfite oxidase. The biosensor was prepared from a composite consisting of modified graphite (50 % w/w) and n-eicosane (50 % w/w). Graphite was modified with mediators or with both mediator and sulfite oxidase for surface-and bulk-modified electrode, respectively. The main advantage of biosensors with insoluble mediators is the possibility to operate at a potential of 0 mV (vs. SCE), thus less interferences are expected, in comparison to soluble hexacyanoferrate(III) where a potential of +300 mV must be used. The maximum sensitivity 7.8 × 10 ¹4 mA/mM was obtained for bulkmodified biosensor, prepared from graphite modified with 5 % w/w of hexadecyltrimethylammonium hexacyanoferrate(III) and 1.25 units/mg (of graphite) of sulfite oxidase. The sensitivity of the biosensor decreased to 24 % of the initial sensitivity after one month storage in dry state at ambient temperature. The use of trehalose as an enzyme stabilization agent has led to the improved stability: 40 % of the initial stability was retained after one month.

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Section: Introductionmentioning

confidence: 99%

Composite Biosensor for Sulfite Assay: Use of Water-Insoluble Hexacyanoferrate(III) Salts as Electron-Transfer Mediators

et al. 1998

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“…The cells become more susceptible to DNA damage and even with the slightest damage cell growth can be inhibited. In this test mutagenic compounds result in DNA degradation and cell growth inhibition, yet they do not cause mutation (Karube andTamiya 1987, Ko³wzan 2009). …”

Section: Fmnh 2 + Rcho + O 2 ® Fmn + Rcooh + H 2 O + Hí (490 Nm)mentioning

confidence: 99%

Biological methods used to assess surface water quality

Szczerbiñska

Gałczyńska

2015

Archives of Polish Fisheries

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In accordance with the guidelines of the Water Framework Directive 2000/60 (WFD), both ecological and chemical statuses determine the assessment of surface waters. The profile of ecological status is based on the analysis of various biological components, and physicochemical and hydromorphological indicators complement this assessment. The aim of this article is to present the biological methods used in the assessment of water status with a special focus on bioassay, as well as to provide a review of methods of monitoring water status. Biological test methods include both biomonitoring and bioanalytics. Water biomonitoring is used to assess and forecast the status of water. These studies aim to collect data on water pollution and forecast its impact. Biomonitoring uses organisms which are characterized by particular vulnerability to contaminants. Bioindicator organisms are algae, fungi, bacteria, larval invertebrates, cyanobacteria, macroinvertebrates, and fish. Bioanalytics is based on the receptors of contaminants that can be biologically active substances. In bioanalytics, biosensors such as viruses, bacteria, antibodies, enzymes, and biotests are used to assess degrees of pollution.

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“…Biosensors are classified according to the bioreceptor used or biological process involved such as biocatalytic (enzyme-based), immunologic (antibody-antigen based) or DNA (nucleic acid-based) sensors (25,(27)(28)(29)(30)(31)(32)(33). They can also be classified according to the type of signal transduction such as optical, electrical, thermal or mechanical (21,(34)(35)(36)(37). Biosensors are often developed for detecting target molecules in clinical samples but a number of biosensors also exist for ensuring food quality and safety; for monitoring and control of environmental contaminants and for addressing agricultural problems (35)(36)(37)(38)(39).…”

Section: Biosensorsmentioning

confidence: 99%

“…They can also be classified according to the type of signal transduction such as optical, electrical, thermal or mechanical (21,(34)(35)(36)(37). Biosensors are often developed for detecting target molecules in clinical samples but a number of biosensors also exist for ensuring food quality and safety; for monitoring and control of environmental contaminants and for addressing agricultural problems (35)(36)(37)(38)(39).…”

Section: Biosensorsmentioning

confidence: 99%

Bioconjugated nanomaterials on devices for infectious disease diagnostics

Ponce¹

2012

Front Biosci

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Biosensors for environmental control

Cited by 29 publications

References 5 publications

Composite Biosensor for Sulfite Assay: Use of Water-Insoluble Hexacyanoferrate(III) Salts as Electron-Transfer Mediators

Composite Biosensor for Sulfite Assay: Use of Water-Insoluble Hexacyanoferrate(III) Salts as Electron-Transfer Mediators

Biological methods used to assess surface water quality

Bioconjugated nanomaterials on devices for infectious disease diagnostics

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