Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds

Pohanka, Miroslav

doi:10.2478/s11696-014-0542-x

Cited by 27 publications

(18 citation statements)

References 126 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, it does not mean that BChE is completely useless. It is able to degrade some toxic compounds of natural origin as well as drugs such as cocaine and succinylcholine (Duysen & Lockridge, 2011;Lejus et al, 2006;Pohanka, 2015).…”

Section: Introductionmentioning

confidence: 99%

Determination of acetylcholinesterase and butyrylcholinesterase activity without dilution of biological samples

Pohanka

2015

Chemical Papers

Self Cite

View full text Add to dashboard Cite

Two cholinesterases: acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), are known. The enzymes are important in the body and alteration of their activity has significant use in the diagnosis of poisoning, liver function, etc. Currently available methods for the determination of cholinesterases have some major drawbacks including various interferences and the inability to be used for decreasing the enzyme activity in the presence of reversible inhibitors due to sample dilution; hence, a method for dilution free assay of cholinesterases is desired. Here, microplates were modified with indoxylacetate (100 µL of 10 mmol L −1 solution) and used for cholinesterases assay after drying at 37 • C. The fact that indoxylacetate remains stable in dry state and serves simultaneously as a chromogen and substrate provide good prerequisites for the method. The limit of detection for BChE was 0.71 U while that for AChE was 2.8 U per a 100 µL sample (solution of enzyme or plasma sample). The limit of detection is low enough to allow standard examination of cholinesterasemia. The two cholinesterases can be distinguished from each other using selective inhibitors such as donepezil and iso-OMPA. The new method was also successfully validated for the standard Ellman's assay using plasma samples with BChE activity adjusted by carbofuran. The new method based on indoxylacetate seems promising for routine tests.

show abstract

Section: Introductionmentioning

confidence: 99%

Determination of acetylcholinesterase and butyrylcholinesterase activity without dilution of biological samples

Pohanka

2015

Chemical Papers

Self Cite

View full text Add to dashboard Cite

show abstract

“…We investigated effect of boldine on both cholinesterases and found out that it is able to inhibit them. Dixon plot revealed noncompetitive mechanism of inhibition which is the most common inhibition mechanism for cholinesterases [ 13 ]. The inhibition of AChE by boldine was already investigated and authors reported IC 50 8.5 μ mol/l [ 8 ].…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Acetylcholinesterase and Butyrylcholinesterase by a Plant Secondary Metabolite Boldine

Kostelník

Pohanka

2018

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are two enzymes sensitive to various chemical compounds having ability to bind to crucial parts of these enzymes. Boldine is a natural alkaloid and it was mentioned in some older works that it can inhibit some kinds of AChE. We reinvestigated this effect on AChE and also on BChE using acetyl (butyryl) thiocholine and Ellman's reagents as standard substances for spectrophotometric assay. We found out IC50 of AChE equal to 372 μmol/l and a similar level to BChE, 321 μmol/l. We conclude our experiment by a finding that boldine is cholinesterase inhibitor; however we report significantly weaker inhibition than that suggested in literature. Likewise, we tried to investigate the mechanism of inhibition and completed it with in silico study. Potential toxic effect on cholinesterases in real conditions is also discussed.

show abstract

“…Glucose biosensors have been widely researched and used even though it was the first and the most commercialized type (Turner 2013;Pohanka 2015).…”

Section: Current Construction Of Glucose Biosensorsmentioning

confidence: 99%

Biosensors for Blood Glucose and Diabetes Diagnosis: Evolution, Construction, and Current Status

Martinková¹,

Pohanka²

2015

Analytical Letters

Self Cite

View full text Add to dashboard Cite

Diabetes mellitus is a worldwide health problem and complications associated with the disease are a significant cause of death in the world. Monitoring the blood glucose level is the first step during diagnosis of diabetes mellitus; hence rapid and accurate method of diagnosis is necessary for prevention of lethal complications. Current research is directed towards miniaturization of analytical equipment and also towards a decrease in consumption of biological materials and chemical substances. As a result, biosensors are available for sensitive, accurate, and low cost measurements. The first glucose enzymatic biosensor was proposed by Clark and Lyons (1962).Many different applications and innovations of biosensor have been performed since this work. Biosensors consist of a biorecognition element and physicochemical transducer providing aDownloaded by [New York University] at 01:28 22 June 20152 measurable signal. In case of glucose biosensors, enzyme glucose oxidase is used as a biorecognition element (or biotransducer in some sources) converting glucose to gluconic acid.An electrochemical device was the most common physicochemical transducer for many years, but the optical transducers are becoming more common now. In spite of the advantages of enzymatic glucose biosensors, many disadvantages also were encountered. Consequently, some researchers developed non-enzymatic glucose biosensors. This review covers evolution of glucose biosensors, construction of traditional and marketed types of biosensors, and promising applications.

show abstract

Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds

Cited by 27 publications

References 126 publications

Determination of acetylcholinesterase and butyrylcholinesterase activity without dilution of biological samples

Determination of acetylcholinesterase and butyrylcholinesterase activity without dilution of biological samples

Inhibition of Acetylcholinesterase and Butyrylcholinesterase by a Plant Secondary Metabolite Boldine

Biosensors for Blood Glucose and Diabetes Diagnosis: Evolution, Construction, and Current Status

Contact Info

Product

Resources

About