Biosensors coated with sulfated polysaccharides for the detection of hepatocyte growth factor/scatter factor in cell culture medium

Berger, Manuel; Welle, Alexander; Gottwald, Eric; Rapp, M.; Länge, Kerstin

doi:10.1016/j.bios.2010.07.065

Cited by 14 publications

(14 citation statements)

References 15 publications

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“…During preparation of the membrane it was shown that the molecular weight of DS was crucial in reaction and stability of the membrane. DS, 100,000 MW was used and this was in agreement to results reported by Berger et al, [8] Preparation of membrane was also dependent on volume, pH and temperature of solutions [13].…”

Section: Discussionsupporting

confidence: 84%

“…Biologically active HGF shows binding affinity to HSPG in SPR system and the affinity is decreased after addition of sulphated oligosaccharides such as DS to the samples [6,7]. Biosensor coated with DS was recently introduced for detection of HGF in cellular medium [8]. HGF as a potential therapeutic strategy in treatment of chronic inflammatory bowel diseases has been discussed in recent studies [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…Taking advantage of previous studies and observations, an experimental model has been established and presented using agarose membrane containing DS sodium salt that changes color to pink in contact with methylene blue [7,8]. Incubation of this membrane in feces in 10 seconds might distinguish samples containing active HGF that binds to DS and inhibits metachromasy.…”

Section: Introductionmentioning

confidence: 99%

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A methachromatic-based experimental model for identification of bowel as the focus of an acute inflammation

Nakka¹,

Johansson²,

Shahzad³

et al. 2013

OJGas

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Diarrhea is the most common symptom of acute inflammation in gastrointestinal tract and the patients are isolated in order to inhibit transmission and to conduct investigations. Yet there is no standard test to distinguish gastrointestinal infection from more generalized diseases at admittance which might cause delay in therapy. Hepatocyte growth factor (HGF) is produced upon injury by mesenchymal cells. On the contrary to chronic inflammation, HGF produced in the course of acute inflammation is biologically active and shows binding affinity to heparan sulphate proteoglycan (HSPG) and dextran sulphate (DS). Based on this phenomenon, an agarose gel containing DS was prepared and immobilized on loops to investigate the feces samples for the presence or absence of growth factors such as HGF with affinity to DS. The study is conducted as a clinical evaluation of an experimental model to distinguish acute infectious gastroenteritis from other causes of diarrhea. 656 fecal samples gathered consequently from patients seeking for bowel disturbances and healthy were tested by the test and the medical reports were investigated. Upon interaction with DS, methylene blue changes color to pink. This phenomenon was inhibited by HGF and converted by addition of anti-HGF antibodies to the samples. The test distinguished acute infectious gastroenteritis with high sensitivity and specificity (96% and 92% respectively) from other causes of diarrhea. We introduce a metachromatic experimental model that might distinguish acute inflammation in alimentary tract from other causes of diarrhea. This model might be used in developing rapid diagnostic tests.

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Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A methachromatic-based experimental model for identification of bowel as the focus of an acute inflammation

Nakka¹,

Johansson²,

Shahzad³

et al. 2013

OJGas

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“…Based on this principle, SAW sensors were initially developed more than three decades ago 25,26 , and are currently employed as fundamental components in a variety of microsensing systems. 8,18 The main advantage associated with SAW sensors is their high sensitivity and capability for the trace detection of a wide variety of chemical materials including bio/chemicals 27,28 , organic and inorganic vapors 29,30 , and explosives. 31 Furthermore, with continuous advancements in modern fabrication techniques, the development of miniaturized SAW sensors at relatively low costs is now possible.…”

Section: Saw-based Sensorsmentioning

confidence: 99%

Surface acoustic wave devices for chemical sensing and microfluidics: a review and perspective

et al. 2017

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Surface acoustic waves (SAWs), are electro-mechanical waves that form on the surface of piezoelectric crystals. Because they are easy to construct and operate, SAW devices have proven to be versatile and powerful platforms for either direct chemical sensing or for upstream microfluidic processing and sample preparation. This review summarizes recent advances in the development of SAW devices for chemical sensing and analysis. The use of SAW techniques for chemical detection in both gaseous and liquid media is discussed, as well as recent fabrication advances that are pointing the way for the next generation of SAW sensors. Similarly, applications and progress in using SAW devices as microfluidic platforms are covered, ranging from atomization and mixing to new approaches to lysing and cell adhesion studies. Finally, potential new directions and perspectives on the field as it moves forward are offered, with a specific focus on potential strategies for making SAW technologies for bioanalytical applications.

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“…In SPR systems, biologically active HGF exhibits binding affinity for HSPG [17], which is decreased after the addition of sulphated oligosaccharides, such as the glycosaminoglycan/dextran sulphate [18]. In addition, biosensors coated with dextran sulphate allow the detection of HGF in cellular medium [19]. Therefore, studying the presence and quality of HGF during different inflammatory processes may be useful [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Differences in the expression of hepatocyte growth factor in acute and chronic bowel inflammation—Implications for diagnosis?

Lönn¹,

Nakka²,

Olsson³

et al. 2013

ABB

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Background: Hepatocyte growth factor (HGF) acts as an acute phase protein with regenerative properties. HGF is produced systemically and locally during inflammation but exhibits decreased binding affinity to heparan sulphate proteoglycan (HSPG)/glycosaminoglycan during chronic inflammation. We previously observed a high faecal concentration and binding affinity of HGF to HSPG during acute gastroenteritis. High faecal concentrations of calprotectin and HGF have been reported in chronic inflammatory bowel disease (IBD). Methods: Stool samples from patients with ulcerative colitis in remission (n = 11) or exacerbation (n = 5), microscopic colitis (n = 11), colon cancer (n = 6), or acute gastroenteritis caused by Clostridium difficile (n = 20), as well as healthy controls (n = 7), were analysed for the presence of HGF by ELISA, surface plasmon resonance, SDS-PAGE, and Western blot. Then in two patients with ulcerative colitis exacerbation and C. difficile infection, the expression of HGF and calprotectin was studied in colonic biopsies. Results: The faecal concentration of HGF was significantly higher in patients with ulcerative colitis compared to the other groups. The binding affinity to dextran was lower in all groups compared to acute inflammation. HGF receptor binding was similar across groups. In a patient with concomitant C. difficile infection and distal ulcerative colitis, HGF was highly expressed in the part of the bowel unaffected by ulcerative colitis, but no expression was found at the site of chronic inflammation. In the patient with total colitis the biopsies showed low expression of HGF. The areas with chronic inflammation exhibited infiltrating calprotectin-stained neutrophils. Conclusion: HGF is produced locally during inflammation of the bowel. The HGF produced during acute inflammation or exacerbations of chronic inflammation by the unaffected area shows binding affinity to glucosaminoglycans. Measuring HGF binding in faeces and biopsies may be a tool for differentiating between acute and chronic bowel inflammation, which should be assessed thoroughly in future studies.

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Biosensors coated with sulfated polysaccharides for the detection of hepatocyte growth factor/scatter factor in cell culture medium

Cited by 14 publications

References 15 publications

A methachromatic-based experimental model for identification of bowel as the focus of an acute inflammation

A methachromatic-based experimental model for identification of bowel as the focus of an acute inflammation

Surface acoustic wave devices for chemical sensing and microfluidics: a review and perspective

Differences in the expression of hepatocyte growth factor in acute and chronic bowel inflammation—Implications for diagnosis?

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