Biosensoren auf der Basis von Katalasemembranen unter Nutzung der enzymatischen Doppelfunktion zur molekularselektiven Carrier-Analyse von Ethanol

Abstract

Biosensors Based on Catalase Membranes with Utilization of Enzymatic Bifunctionality for Molecular‐selective Carrier‐Analysis of Ethanol A robust bioelectrochemical measuring system for amperometric electroanalysis of ethanol is presented, which allows measurements between 0.01 and 99.8 vol.% of ethanol without preceeding dilution. It is distinguished by long‐lived biosensors based on bifunctional catalase membranes. Besides the O2‐sensitive enzymatic biosensor the design includes components for intermediate carrier analysis such as a microdialysator for analyte uptake into buffered, H2O2 containing carrier solution out of the sample solution both with tangential countercurrent fluxes, an oxigenating pump for constant pO2 in the carrier stream, a pressure adjusting chamber as protection from variing pressure on the enzyme membrane resulting from the roller pump's pulsations. Utilization of the membrane's catalatic and peroxidatic bifunctionality is the basis of ethanol analysis: peroxidatically acting catalase molecules cannot release oxygen by catalatic reaction of H2O2 at the same time.