Biosensor performance of phenol analysis using microbial consortium of Bacillus sp. and Pseudomonas sp.

Mulyawan, Reza; Iswantini, Dyah; Nurhidayat, Novik; Saprudin, Deden; Purwaningsih, Henny

doi:10.1063/5.0104743

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…The confirmation test results for the LoD value of 0.01% yielded a positive response, while the confirmed LoQ value of 0.02% showed good precision and accuracy. The low LoD and LoQ values, specifically 1% can be used to detect ethanol in food and beverages (Mulyawan et al 2022).…”

Section: Resultsmentioning

confidence: 99%

The analytical performance of Saccharomyces cerevisiae and Bacillus megaterium microbial consortium as recognition element in ethanol biosensor

NURDIANI,

ISWANTINI,

NURHIDAYAT

et al. 2023

Biodiversitas

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Abstract. Nurdiani, Iswantini D, Nurhidayat N, Wahyuni WT, Kartono A. 2023. The analytical performance of Saccharomyces cerevisiae and Bacillus megaterium microbial consortium as recognition element in ethanol biosensor. Biodiversitas 24: 5928-5936. Alcohol, particularly ethanol, is commonly found in human food and plays a significant role in degenerative diseases and disability. Accurate measurement of alcohol in food products is essential to ensure adherence to the Muslim halal rule. However, existing alcohol biosensor that relies on a single microbe has limitations in measuring a wide range of ethanol concentrations. To address this issue, a microbial consortium is needed to expand the measurable range. Therefore, this study aimed to develop an innovative biosensor to widen the range of measured ethanol concentrations based on the microbial consortium of Saccharomyces cerevisiae YSAPMI.2 and Bacillus megaterium BSAPMI.1. The performance of the biosensor was evaluated using the cyclic voltammetry method. The results showed that the linear range, linearity, coefficient of determination, sensitivity, and response time, were 0.02-6.0%, 0.9968, 0.9936, 83.157 µA (%)-1, and 11 seconds. The LoD ??and LoQ theoretical values ??of the method obtained in the ethanol oxidation reaction were 0.060% and 0.182%, respectively. The confirmatory test for the LoD value of 0.01% yielded a positive response, while the confirmed LoQ value of 0.02% showed good precision and accuracy. The biosensor had precise %RSD values of 0.568, 1.338, and 4.632% for the high, medium, and low ethanol concentrations, respectively. The accuracy reflected as the recovery percentage was in the range of 90.27-111.07%. The biosensor was relatively specific and had no interferences with common ethanol compounds including methanol, sodium chloride, formic acid, and glucose. The stability obtained with biofilm showed a better result reaching 88% in 70 days. Based on the result, this microbial consortium biosensor could widen the range of measured ethanol concentrations and should be further developed to create a prototype for an accurate and practical analysis.

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Section: Resultsmentioning

confidence: 99%

The analytical performance of Saccharomyces cerevisiae and Bacillus megaterium microbial consortium as recognition element in ethanol biosensor

NURDIANI,

ISWANTINI,

NURHIDAYAT

et al. 2023

Biodiversitas

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Bioanalytical System for Determining the Phenol Index Based on Pseudomonas putida BS394(pBS216) Bacteria Immobilized in a Redox-Active Biocompatible Composite Polymer “Bovine Serum Albumin–Ferrocene–Carbon Nanotubes”

et al. 2022

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The possibility of using the microorganisms Pseudomonas sp. 7p-81, Pseudomonas putida BS394(pBS216), Rhodococcus erythropolis s67, Rhodococcus pyridinivorans 5Ap, Rhodococcus erythropolis X5, Rhodococcus pyridinivorans F5 and Pseudomonas veronii DSM 11331T as the basis of a biosensor for the phenol index to assess water environments was studied. The adaptation of microorganisms to phenol during growth was carried out to increase the selectivity of the analytical system. The most promising microorganisms for biosensor formation were the bacteria P. putida BS394(pBS216). Cells were immobilized in redox-active polymers based on bovine serum albumin modified by ferrocenecarboxaldehyde and based on a composite with a carbon nanotube to increase sensitivity. The rate constants of the interaction of the redox-active polymer and the composite based on it with the biomaterial were 193.8 and 502.8 dm3/(g·s) respectively. For the biosensor created using hydrogel bovine serum albumin-ferrocene-carbon nanotubes, the lower limit of the determined phenol concentrations was 1 × 10−3 mg/dm3, the sensitivity coefficient was (5.8 ± 0.2)∙10−3 μA·dm3/mg, Michaelis constant KM = 230 mg/dm3, the maximum rate of the enzymatic reaction Rmax = 217 µA and the long-term stability of the bioanalyzer was 11 days. As a result of approbation, it was found that the urban water phenol content differed insignificantly, measured by creating a biosensor and using the standard photometric method.

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Biosensor performance of phenol analysis using microbial consortium of Bacillus sp. and Pseudomonas sp.

Cited by 2 publications

References 12 publications

The analytical performance of Saccharomyces cerevisiae and Bacillus megaterium microbial consortium as recognition element in ethanol biosensor

The analytical performance of Saccharomyces cerevisiae and Bacillus megaterium microbial consortium as recognition element in ethanol biosensor

Bioanalytical System for Determining the Phenol Index Based on Pseudomonas putida BS394(pBS216) Bacteria Immobilized in a Redox-Active Biocompatible Composite Polymer “Bovine Serum Albumin–Ferrocene–Carbon Nanotubes”

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