Biosensor for the Nonspecific Determination of Ionic Surfactants

Abstract

We report the analytical figures of merit for the first biosensor for ionic surfactant quantification. The biosensor consists of a silanized silica optical fiber, onto which acrylodan-labeled bovine serum albumin (BSA-Ac) is immobilized. Recent work from our laboratory on native BSA-Ac (Lundgren, J. S.; Bright, F. V. J. Phys. Chem. 1996, 100, 8580-8586) has shown that ionic surfactants dehydrate the local environment surrounding the lone acrylodan residue, open up the pocket hosting the acrylodan reporter group, and dramatically increase the segmental mobility of domain I in BSA. In the current work, we use BSA-Ac as an actual biorecognition element for surfactant detection and quantification. We also compare several BSA-Ac immobilization strategies and determine the analytical figures of merit for the BSA-Ac-based biosensor to a prototypical analyte, cetyltrimethylammonium bromide (CTAB). The biosensor linear dynamic range extends from 5 to 60 μM, and the t(90) response time (90% of the total response) is less than 30 s. Biosensor response precision (relative standard deviation) during 34 sensing cycles is 2.5%. On the down side, biosensor performance decreases 38% after 25 days of storage; however, this drift can be compensated. This work also demonstrates the utility of BSA-Ac as a model biorecognition element-reporter group system for grading the suitability of different surface immobilization strategies.

Metal–Organic Framework as a Chemosensor Based on Luminescence Properties for Monitoring Cetyltrimethylammonium Bromide and Its Application in Smartphones

“…In all surfactants, cetyltrimethylammonium bromide (CTAB) is an important representative of quaternary ammonium salt cationic surfactants . Up to now, several approaches, such as mass spectrometry, capillary electrophoresis, colorimetry, , electrochemistry, liquid chromatography–mass spectrometry, and fluorimetry, − have been established to detect CTAB. Among them, fluorescent sensors have attracted a large number of interests for the simple operation, low cost, requisite selectivity, and high sensitivity.…”

Optical Sensor of Anionic Surfactants Using Solid-Phase Extraction with a Lactone-form Rhodamine B Membrane

“…An optical detection of cationic surfactants using an environment-sensitive fluorophore, 5-[(2-aminoethyl)amino]naphthalene-1-sulfonate, has been reported by Seitz et al 11 The optical sensor responds to a cationic surfactant in the concentration range from 0.1 to 1 mM (M = mol dm -3 ). A cationic surfactant biosensor reported by Lundgren et al 12 uses acrylodan-labeled bovine serum albumin as the biorecognition element. The biosensor responds to cationic surfactants in the concentration range from 5 to 60 mM.…”

Optical Sensing Membrane Based on Tetrabromophenolphthalein Ethyl Ester for the Determination of Cationic Surfactants

“…However, the sensitivity of the optical sensor is relatively low: the minimum detectable concentration is between 0.1 and 1 mM. A biosensor for the determination of ionic surfactant has been reported (Lundgren et al 1996). They use acrylodan-labeled bovine serum albumin (BSA-Ac) as the biorecognition element to detect certain ionic surfactant such as cetyltrimethylammonium bromide in the concentration range from 5 to 60 mM.…”