Biosensor Based on Ultrasmall MoS2 Nanoparticles for Electrochemical Detection of H2O2 Released by Cells at the Nanomolar Level

Wang, Tanyuan; Zhu, Haichuan; Zhuo, Junqiao; Zhu, Zhiwei; Papakonstantinou, Pagona; Lubarsky, Gennady; Lin, Jian; Li, Meixian

doi:10.1021/ac402114c

Cited by 446 publications

(252 citation statements)

References 60 publications

(83 reference statements)

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“…7C), the applied potential for amperometric sensing of H 2 O 2 should be more positive to decrease the background current and minimize the response of common interference species. 26,27 We studied the influence of the applied potential on the amperometric response of the sensor to H 2 O 2 . As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Hemin Modified TiO₂Nanoparticles with Enhanced Photoelectrocatalytic Activity for Electrochemical and Photoelectrochemical Sensing

Zhu

Yan

et al. 2016

J. Electrochem. Soc.

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Hemin-functionalized TiO 2 nanoparticles were prepared and assembled on glassy carbon electrode through the electrostatic attraction between positively charged poly(diallyldimethylammonium chloride) and negatively charged hemin and TiO 2 . The scanning electron microscopy and X-ray diffraction characterization showed that the assembled hemin-TiO 2 film displayed a mesoporous structure comprising plenty of TiO 2 nanoparticles in rutile phase. The Fourier transform infrared spectra and UV-visible diffuse reflectance spectra indicated that hemin was successfully incorporated into TiO 2 film and significantly enhanced the absorption of TiO 2 film to visible light. The hemin-TiO 2 film exhibited a pair of well-defined redox peaks of hemin by cyclic voltammetry, which could effectively catalyze the electroreduction of H 2 O 2 . Thus, the hemin-TiO 2 modified electrode was employed as an electrochemical sensor for H 2 O 2 determination, showing a sensitive response linearly proportional to the concentration of H 2 O 2 from 3.0 × 10 −7 to 4.7 × 10 −4 mol · L −1 . At the same time, the photoelectrocatalytic activity of TiO 2 under visible light illumination was dramatically promoted by hemin. The hemin-TiO 2 modified electrode produced a photoelectrochemical response linearly proportional to hydroquinone in the concentration range from 4. Hemin is an iron-containing porphyrin, namely protoporphyrin IX Fe(III) complex, which is well-known as the active center of the hemeprotein family. Because of the reversible reaction of Fe(III)/Fe(II) redox couple, hemin has been extensively demonstrated as an efficient electrocatalyst for many small molecules such as hydrogen peroxide, 1-3 oxygen, 4 nitrite, 5 L-tyrosine 6 and artemisinin. 7 Generally, hemin is loaded on some supporting materials to avoid the molecular aggregation of hemin molecules in aqueous solution and improve the stability and activity of catalyst.8 On the other hand, hemin is a structural analogue of chlorophyll, which can serve as a promising photosensitizer for TiO 2 photocatalyst to harvest visible light. 9,10 Moreover, the presence of Fe(III) porphyrin ring on the surface of TiO 2 can reduce the electron-hole recombination rate and act as a mediator for continuous production of enriched concentration of hydroxyl radicals to enhance the photocatalytic activity of TiO 2 . 11TiO 2 is the most intensively studied photocatalyst for degradation of various organic pollutants.12 In recent years, TiO 2 nanomaterials have been widely utilized for fabrication of sensing devices because of their fascinating properties such as large surface area, good biocompatibility, high stability, and unique electronic and photocatalytic performances.13 When catalytic materials including metal nanoparticles, small molecules and biological macromolecules are immobilized on nanostructured TiO 2 , the obtained nanocomposites can act as bifunctional catalysts which not only possess the catalytic activity of introduced materials but also preserve the intrinsic photocatalytic capacity of T...

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Section: Resultsmentioning

confidence: 99%

Hemin Modified TiO₂Nanoparticles with Enhanced Photoelectrocatalytic Activity for Electrochemical and Photoelectrochemical Sensing

Zhu

Yan

et al. 2016

J. Electrochem. Soc.

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“…The imbalance of GSH/ GSSG is connected with various diseases, including neurodegenerative disorders, human immunodeficiency virus (HIV), aging, and Alzheimer's disease [2,3]. Notably, hydrogen peroxide (H 2 O 2 ) is one of a series of reactive oxygen species (ROS) and a by-product of vast biological processes [4]. Based on the reducibility of GSH, it is envisaged that GSH could result in the reduction of the H 2 O 2 -3,3′,5,5′-tetramethylbenzidine (TMB) system.…”

Section: Introductionmentioning

confidence: 99%

Novel synthesis of NiS/MMT/GO nanocomposites with enhanced peroxidase-like activity for sensitive colorimetric detection of glutathione in solution

Chen

Zhu

Yang

et al. 2018

Adv Compos Hybrid Mater

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In the present study, a facile method was proposed to prepare nickel sulfide (NiS) nanoparticles well-dispersed on the surface of montmorillonite/graphene oxide (MMT/GO). The NiS/MMT/GO nanocomposites were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), fluorescence spectrophotometry, etc. Significantly, the novel NiS/MMT/GO nanocomposites exhibited high peroxidase-like activity toward the typical chromogenic substrate, 3,3′,5,5′-tetramethylbenzidine (TMB). However, glutathione (GSH) could inhibit the peroxidase-like activity of NiS/ MMT/GO along with a visible color variation. Based on the color change of the system of nanocomposites-H 2 O 2 -TMB, a novel colorimetric sensor was designed and conveniently used to quantitatively detect H 2 O 2 as well as GSH. The limit of detection (LOD) of H 2 O 2 and GSH is 9.73 and 0.5043 μM, respectively. The proposed H 2 O 2 sensor has potential application in the field of biosensing, food, and environment.

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“…Therefore, accurate and sensitive determination of H 2 O 2 has been a challenging requirement in recent years. So far, many methods have been developed for detection of H 2 O 2 concentration, including titrimetry [7], chromatography [8], spectrophotometry [9], and electrochemical method [10]. Among them, the electrochemical method has received more attention due to its high sensitivity, low cost, convenient operation, and fast response [11,12].…”

Section: Introductionmentioning

confidence: 99%

Carbon dots-decorated multiwalled carbon nanotubes nanocomposites as a high-performance electrochemical sensor for detection of H2O2 in living cells

2016

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A novel enzyme-free hydrogen peroxide sensor composed of carbon dots (CDs) and multi-walled carbon nanotubes (MWCNTs) was prepared. It was found that the carbon dots-decorated multi-walled carbon nanotubes nanocomposites (CDs/MWCNTs) modified glassy carbon (GC) electrode (CDs/MWCNTs/GCE) exhibited a significant synergistic electrocatalytic activity towards hydrogen peroxide reduction as compared to carbon dots or multi-walled carbon nanotubes alone, and the CDs/MWCNTs/GCE has shown a low detection limit as well as excellent stability, selectivity, and reproducibility. These remarkable analytical advantages enable the practical application of CDs/MWCNTs/GCE for the real-time tracking of hydrogen peroxide (H2O2) released from human cervical cancer cells with satisfactory results. The enhanced electrochemical activity can be assigned to the edge plane-like defective sites and lattice oxygen in the CDs/MWCNTs nanocomposites due to the small amount of decoration of carbon dots on the multi-walled carbon nanotubes. Based on a facile preparation method and with good electrochemical properties, the CDs/MWCNTs nanocomposites represent a new class of carbon electrode for electrochemical sensor applications. Graphical Abstract CDs/MWCNTs exhibited good electrocatalytic activity and stability to H2O2 reduction and can be used for real-time detection of H2O2 released from living cells.

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Biosensor Based on Ultrasmall MoS₂ Nanoparticles for Electrochemical Detection of H₂O₂ Released by Cells at the Nanomolar Level

Cited by 446 publications

References 60 publications

Hemin Modified TiO₂Nanoparticles with Enhanced Photoelectrocatalytic Activity for Electrochemical and Photoelectrochemical Sensing

Hemin Modified TiO₂Nanoparticles with Enhanced Photoelectrocatalytic Activity for Electrochemical and Photoelectrochemical Sensing

Novel synthesis of NiS/MMT/GO nanocomposites with enhanced peroxidase-like activity for sensitive colorimetric detection of glutathione in solution

Carbon dots-decorated multiwalled carbon nanotubes nanocomposites as a high-performance electrochemical sensor for detection of H2O2 in living cells

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Biosensor Based on Ultrasmall MoS2 Nanoparticles for Electrochemical Detection of H2O2 Released by Cells at the Nanomolar Level

Cited by 446 publications

References 60 publications

Hemin Modified TiO2Nanoparticles with Enhanced Photoelectrocatalytic Activity for Electrochemical and Photoelectrochemical Sensing

Hemin Modified TiO2Nanoparticles with Enhanced Photoelectrocatalytic Activity for Electrochemical and Photoelectrochemical Sensing

Novel synthesis of NiS/MMT/GO nanocomposites with enhanced peroxidase-like activity for sensitive colorimetric detection of glutathione in solution

Carbon dots-decorated multiwalled carbon nanotubes nanocomposites as a high-performance electrochemical sensor for detection of H2O2 in living cells

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Biosensor Based on Ultrasmall MoS₂ Nanoparticles for Electrochemical Detection of H₂O₂ Released by Cells at the Nanomolar Level

Hemin Modified TiO₂Nanoparticles with Enhanced Photoelectrocatalytic Activity for Electrochemical and Photoelectrochemical Sensing

Hemin Modified TiO₂Nanoparticles with Enhanced Photoelectrocatalytic Activity for Electrochemical and Photoelectrochemical Sensing