Biosensor based on hydrogel optical waveguide spectroscopy for the detection of 17β-estradiol

Potassium Acyltrifluoroborate (KAT) Ligations are Orthogonal to Thiol‐Michael and SPAAC Reactions: Covalent Dual Immobilization of Proteins onto Synthetic PEG Hydrogels

“…Hydrogels are important biomaterials for a variety of applications such as biosensors, drug delivery, and 3D cell culture . Their high water content and mass transport properties mimic physiological conditions and can provide, to a first approximation, a satisfying system to mimic the extracellular matrix (ECM) and allow the encapsulation and culture of cells.…”

Asymmetric split H-shape nanoantennas for molecular sensing

“…These techniques, such as screen-printed carbon electrochemistry (SPCE) [23], gas chromatography-mass spectrometry (GC-MS) [24], gold electrode surface via under-potential deposition (UDP) [25] and aptamer-based optical fiber [26], give a modest limit of detection (LOD) and require complex experimental laboratory work. Recent development of the SEIRA method by using plasmonic resonance from nanostructures has been shown to be rapid and simple for a specific assay of 17β-estradiol [31,32]. However, the approach used excluded the evaluation of the molecular-bond vibrational resonance signature in the mid-infrared region that is exhibited by E2, with LOD values on the nanomolar scale [26, 31–39].…”

“…Recent development of the SEIRA method by using plasmonic resonance from nanostructures has been shown to be rapid and simple for a specific assay of 17β-estradiol [31,32]. However, the approach used excluded the evaluation of the molecular-bond vibrational resonance signature in the mid-infrared region that is exhibited by E2, with LOD values on the nanomolar scale [26, 31–39]. In this study, we show that with the new ASH structure, zeptomole LOD values can be achieved through evaluation of the C-H vibrational resonance exhibited by E2.…”

Sensors based on surface plasmon resonance in a plastic optical fiber for the detection of trinitrotoluene

“…As a proof of principle, 2,3,6-trinitrotoluene (TNT), an aromatic nitroderivative with low molecular mass, has been considered. SPR biosensors are commonly regarded as suitable for the analysis of medium or large molecular weight analytes, however several approaches have been proposed for the detection of low molecular mass substances [11,12] too, mainly based on reactions which give reaction products inducing measurable refractive index changes upon binding on the surface of the optical device. Another aspect to be considered is that the use of an optical fiber, differently from the commercially available devices, makes the remote sensing straightforward, and may reduce the cost and dimension of the device, with the possibility of integration of SPR sensing platform with optoelectronic devices, eventually leading to "Lab-on-a-chip".…”