Biosensor applications in contaminated estuaries: Implications for disaster research response

Camargo, Krisa; Vogelbein, Mary Ann; Horney, Jennifer A.; Dellapenna, Timothy M.; Knap, Anthony H.; Sericano, José L.; Wade, Terry L.; McDonald, Thomas J.; Chiu, Weihsueh A.; Unger, Michael A.

doi:10.1016/j.envres.2021.111893

Cited by 7 publications

(4 citation statements)

References 59 publications

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“…The KinExA-based assays have proven to be highly sensitive and versatile in detecting and quantifying various molecules of interest across a wide range of applications [ [40] , [44] , [45] , [46] , [47] , [48] , [49] , [50] , [51] , [52] ]. These molecules encompass bacteria [ 44 ], chemotherapeutic drugs targeting tyrosine kinase inhibitors [ 45 ], therapeutic monoclonal antibodies [ 46 ], hormones [ 47 ], biomarkers associated with different diseases and conditions [ 40 ], opiates [ 48 ], environmental contaminants [ 49 , 50 ], food toxins [ 51 ], and heavy metals [ 52 ].…”

Section: Resultsmentioning

confidence: 99%

Novel ultrasensitive automated kinetic exclusion assay for measurement of plasma levels of soluble PD-L1, the predictive and prognostic biomarker in cancer patients treated with immune checkpoint inhibitors

Darwish,

Alahmad,

Vinoth

2024

Heliyon

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Section: Resultsmentioning

confidence: 99%

Novel ultrasensitive automated kinetic exclusion assay for measurement of plasma levels of soluble PD-L1, the predictive and prognostic biomarker in cancer patients treated with immune checkpoint inhibitors

Darwish,

Alahmad,

Vinoth

2024

Heliyon

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“…In collaboration with an SRP-funded researcher from the Virginia Institute of Marine Sciences, TAMU scientists field-tested a sophisticated biosensor [ 58 ], demonstrating its utility for quickly characterizing and prioritizing environmental samples for further analysis [ 59 ]. This tool may be particularly useful in the context of disaster research response where time and resources are limited.…”

Section: Understanding Contaminant Movement In a Changing Climatementioning

confidence: 99%

Adapting to Climate Change: Leveraging Systems-Focused Multidisciplinary Research to Promote Resilience

Amolegbe

Lopez

Velasco

et al. 2022

IJERPH

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Approximately 2000 official and potential Superfund sites are located within 25 miles of the East or Gulf coasts, many of which will be at risk of flooding as sea levels rise. More than 60 million people across the United States live within 3 miles of a Superfund site. Disentangling multifaceted environmental health problems compounded by climate change requires a multidisciplinary systems approach to inform better strategies to prevent or reduce exposures and protect human health. The purpose of this minireview is to present the National Institute of Environmental Health Sciences Superfund Research Program (SRP) as a useful model of how this systems approach can help overcome the challenges of climate change while providing flexibility to pivot to additional needs as they arise. It also highlights broad-ranging SRP-funded research and tools that can be used to promote health and resilience to climate change in diverse contexts.

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“…The mAb 2G8 antibody was initially developed for use on the KinExA Inline Sensor (Sapidyne Instruments), a biosensing instrument that quantifies PAH concentration via the detection of fluorescently tagged antibody bound to PAHs in an aqueous sample (Li et al, 2016 ). Employing mAb 2G8 conjugated to the far‐red fluorescent dye Alexa Fluor 647 (AF647), the biosensor has been successfully used to quantify total three‐ to five‐ring PAH concentrations in environmental samples such as porewater (Camargo et al, 2022 ; Conder et al, 2021 ; Hartzell et al, 2017 ; Li et al, 2016 ) and oyster interstitial fluid (Prossner et al, 2022 ). Extending beyond its employment as a quantitative tool, we explore a novel application of AF647‐tagged mAb 2G8 to observe PAH localization in oyster gill tissues using IHC techniques coupled with confocal microscopic imaging.…”

Section: Introductionmentioning

confidence: 99%

Immunofluorescence Visualization of Polycyclic Aromatic Hydrocarbon Mixtures in the Eastern Oyster Crassostrea virginica

Prossner¹,

Small²,

Carnegie³

et al. 2023

Enviro Toxic and Chemistry

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Bivalve mollusks including oysters have low metabolic potential and are therefore susceptible to accumulating high levels of lipophilic organic contaminants such as polycyclic aromatic hydrocarbons (PAHs). Human exposure to PAHs via consumption of this important commercial shellfish can be a serious public health concern in areas where high PAH contamination exists. Previous PAH immunohistochemical studies have been limited to laboratory‐based exposures focusing on one or a few individual PAH compounds. To date, such studies have yet to explore PAH accumulation in oysters, known to have some of the highest levels of PAHs across different food products. Using a monoclonal antibody selective for a range of three‐ to five‐ring PAHs, we present a method to detect and localize complex mixtures of PAHs in oyster tissues via fluorescent immunohistochemistry. Observed immunofluorescence intensity followed a similar trend as measured levels of PAHs in oyster interstitial fluid from PAH‐contaminated sites and oysters exposed to the water accommodated fraction of crude oil. This method will be valuable in understanding internal partitioning mechanisms of PAH‐exposed oysters and will have important applications in studies on PAH distribution in the tissues of additional organisms for environmental, medical, or veterinary purposes. Environ Toxicol Chem 2023;42:475–480. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

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Biosensor applications in contaminated estuaries: Implications for disaster research response

Cited by 7 publications

References 59 publications

Novel ultrasensitive automated kinetic exclusion assay for measurement of plasma levels of soluble PD-L1, the predictive and prognostic biomarker in cancer patients treated with immune checkpoint inhibitors

Novel ultrasensitive automated kinetic exclusion assay for measurement of plasma levels of soluble PD-L1, the predictive and prognostic biomarker in cancer patients treated with immune checkpoint inhibitors

Adapting to Climate Change: Leveraging Systems-Focused Multidisciplinary Research to Promote Resilience

Immunofluorescence Visualization of Polycyclic Aromatic Hydrocarbon Mixtures in the Eastern Oyster Crassostrea virginica

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