Biosensor Application of Carbonaceous Nanocoil Material: Preparation, Characterization, and Determination of Dopamine and Uric Acid in the Presence of Ascorbic Acid

Abstract

Carbonized electrode materials were prepared from carbonization of graphene nanoribbon (GNR) / coal tar pitch (CTP) composite (m% of GNR: 1%, 3%, 5% and 10%). The carbonized materials were denoted as follows: CTP-GNR1, CTP-GNR2, CTP-GNR3 and CTP-GNR4. Characterization included cyclic voltammetry (CV), infrared spectroscopy (IR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and X-ray diffraction (XRD). CTP-GNR1 was obtained as the nanocoil structure via SEM. Electrocatalytic activity for some redox probes, such as ferrocene and ferric/ferrous (K3Fe(CN)6 and K4Fe(CN)6) probe on the CTP-GNR1 modified glassy carbon (GC/CTP-GNR1) was found to be greater than others. The nanocoil material (CTP-GNR1) was used in the determination of dopamine (DA) and uric acid (UA) by differential pulse voltammetry (DPV). The electrode exhibits linear responses to DA and UA in the ranges of 2 μM–20 μM in the presence of 300 μM of L-ascorbic acid (AA). The detection limits (S/N = 3) for DA and UA are 3 nM and 6 nM, respectively.

Electrochemical Characterization of Carbonized Typha Tassel Modified ScreenPrinted Electrode and Its Enzymatic Glucose Oxidation Application

“…Figure 5 (A) shows the electrochemical performance of 1 mg/cm 2 coated CTT electrode using CV at various scan rates in 0.1 M PBS solution containing 1 mM FcCOOH at pH 7.4. Anodic and cathodic peaks have been obtained around 150 mV and 50 mV vs Ag/Ag+ respectively with an oxidation onset potential of around 25 mV, which is in parallel with previously reported redox potentials for ferrocene [24]. The fast, efficient and diffusion-controlled electron transfer processes have been confirmed with the linear relationship between peak current and square root scan as shown in Figure 5 (B).…”

“…CTT were prepared using a method published previously [24]. Briefly, 10 g of TT was carbonized in a tube furnace at 1000°C under flowing inert nitrogen at a heating ramp of 10°C/min for 1 h. After the carbonization procedure, SEM images were taken and FTIR analyses were performed for RTT and CTT.…”

Progress in Carbon Nanotube-Based Electrochemical Biosensors – A Review

“…Moreover, the modified electrode also provides good sensitivity and selectivity for the determination of DA, UA and Try. Erkal et al (2016) described the development of a novel biosensor for simultaneous determination of dopamine, uric acid, and folic acid by carbon paste electrode modified by hemoglobin which is electrostatically immobilised on silica-coated magnetic nanoparticles and MWCNTs. The modified carbon paste electrode provided a sensitive and stable biosensor for simultaneous determination of dopamine, uric acid, and folic acid whose performance is much better than those of many previously reported sensors.…”

An electrochemical label-free DNA impedimetric sensor with AuNP-modified glass fiber/carbonaceous electrode for the detection of HIV-1 DNA

“…However, CNTs and GPH are some of the expensive examples of CMs; on the other hand, low-cost CMs can also be synthesized using low-cost alternatives such as coal tar pitch (CTP). Carbonized CTP can be a very good alternative with high surface area and excellent electrical conductivity especially for the synthesis of CTP composites (Erkal et al 2016 ). GF is a low-cost and commercially available material often used in refractor materials and furnace insulations due to aluminum silicate in the structure; therefore, its use in bioelectronic applications is limited.…”