Biosensing Using SERS Active Gold Nanostructures

Das, Gour Mohan; Managò, Stefano; Mangini, Maria; Luca, Anna Chiara De

doi:10.3390/nano11102679

Cited by 45 publications

(35 citation statements)

References 124 publications

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“…In recent years, SERS technology has been applied to the study of exosome detection, which has greatly improved the sensitivity of exosome detection, which is significant for the development of new liquid biopsy techniques based on exosomes ( Lee et al, 2020 ; Han et al, 2021c ; Das et al, 2021 ; Fan et al, 2021 ; Guerrini et al, 2021 ). The forms of exosome detection by SERS technology are mainly divided into non-labeled and labeled assays.…”

Section: Future Perspections and Discussionmentioning

confidence: 99%

Advances in the Application of Exosomes Identification Using Surface-Enhanced Raman Spectroscopy for the Early Detection of Cancers

Yang

Jia

2022

Front. Bioeng. Biotechnol.

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Exosomes are small nanoscale vesicles with a double-layered lipid membrane structure secreted by cells, and almost all types of cells can secrete exosomes. Exosomes carry a variety of biologically active contents such as nucleic acids and proteins, and play an important role not only in intercellular information exchange and signal transduction, but also in various pathophysiological processes in the human body. Surface-enhanced Raman Spectroscopy (SERS) uses light to interact with nanostructured materials such as gold and silver to produce a strong surface plasmon resonance effect, which can significantly enhance the Raman signal of molecules adsorbed on the surface of nanostructures to obtain a rich fingerprint of the sample itself or Raman probe molecules with ultra-sensitivity. The unique advantages of SERS, such as non-invasive and high sensitivity, good selectivity, fast analysis speed, and low water interference, make it a promising technology for life science and clinical testing applications. In this paper, we briefly introduce exosomes and the current main detection methods. We also describe the basic principles of SERS and the progress of the application of unlabeled and labeled SERS in exosome detection. This paper also summarizes the value of SERS-based exosome assays for early tumor diagnosis.

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Section: Future Perspections and Discussionmentioning

confidence: 99%

Advances in the Application of Exosomes Identification Using Surface-Enhanced Raman Spectroscopy for the Early Detection of Cancers

Yang

Jia

2022

Front. Bioeng. Biotechnol.

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“…The strong optical scattering characteristics and relative histocompatibility of AuNPs are also used to detect tumour markers. In surface-enhanced Raman spectroscopy (SERS), AuNPs can amplify the Raman signal of the detection molecules several times; Thus, AuNPs are the most popular nanostructures used as sensors presently( Das et al, 2021 ). Under near infrared (NIR) irradiation, AuNPs show extremely strong light-heat conversion efficiency, which increases the local temperature of the tumour site, leading to apoptosis of the tumour cells that are extremely sensitive to heat( Singh et al, 2018 ).…”

Section: Unique Characterization Of Aunps and Their Applicability In ...mentioning

confidence: 99%

Aptamer-conjugated gold nanoparticles and their diagnostic and therapeutic roles in cancer

Deng

Luo

et al. 2023

Front. Bioeng. Biotechnol.

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The burden of incidence rate and mortality of cancer is increasing rapidly, and the development of precise intervention measures for cancer detection and treatment will help reduce the burden and pain of cancer. At present, the sensitivity and specificity of tumor markers such as CEA and CA-125 used clinically are low, while PET, SPECT, and other imaging diagnoses with high sensitivity possess shortcomings, including long durations to obtain formal reports and the inability to identify the molecular pathological type of cancer. Cancer surgery is limited by stage and easy to recur. Radiotherapy and chemotherapy often cause damage to normal tissues, leading to evident side effects. Aptamers can selectively and exclusively bind to biomarkers and have, therefore, gained attention as ligands to be targeted for cancer detection and treatment. Gold nanoparticles (AuNPs) are considered as promising nano carriers for cancer diagnosis and treatment due to their strong light scattering characteristics, effective biocompatibility, and easy surface modification with targeted agents. The aptamer-gold nanoparticles targeting delivery system developed herein can combine the advantages of aptamers and gold nanoparticles, and shows excellent targeting, high specificity, low immunogenicity, minor side effects, etc., which builds a bridge for cancer markers to be used in early and efficient diagnosis and precise treatment. In this review, we summarize the latest progress in the application of aptamer-modified gold nanoparticles in cancer targeted diagnosis and delivery of therapeutic agents to cancer cells and emphasize the prospects and challenges of transforming these studies into clinical applications.

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“…Thus, nanoparticles less than 100 nm in size are considered optimal for the fabrication of SERS substrate because when large nanoparticles are irradiated with a laser, multipole excitation arises, which is nonradiative and reduces enhancement efficiency. When choosing the shape of metal nanoparticles, preference is given to anisotropic structures, such as star-shaped nanoparticles, which, due to their unique optical properties, are widely used in biosensors and imaging techniques [ 94 ]. Of particular interest is the aggregation of colloidal metal nanoparticles, which provides a high EF.…”

Section: Application Of Sers and Sers-combined Raman Techniques In The Detection Of Biomoleculesmentioning

confidence: 99%

“…The formation of solid substrates is carried out by self-assembly [ 97 , 98 , 99 ] or lithography [ 100 , 101 , 102 ] methods or using the combination of these methods, resulting in the creation of substrates with different SPR properties. Table 1 summarizes various approaches to create SERS-active substrates [ 86 , 94 ]. The self-assembly is a cost-effective and rapid approach for creating a SERS-active solid substrate.…”

Section: Application Of Sers and Sers-combined Raman Techniques In The Detection Of Biomoleculesmentioning

confidence: 99%

Raman Scattering-Based Biosensing: New Prospects and Opportunities

et al. 2021

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The growing interest in the development of new platforms for the application of Raman spectroscopy techniques in biosensor technologies is driven by the potential of these techniques in identifying chemical compounds, as well as structural and functional features of biomolecules. The effect of Raman scattering is a result of inelastic light scattering processes, which lead to the emission of scattered light with a different frequency associated with molecular vibrations of the identified molecule. Spontaneous Raman scattering is usually weak, resulting in complexities with the separation of weak inelastically scattered light and intense Rayleigh scattering. These limitations have led to the development of various techniques for enhancing Raman scattering, including resonance Raman spectroscopy (RRS) and nonlinear Raman spectroscopy (coherent anti-Stokes Raman spectroscopy and stimulated Raman spectroscopy). Furthermore, the discovery of the phenomenon of enhanced Raman scattering near metallic nanostructures gave impetus to the development of the surface-enhanced Raman spectroscopy (SERS) as well as its combination with resonance Raman spectroscopy and nonlinear Raman spectroscopic techniques. The combination of nonlinear and resonant optical effects with metal substrates or nanoparticles can be used to increase speed, spatial resolution, and signal amplification in Raman spectroscopy, making these techniques promising for the analysis and characterization of biological samples. This review provides the main provisions of the listed Raman techniques and the advantages and limitations present when applied to life sciences research. The recent advances in SERS and SERS-combined techniques are summarized, such as SERRS, SE-CARS, and SE-SRS for bioimaging and the biosensing of molecules, which form the basis for potential future applications of these techniques in biosensor technology. In addition, an overview is given of the main tools for success in the development of biosensors based on Raman spectroscopy techniques, which can be achieved by choosing one or a combination of the following approaches: (i) fabrication of a reproducible SERS substrate, (ii) synthesis of the SERS nanotag, and (iii) implementation of new platforms for on-site testing.

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Biosensing Using SERS Active Gold Nanostructures

Cited by 45 publications

References 124 publications

Advances in the Application of Exosomes Identification Using Surface-Enhanced Raman Spectroscopy for the Early Detection of Cancers

Advances in the Application of Exosomes Identification Using Surface-Enhanced Raman Spectroscopy for the Early Detection of Cancers

Aptamer-conjugated gold nanoparticles and their diagnostic and therapeutic roles in cancer

Raman Scattering-Based Biosensing: New Prospects and Opportunities

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