Biosensing Platforms Based on Graphene Field Effect Transistors

Abstract

Since ion-sensitive field effect transistors (ISFETs) have been developed in 1970, much attention has been focused on FET-based biosensors. In particular, graphene is one of the promising candidates for FET-based biosensors owing to their high electron/hole mobilities and unique 2D nature. Herein, we report specific detection of biomolecules using sialylglycopeptide-modified graphene FET (G-FET) biosensors. In addition, we also present state-space models (SSMs) to analyze time-series data of G-FET biosensors. This computational approach helps to separate the response against target biomolecules from the baseline drifted data. G-FET biosensors open the door to a more versatile sensing platform for biomolecules and biological functions.

Graphene Field-Effect Transistors with Surface-Charge Modulation for C-Reactive Protein Detection in Artificial Saliva

“…To circumvent these complexities, artificial saliva of known composition was utilized. Additionally, this study employed reference GFETs without capture probes alongside probe immobilized GFETs, conducting differential measurements to mitigate responses to environmental factors like pH and ionic strength variations 28,29 and nonspecific adsorption, thereby extracting net signals related to the target analyte. Additionally, to address the inherent FET-to-FET variability resulting from process variations in GFET characteristics, 30,31 multiple GFETs were integrated into the chips.…”