Biosensing in high-performance chemical separations

Trojanowicz, Marek; Szewczyńska, Małgorzata

doi:10.1016/j.trac.2004.11.008

Cited by 20 publications

(6 citation statements)

References 131 publications

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“…Several immobilization techniques would be suitable for the purpose of this work, but due to the simplicity and the availability of the materials, the enzyme was immobilized onto a solid carrier which was kept in the reactor, separate from the detector, and which could be easily removed and replaced with fresh active enzyme [15]. As a support for enzyme immobilization, controlled pore glass is the most often used material [16,26]. The enzyme cross-linked with glutaraldehyde [27] showed good operational and storage stability (the enzyme was stable during one day of operation and the enzyme activity remained unchanged over 6 months of storage [17]).…”

Section: Inhibition (%) [Concentration Of Inhibitor (M)]mentioning

confidence: 99%

Inhibition of AChE by malathion and some structurally similar compounds

Krstić

Čolović

Kralj

et al. 2008

Journal of Enzyme Inhibition and Medicinal Chemistry

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Inhibition of bovine erythrocyte acetylcholinesterase (free and immobilized on controlled pore glass) by separate and simultaneous exposure to malathion and malathion transformation products which are generally formed during storage or through natural or photochemical degradation was investigated. Increasing concentrations of malathion, its oxidation product malaoxon, and its isomerisation product isomalathion inhibited free and immobilized AChE in a concentration-dependent manner. K I , the dissociation constant for the initial reversible enzyme inhibitor-complex, and k 3 , the first order rate constant for the conversion of the reversible complex into the irreversibly inhibited enzyme, were determined from the progressive development of inhibition produced by reaction of native AChE with malathion, malaoxon and isomalathion. .2) £ 10 26 M, were obtained from the inhibition curves induced by malathion, malaoxon and isomalathion, respectively. However, the products formed due to photoinduced degradation, phosphorodithioic O,O,S-trimethyl ester and O,O-dimethyl thiophosphate, did not noticeably affect enzymatic activity, while diethyl maleate inhibited AChE activity at concentrations . 10 mM. Inhibition of acetylcholinesterase increased with the time of exposure to malathion and its inhibiting by-products within the interval from 0 to 5 minutes. Through simultaneous exposure of the enzyme to malaoxon and isomalathion, an additive effect was achieved for lower concentrations of the inhibitors (in the presence of malaoxon/isomalathion at concentrations 2 £ 10 27 M/2 £ 10 27 M, 2 £ 10 27 M/3 £ 10 27 M and 2 £ 10 27 M/4.5 £ 10 27 M), while an antagonistic effect was obtained for all higher concentrations of inhibitors. The presence of a non-inhibitory degradation product (phosphorodithioic O,O,S-trimethyl ester) did not affect the inhibition efficiencies of the malathion by-products, malaoxon and isomalathion.

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Section: Inhibition (%) [Concentration Of Inhibitor (M)]mentioning

confidence: 99%

Inhibition of AChE by malathion and some structurally similar compounds

Krstić

Čolović

Kralj

et al. 2008

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…The modified GCE was used as a working electrode. Area of working electrode used is 0.0707 cm 2 . Platinum wire and Ag/ AgCl were employed as an auxiliary and reference electrode respectively.…”

Section: Apparatusmentioning

confidence: 99%

Voltammetric Determination of Phenoxybenzyl‐Type Insecticides at Chemically Modified Conducting Polymer‐Carbon Nanotubes Coated Electrodes

et al. 2008

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The electrochemical reduction of three common insecticides such as cypermethrin (CYP), deltamethrin (DEL) and fenvalerate (FEN) was investigated at glassy carbon electrode (GCE), multiwalled carbon nanotubes modified GCE (MWCNT-GCE), polyaniline (herein called as modifier M 1 ) and polypyrrole (herein called as modifier M 2 ) deposited MWCNT/GCE using cyclic voltammetry. Influences of pH, scan rate, and concentration were studied. The surface morphology of the modified film was characterized by scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD). A systematic study of the experimental parameters that affect differential pulse stripping voltammetry (DPSV) was carried out and the optimized experimental conditions were arrived at. The calibration plots were linear over the insecticides concentration range 0.1 -100 mg L À1 and 0.05 -100 mg L À1 for all the three insecticides at MWCNT-GCE and MWCNT(M 1 )-GCE respectively. The MWCNT(M 2 )-GCE performed well among the three electrode systems and the determination range obtained was 0.01 -100 mg L À1 for CYP, DEL and FEN. The limit of detection (LOD) was 0.35 mg L À1, 0.9 mg L À1 and 0.1 mg L À1 for CYP, DEL and FEN respectively on MWCNT(M 2 )-GCE modified system. Suitability of this method for the trace determination of insecticide in spiked soil sample was also determined.

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Section: Conclusion and Prospectsmentioning

confidence: 99%

“…Including well-designed electrophysiological and behavioural bioassays can provide essential additional information about bioactive compounds that cannot or can barely be detected in chemical analyses. In future studies biosensing techniques including electronic noses [203][204][205][206] might add to classical chemical analyses of HIPVs. If biologists, chemists and engineers join forces in this effort they will undoubtedly be able to fully unravel the fascinating world of chemically-mediated interactions between plants and their biotic environment.…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Advances and challenges in the identification of volatiles that mediate interactions among plants and arthropods

D’Alessandro

Turlings

2006

Analyst

148

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The relatively new research field of Chemical Ecology has, over the last two decades, revealed an important role of plant-produced volatile organic compounds (VOCs) in mediating interactions between plants and other organisms. Of particular interest are the volatile blends that plants actively emit in response to herbivore damage. Various efforts are underway to pinpoint the bioactive compounds in these complex blends, but this has proven to be exceedingly difficult. Here we give a short overview on the role of herbivore-induced plant volatiles in interactions between plants and other organisms and we review methods that are currently employed to collect and identify key volatile compounds mediating these interactions. Our perspective on future directions of this fascinating research field places special emphasis on the need for an interdisciplinary approach. Joint efforts by chemists and biologists should not only facilitate the elucidation of crucial compounds, but can also be expected to lead to an exploitation of this knowledge, whereby ecological interactions may be chemically manipulated in order to protect crops and the environment.

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Biosensing in high-performance chemical separations

Cited by 20 publications

References 131 publications

Inhibition of AChE by malathion and some structurally similar compounds

Inhibition of AChE by malathion and some structurally similar compounds

Voltammetric Determination of Phenoxybenzyl‐Type Insecticides at Chemically Modified Conducting Polymer‐Carbon Nanotubes Coated Electrodes

Advances and challenges in the identification of volatiles that mediate interactions among plants and arthropods

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