Biosensing enhancement of dengue virus using microballoon mixers on centrifugal microfluidic platforms

Aeinehvand, Mohammad Mahdi; Ibrahim, Fatimah; Harun, Sulaiman Wadi; Djordjević, Ivan; Hosseini, Samira; Rothan, Hussin A.; Yusof, Rohana; Madou, Marc

doi:10.1016/j.bios.2014.08.076

Cited by 37 publications

(26 citation statements)

References 34 publications

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“…Aeinehvand et al utilized microballoons inside a centrifugal microfluidic device to enhance mixing [91]. This enhancement was obtained through oscillatory expansion and contraction of the balloon in order to alter the fluid flow.…”

Section: Enhanced Mixing By Active Forcesmentioning

confidence: 99%

Mixing in microfluidic devices and enhancement methods

Ward

Fan

2015

J. Micromech. Microeng.

337

229

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Mixing in microfluidic devices presents a challenge due to laminar flows in microchannels, which result from low Reynolds numbers determined by the channel’s hydraulic diameter, flow velocity, and solution’s kinetic viscosity. To address this challenge, novel methods of mixing enhancement within microfluidic devices have been explored for a variety of applications. Passive mixing methods have been created, including those using ridges or slanted wells within the microchannels, as well as their variations with improved performance by varying geometry and patterns, by changing the properties of channel surfaces, and by optimization via simulations. In addition, active mixing methods including microstirrers, acoustic mixers, and flow pulsation have been investigated and integrated into microfluidic devices to enhance mixing in a more controllable manner. In general, passive mixers are easy to integrate, but difficult to control externally by users after fabrication. Active mixers usually take efforts to integrate within a device and they require external components (e.g. power sources) to operate. However, they can be controlled by users to a certain degree for tuned mixing. In this article, we provide a general overview of a number of passive and active mixers, discuss their advantages and disadvantages, and make suggestions on choosing a mixing method for a specific need as well as advocate possible integration of key elements of passive and active mixers to harness the advantages of both types.

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Section: Enhanced Mixing By Active Forcesmentioning

confidence: 99%

Mixing in microfluidic devices and enhancement methods

Ward

Fan

2015

J. Micromech. Microeng.

337

229

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show abstract

“…The integration of microfluidic and LOCs reduces detection time and cost [111,112]. The use of microfluidics to detect dengue virus in cell lysates and serum samples has been reported by several authors with a promising limit of detection [113,114].…”

Section: Microfluidic and Lab-on-a-chip Platformsmentioning

confidence: 99%

Challenges and Opportunities of Laboratory diagnosis of Dengue virus infection: a review

Mwanyika

Misinzo

Rugarabamu

et al. 2019

Preprint

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Background: Globally, dengue is one of the most important mosquito-borne viral diseases. Lack of effective vaccines and specific therapy against the disease threaten global health. Reliance on clinical diagnosis is complex due to clinical manifestations which resemble other diseases. This review examined various challenges of current dengue laboratory diagnoses, emerging technological opportunities and highlights considerations for future dengue diagnoses. Methods: A literature search from PubMed, Web of Science and Google Scholar databases was done from October 2018 to January 2019. Thematic descriptive analysis was done for all qualitative data and quantitative data analysis for computation of sensitivity and specificity of selected diagnostic tests at 95% confidence was done using R software (v3.4.4, mada package). The results: A total of 128 articles was reviewed. The current dengue laboratory diagnoses include (i) virus isolation (ii) detection of nucleic acid (iii) detection of non-structural protein 1(NS1) antigen and (iv) detection of anti-dengue antibodies. Assessment of diagnostic performance shows that reverse transcription-polymerase chain reaction (RT-PCR) and IgM antibody capture enzyme-linked immunosorbent assay (IgM ELISA) have high and consistent sensitivity (82.6% to 99.2% and 92.8% to 97.8%, respectively) and specificity (78.8% to 100% and 80.3% to 99%, respectively) compared to NS1 ELISA and NS1 commercial rapid tests with sensitivity (53.7% to 96.2% and 49.7% to 99.5%, respectively) and specificity (34.5% to 93.8% and 63.8% to 98.6%, respectively). Major challenges of dengue laboratory diagnosis include lack of reliable tests for routine purposes. Routine dengue tests are mainly serological, which are not suitable for discriminating dengue virus from other infecting flaviviruses due to cross-reactivity, narrow window of diagnosis due to short virus life cycle and inconsistent performance of commercial rapid tests. New technologies such as biosensors and nanobodies are being developed to improve sensitivity, specificity, detection time and reduce the cost. However, the performance of these new tools under field condition is unknown. Conclusion: Currently, RT-PCR and IgM ELISA are the most sensitive and specific dengue diagnostic tests, despite their limitations. Future research should explore emerging technologies to improve the sensitivity and specificity of dengue diagnostics.

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“…Similarly, Gunda and colleagues made use of dengue virus NS1 bound to micropillars to improve the surface area for binding and subsequently, enhance detection sensitivity (Gunda et al, 2013). Aeinehvand and team incorporated a microballoon mixer (that expands and contracts) with centrifugal microfluidic platform to improve the sensitivity of ELISA detection of dengue virus due to improved mixing (Aeinehvand et al, 2015). Recently, Zhang and colleagues developed a multi-stack paper immunoassay that removes proteinaceous substance in the saliva before detection of target antigen with adsorbed antibody (Zhang et al, 2015).…”

Section: Other Infectious Diseases (Sars Dengue Tuberculosis)mentioning

confidence: 99%

Advances in microfluidics in combating infectious diseases

Tay

Pavesi

Yazdi

et al. 2016

Biotechnology Advances

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One of the important pursuits in science and engineering research today is to develop low-cost and user-friendly technologies to improve the health of people. Over the past decade, research efforts in microfluidics have been made to develop methods that can facilitate low-cost diagnosis of infectious diseases, especially in resource-poor settings. Here, we provide an overview of the recent advances in microfluidic devices for point-of-care (POC) diagnostics for infectious diseases and emphasis is placed on malaria, sepsis and AIDS/HIV. Other infectious diseases such as SARS, tuberculosis, and dengue are also briefly discussed. These infectious diseases are chosen as they contribute the most to disability-adjusted life-years (DALYs) lost according to the World Health Organization (WHO). The current state of research in this area is evaluated and projection toward future applications and accompanying challenges are also discussed.

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Biosensing enhancement of dengue virus using microballoon mixers on centrifugal microfluidic platforms

Cited by 37 publications

References 34 publications

Mixing in microfluidic devices and enhancement methods

Mixing in microfluidic devices and enhancement methods

Challenges and Opportunities of Laboratory diagnosis of Dengue virus infection: a review

Advances in microfluidics in combating infectious diseases

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