Biosensing based on surface plasmon resonance and living cells

Chabot, Vincent; Cuerrier, Charles M.; Escher, Emanuel; Aimez, Vincent; Grandbois, Michel; Charette, Paul G.

doi:10.1016/j.bios.2008.08.025

Cited by 126 publications

(80 citation statements)

References 40 publications

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“…At this concentration, the SPR signal was found to stabilize after some time, a feature that could be attributed to the reversible character of this cell perturbation for a portion of the cell population. It should also be mentioned that the SPR signature for TRAILinduced apoptosis is distinct from the one produced when G-protein coupled receptors such as the angiotensin and thrombin receptors are stimulated [24,25]. Altogether, these results suggest that cell rounding, detachment from substrate or loss of cellular membrane integrity can be easily monitored by SPR and that these events contribute to the SPR signature associated with cell apoptotic events.…”

Section: Spr Monitoring Of Apoptosismentioning

confidence: 87%

“…However, proof of concept has been made that SPR biosensing can also be used in combination with living cells to monitor the effects of different molecular stimuli on cellular activities [18][19][20][21]. Notably, SPR has been used to study the ligand-induced reaction of mast cells [22], the activation of olfactory receptors by odorant molecules [23] and the stimulation of various cell receptors [24][25][26].…”

Section: Introductionmentioning

confidence: 98%

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Label-free monitoring of apoptosis by surface plasmon resonance detection of morphological changes

et al. 2012

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Apoptosis can be routinely characterized using biomolecular markers such as in the TUNEL and the annexin V assays or by using fluorescent caspase substrates. Apoptosis can also be semi-quantitatively characterized using microscopy, which targets morphological features such as cell rounding, nuclear condensation and fragmentation as well as cell membrane blebbing. This label-free approach provides a limited resolution for the evolution of these events in time and relies heavily on subjective identification of the morphological features. Here we propose a label-free assay based on surface plasmon resonance (SPR) detection of minute morphology changes occurring as a result of apoptosis induction in an endothelial cell model (EA.hy926). At first, annexin V assays confirmed that our cellular model was responsive to TRAIL over a 12-hour period. Then, we show that SPR allows accurate monitoring of apoptosis by measuring (1) the duration of the latency period during which the apoptotic signal is integrated by the initiator caspases and transmitted to the executioner caspases, (2) the rate of the execution phase in which death substrates are cleaved and morphological changes occur, and (3) the total extent of apoptosis. Using these parameters, we characterized the responses obtained with TRAIL (EA.hy926, HeLa, AD-293) and the anti-Fas antibody (HeLa) for the extrinsic pathways and UV exposure (HeLa) for the intrinsic pathways. By comparing the SPR time-course of apoptosis with phase contrast micrographs, we demonstrate that the cell morphological hallmarks of apoptosis are the major contributors to the SPR signal. Altogether, our results validate the use of SPR as an accurate label-free assay for the real-time monitoring of apoptosis-triggered cell morphological changes.

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Section: Spr Monitoring Of Apoptosismentioning

confidence: 87%

Section: Introductionmentioning

confidence: 98%

Label-free monitoring of apoptosis by surface plasmon resonance detection of morphological changes

et al. 2012

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“…This can provide useful information about of the stoichiometry of the analyzed molecular interaction [15]. Besides the kinetic and thermodynamic interpretation of the SPR signals, for whole-cell sensors, the observation of the change in refractive index at the sensor surface can also be used as an indicator for morphological changes in the basal portions of living cells adhering to these surfaces [16].…”

Section: Theoretical Background Of Spr Biosensorsmentioning

confidence: 99%

Surface plasmon resonance sensors in cell biology: basics and application

Robelek

2009

Bioanal Rev

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Optical sensors based on the excitation of surface plasmons, referred to as surface plasmon resonance (SPR) sensors, have become a central analytical tool for characterizing and quantifying a wide variety of macromolecular interactions, like receptor-ligand contacts. Besides this classical field of application, in the last 15 years, the development of SPR sensors aiming for the detection and analysis of ligand/cell or host/pathogen interactions, cell/ cell contacts, and cellular reactions gained considerable momentum. The number of publications reporting about applications of SPR sensors implementing vital prokaryotic or eukaryotic cells as biorecognition elements for medical diagnostics, environmental monitoring, or biological safety is steadily growing. This review gives a short introduction to the technique of surface plasmon resonance and the parameters that are important for its application in the field of vital cell sensors. Furthermore, the publications concerning the application of such sensors in the analysis of cellular interactions and cellular reactions to extra-and intracellular stimuli are summarized.

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“…[19][20][21] The response of cells to external stimuli such as drug and protein therapeutics can also be monitored in real-time with SPR and related optical sensing technologies such as optical waveguide, thereby providing a powerful screening technology. [22][23][24] SPR sensing of cells is, however, intrinsically complex, as they are inherently heterogeneous from an optical standpoint. In addition, the characteristic dimensions of plasmonic evanescent waves are of similar magnitude (in regard to propagation) or significantly smaller (in regard to penetration) than cellular dimensions.…”

Section: Introductionmentioning

confidence: 99%

Cellular Micromotion Monitored by Long-Range Surface Plasmon Resonance with Optical Fluctuation Analysis

et al. 2015

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Long-range surface plasmon resonance (LRSPR) is a powerful biosensing technology due to a substantially larger probing depth into the medium and sensitivity, compared with conventional SPR. We demonstrate here that LRSPR can provide sensitive noninvasive measurement of the dynamic fluctuation of adherent cells, often referred to as the cellular micromotion. Proof of concept was achieved using confluent layers of 3T3 fibroblast cells and MDA-MB-231 cancer cells. The slope of the power spectral density (PSD) of the optical fluctuations was calculated to determine the micromotion index, and significant differences were measured between live and fixed cell layers. Furthermore, the performances of LRSPR and conventional surface plasmon resonance (cSPR) were compared with respect to micromotion monitoring. Our study showed that the micromotion index of cells measured by LRSPR sensors was higher than when measured with cSPR, suggesting a higher sensitivity of LRSPR to the micromotion of cells. To investigate further this finding, simulations were conducted to establish the relative sensitivities of LRSPR and cSPR to membrane fluctuations. Increased signal intensity was predicted for LRSPR in comparison to cSPR, suggesting that membrane fluctuations play a significant role in the optical micromotion measured in LRSPR. Analogous to cellular micromotion measured using impedance techniques, LRSPR micromotion has the potential to provide important biological information on the metabolic activity and viability of adherent cells.

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Biosensing based on surface plasmon resonance and living cells

Cited by 126 publications

References 40 publications

Label-free monitoring of apoptosis by surface plasmon resonance detection of morphological changes

Label-free monitoring of apoptosis by surface plasmon resonance detection of morphological changes

Surface plasmon resonance sensors in cell biology: basics and application

Cellular Micromotion Monitored by Long-Range Surface Plasmon Resonance with Optical Fluctuation Analysis

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