Pectinase is a collection of enzymes that degrade pectin into simpler compounds known as galacturonic acids. Microbes and plants largely produce pectinase, and it has a wide range of biotechnological applications in food, agriculture, industry, the environment and medicine. Fruit juices, coffee, cocoa, tea, jams and jellies are all made with pectinase in the food industry. Furthermore, pectinase, a supplemental enzyme found in animal feed, increases nutrient absorption. Pectinase has been utilized in the agricultural sector for plant virus purification, oil extraction, retting and degumming processes, and cotton fiber bio-scouring. They play a critical function in wastewater treatment by facilitating pectin breakdown in the environmental sector. Pectinases are mostly used in the wine and paper industries in the industrial sector. Pectinases are probiotic enzymes that are introduced to a variety of foods in medicine. This review aims to compile all of the accessible information on pectinase enzymes' biotechnological applications in diverse industries.