Biorheological changes of dendritic cells at the different differentiation stages

Wang, Xianwei; Chen, Dan; Zhang, Xiaolan; Jia, Binbin; Xie, Lide; Sun, Dawei; Ka, Weibo; Yao, Weijuan; Wen, Zhiyuan

doi:10.3233/ch-2010-1334

Cited by 4 publications

(2 citation statements)

References 25 publications

(32 reference statements)

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“…EPM is one of the biophysical parameters, which reflect the negative charges on the surface of cell membrane. Our previous studies have shown that DCs’ EPM continuously increased during their differentiation process [ 42 ]. Here, we found that EPMs of mDCs were decreased after the treatment of IL-10 ( Fig 1 ), suggesting that the surface charges on the membrane of mDCs were reduced.…”

Section: Discussionmentioning

confidence: 99%

Interleukin-10 reorganizes the cytoskeleton of mature dendritic cells leading to their impaired biophysical properties and motilities

Liu

Long

et al. 2017

PLoS ONE

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Interlukin-10 (IL-10) is an immunomodulatory cytokine which predominantly induces immune-tolerance. It has been also identified as a major cytokine in the tumor microenvironment that markedly mediates tumor immune escape. Previous studies on the roles of IL-10 in tumor immunosuppression mainly focus on its biochemical effects. But the effects of IL-10 on the biophysical characteristics of immune cells are ill-defined. Dendritic cells (DCs) are the most potent antigen-presenting cells and play a key role in the anti-tumor immune response. IL-10 can affect the immune regulatory functions of DCs in various ways. In this study, we aim to explore the effects of IL-10 on the biophysical functions of mature DCs (mDCs). mDCs were treated with different concentrations of IL-10 and their biophysical characteristics were identified. The results showed that the biophysical properties of mDCs, including electrophoresis mobility, osmotic fragility and deformability, as well as their motilities, were impaired by IL-10. Meanwhile, the cytoskeleton (F-actin) of mDCs was reorganized by IL-10. IL-10 caused the alternations in the expressions of fasin1 and profilin1 as well as the phosphorylation of cofilin1 in a concentration-dependent fashion. Moreover, Fourier transformed infrared resonance data showed that IL-10 made the status of gene transcription and metabolic turnover of mDCs more active. These results demonstrate a new aspect of IL-10’s actions on the immune system and represent one of the mechanisms for immune escape of tumors. It may provide a valuable clue to optimize and improve the efficiency of DC-based immunotherapy against cancer.

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Section: Discussionmentioning

confidence: 99%

Interleukin-10 reorganizes the cytoskeleton of mature dendritic cells leading to their impaired biophysical properties and motilities

Liu

Long

et al. 2017

PLoS ONE

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“…The electrophoretic mobility of DCs was associated with the presence of negative charges on their surface membrane. It has be established that the number of these charges continuously increases during the differentiation process (maturation and activation) of the DCs, which is directly linked with their movability [22]. Decrease of these charges on the surface of the cell membrane leads to an increase in the adhesion forces of the DCs to the components of the tumor microenvironment, and limits the motility of the DCs.…”

Section: Discussionmentioning

confidence: 99%

Study of Dendritic Cell Migration Using Cell-Iq Analysis System

Nekhaeva¹,

Данилова²,

Балдуева³

2018

Sib. onkol. ž.

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Dendritic cells (DCs) belong to specialized pool of antigen-presenting cells with high functional plasticity and manifest with immunostimulatory or immunosuppressive potential depending on sequence and combination of microenvironment stimuli, which determine their differentiation, maturation and activation. The use of antitumor DCs vaccines is based on the ability of DCs specifically activated in vitro migrate for antigen presentation to T- lymphocytes. We studied the components of the tumor microenvironment that are capable of inhibiting DCs migration. The study of the mobility of DCs in Cell-IQ experimental analytical system showed the presence of an inverse correlation of high strength between the average trajectory speed and the level of immunosuppressive factors (ISFs) in supernatants of cultured skin melanoma cells (TGFβ1, IL-10, IL-18, VEGF-A, EGF, FGF, HGF, sFASL (p<0.01). An inverse relation of high force was found between the movement angle of the DCs population and the expression of cancer testis antigens (CTAs) and other tumor- associated antigens (TAAs) on tumor cells (Melan A, tyrosinase, families of MAGE, BAGE, NY- ESO-1 (p<0.01)). The speed of DCs movement in culture system with melanoma cells #894 was 30.10±2.23 μm/h and differed from that in the presence of IL-10 1 ng/ml (10.45±0.52 μm/h), TGFβ1 10 ng/ml (14.32±0.42 μm/h), VEGF 50 ng/ml (18.7±1.89 μm/h) (p<0.05). One can assume that content of this ISFs in the blood is one of the factors determining clinical efficacy of DCs immune therapy.

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The influence of polystyrene and poly(ether imide) inserts with different roughness, on the activation of dendritic cells

Roch

Kratz

et al. 2013

Clinical Hemorheology and Microcirculation

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Dendritic cells (DC) have a pivotal role during inflammation. DC efficiently present antigens to T cells and shape the subsequent immune response by the secretion of pro-or anti-inflammatory cytokines and by the expression of co-stimulatory molecules. They respond to "danger signals" such as microbial products or fragments from necrotic cells or tissues, but were also described to be reactive towards biomaterials. However, how mechanical and physical properties of the subjacent substrate influences the DC activation is currently poorly understood. In this study micro patterned inserts prepared from polystyrene (PS) as well as from poly (ether imide) (PEI) with three different roughness levels of i) R q = 0.29 m (PS) and 0.23 m (PEI); ii) R q = 3.47 m (PS) and 3.92 m (PEI); and iii) R q = 22.16 m (PS) and 22.65 m (PEI) were analyzed for their capacity to influence the activation of human monocytes derived DC. Since the DC were directly cultured in the inserts, the effects of the testing material alone could be investigated and influences from additional culture dish material could be excluded.The viability, the expression of the DC activation markers, and their cytokine/chemokine secretion were determined after the incubation with the different inserts in vitro.Both the PS and the PEI inserts did not influence the survival of the DC and their expression of co-stimulatory molecules. The expression of inflammatory cytokines was not altered by the PEI and PS inserts. However, the secretion of chemokines such as CCL2, CCL3, and CCL4 was influenced by the different roughness levels, indicating that material roughness has the capacity to modulate the DC phenotype.The data presented here will help to understand the interaction of DC with structured polymer surfaces. Biomaterial-induced immuno-modulatory effects mediated by DC may promote tissue regeneration or could potentially reduce inflammation caused by the implant material.

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Biorheological changes of dendritic cells at the different differentiation stages

Cited by 4 publications

References 25 publications

Interleukin-10 reorganizes the cytoskeleton of mature dendritic cells leading to their impaired biophysical properties and motilities

Interleukin-10 reorganizes the cytoskeleton of mature dendritic cells leading to their impaired biophysical properties and motilities

Study of Dendritic Cell Migration Using Cell-Iq Analysis System

The influence of polystyrene and poly(ether imide) inserts with different roughness, on the activation of dendritic cells

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