Bioreporters and biosensors for arsenic detection. Biotechnological solutions for a world-wide pollution problem

Merulla, Davide; Buffi, Nina; Beggah, Siham; Truffer, Frédéric; Geiser, Martial; Renaud, Philippe; Meer, Jan Roelof van der

doi:10.1016/j.copbio.2012.09.002

Cited by 64 publications

(43 citation statements)

References 61 publications

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“…The construction of a significant number of whole-cell arsenic biosensors has been reported in the literature (7,30), which hence raises the question of why further sensors were constructed. However, most of the biosensors use E. coli as a host, whereas no biosensors using P. putida strains have been reported.…”

Section: Acidithiobacillus Ferrooxidansmentioning

confidence: 99%

“…We report here the generation of purified, recombinantly produced ArsR1 and ArsR2 and their analysis by an array of biochemical and biophysical approaches. As mentioned above, environmental arsenic monitoring is of central importance, and several ArsR-based biosensors have been described (7,30). In the second part of this work, we describe the construction of ArsR1-and ArsR2-based biosensors.…”

mentioning

confidence: 99%

“…In fact, it was placed first on the priority list of hazardous compounds issued by the U.S. Agency for Toxic Substances and Disease Registry (see http://www .atsdr.cdc.gov/SPL/index.html). Of particular relevance is groundwater contamination with arsenic in Asiatic countries (5,6), and environmental arsenic monitoring is thus of primary importance (7,8).…”

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confidence: 99%

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Paralogous Regulators ArsR1 and ArsR2 of Pseudomonas putida KT2440 as a Basis for Arsenic Biosensor Development

Fernández

Morel

Ramos

et al. 2016

Appl Environ Microbiol

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The remarkable metal resistance of many microorganisms is related to the presence of multiple metal resistance operons. Pseudomonas putida KT2440 can be considered a model for these microorganisms since its arsenic resistance is due to the action of proteins encoded by the two paralogous arsenic resistance operons ARS1 and ARS2. Both operons contain the genes encoding the transcriptional regulators ArsR1 and ArsR2 that control operon expression. We show here that purified ArsR1 and ArsR2 bind the trivalent salt of arsenic (arsenite) with similar affinities (~30 M), whereas no binding is observed for the pentavalent salt (arsenate). Furthermore, trivalent salts of bismuth and antimony showed binding to both paralogues. The positions of cysteines, found to bind arsenic in other homologues, indicate that ArsR1 and ArsR2 employ different modes of arsenite recognition. Both paralogues are dimeric and possess significant thermal stability. Both proteins were used to construct whole-cell, lacZ-based biosensors. Whereas responses to bismuth were negligible, significant responses were observed for arsenite, arsenate, and antimony. Biosensors based on the P. putida arsB1 arsB2 arsenic efflux pump double mutant were significantly more sensitive than biosensors based on the wild-type strain. This sensitivity enhancement by pump mutation may be a convenient strategy for the construction of other biosensors. A frequent limitation found for other arsenic biosensors was their elevated background signal and interference by inorganic phosphate. The constructed biosensors show no interference by inorganic phosphate, are characterized by a very low background signal, and were found to be suitable to analyze environmental samples. IMPORTANCE Arsenic is at the top of the priority list of hazardous compounds issued by the U.S. Agency for Toxic Substances and Disease. The reason for the stunning arsenic resistance of many microorganisms is the existence of paralogous arsenic resistance operons.Pseudomonas putida KT2440 is a model organism for such bacteria, and their duplicated ars operons and in particular their ArsR transcription regulators have been studied in depth by in vivo approaches. Here we present an analysis of both purified ArsR paralogues by different biophysical techniques, and data obtained provide valuable insight into their structure and function. Particularly insightful was the comparison of ArsR effector profiles determined by in vitro and in vivo experimentation. We also report the use of both paralogues to construct robust and highly sensitive arsenic biosensors. Our finding that the deletion of both arsenic efflux pumps significantly increases biosensor sensitivity is of general relevance in the biosensor field.A rsenic is omnipresent in nature and found in many environmental biotopes such as seawater, freshwater, soils, and rocks. It is either released through various natural processes such as weathering or hydrothermal emissions or generated by a number of anthropogenic activities such as the use of arse...

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Section: Acidithiobacillus Ferrooxidansmentioning

confidence: 99%

mentioning

confidence: 99%

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Paralogous Regulators ArsR1 and ArsR2 of Pseudomonas putida KT2440 as a Basis for Arsenic Biosensor Development

Fernández

Morel

Ramos

et al. 2016

Appl Environ Microbiol

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“…10,11 Developing cost-effective biosensors might provide a complementary solution. 12 For example, genetically engineered bacterial cells, 13 and enzyme inhibition assays were developed to detect arsenic. 14,15 On the chemical sensor side, a few optical and electrochemistry assays were reported.…”

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confidence: 99%

DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection

2014

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“…For example, As-bioreporters exist which produce fluorescence, bioluminescence, visible colors, current or pH changes. 10 Comparative field assays with extremely simple bacterial bioreporter technology demonstrated excellent quantification and low falsenegative sample measurements. 11,12 Since bioreporter assays still require a certain degree of manipulation of the cells, we sought to design and produce a standalone device in which cells would be embedded using microfluidic cartridges, and that would further automatically sample, read-out and record the reporter cell's response.…”

Section: Introductionmentioning

confidence: 99%

Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells

Truffer

Buffi

Merulla

et al. 2014

Review of Scientific Instruments

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We present a compact portable biosensor to measure arsenic As(III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentration (between 0 and 100 μg/L). The device accommodates a small polydimethylsiloxane microfluidic chip that holds the agarose-encapsulated bacteria, and a complete optical illumination/collection/detection system for automated quantitative fluorescence measurements. The device is capable of sampling water autonomously, controlling the whole measurement, storing and transmitting data over GSM networks. We demonstrate highly reproducible measurements of arsenic in drinking water at 10 and 50 μg/L within 100 and 80 min, respectively.

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Bioreporters and biosensors for arsenic detection. Biotechnological solutions for a world-wide pollution problem

Cited by 64 publications

References 61 publications

Paralogous Regulators ArsR1 and ArsR2 of Pseudomonas putida KT2440 as a Basis for Arsenic Biosensor Development

Paralogous Regulators ArsR1 and ArsR2 of Pseudomonas putida KT2440 as a Basis for Arsenic Biosensor Development

DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection

Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells

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