Bioremediation aspects of crude oil-polluted fields can be achieved by isolating and identifying bacterial species from oil-contaminated soil. This allows for the selection of the most active isolates and the enhancement of the effectiveness of other bacteria. This project will be a base to use green technology for clean the oil contaminated soil in Jordan. This study involved the isolation and identification of oil-degrading microbes from soil samples contaminated with oil in the northeastern region of Jordan. The morphological and biochemical tests were used to characterize twenty-five bacterial isolates. Molecular identification of a universal primer 16S rDNA gene was used to identify bacterial isolates. Total petroleum hydrocarbons were analyzed using gas chromatography for soil samples. All soil samples were analyzed for heavy metal contamination (Cu, Cd, Mn, Zn, and Pb). The bacterial growth count (CFU/g) was between 1.06×10 5 and 2.80×10 17 . The identified bacterial genera included: Staphylo-