Bioremediation on the Shore after an Oil Spill from the Nakhodka in the Sea of Japan. III. Field Tests of a Bioremediation Agent with Microbiological Cultures for the Treatment of an Oil Spill

Trials of bioremediation on a beach affected by the heavy oil spill of the Prestige

“…The digital photographs were converted to monochrome images with 256 gradations of grey (level of darkness between 0 and 255) with image processing software (Adobe ® Photoshop ® CS2, version 9.0). Then, the pixels were classified into two groups according to the darkness of they grey colours (black pixels in group 1 and white pixels in group 2) [8]. The black pixels represented the areas which were coated by the residual oil whereas the white pixels indicated those areas which were residual oil free.…”

Autochthonous bioaugmentation and its possible application to oil spills

“…It was shown that TerraZyme was able to enhance the biodegradation of oil on the shore (Tsutsumi et al 2000). However, such exogenous microbial products have never been widely used in Japan.…”

Evaluation of biodiesel as bioremediation agent for the treatment of the shore affected by the heavy oil spill of the Prestige

“…Several shoreline studies have been carried out to assess the effectiveness of bioremediation on oil spills in the field, both after a real spill incident and intentionally releasing oil into the environment [2][3][4][5]. Nevertheless, bioremediation amendments were usually tested on sediments whereas few trials were performed with oil polluted rocky substrates [6,7], which were the target of our study since rocks were seriously affected by the Prestige oil spill. Since the water-soluble amendments are easily drained away when applied to a rocky surface, oleophilic fertilizers could be an alternative because they are able to adhere to oil and provide the oil-water interface, where biodegradation mainly occurs [8], with nutrients.…”