Bioremediation of the nematicide oxamyl by Enterobacter ludwigii isolated from agricultural wastewater

Radwan, Tharwat; Reyad, Amany M; Essa, Ashraf M. M.

doi:10.5455/egyjebb.20170131064321

Cited by 4 publications

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“…(ENSG301 and ENSG305), A. lwoffii (ENSG302), K. pneumoniae (ENSG303), and B. thuringiensis (ENSW401) were identified ( Table 1 ). Except uncultured Vitreoscilla sp., all these bacteria have been isolated from different wastewaters ( Zabłocka-Godlewska et al, 2012 ; Singh et al, 2015 ; Khan et al, 2015 ; Zhi et al, 2016 ; Radwan et al, 2017 ; Maintinguer et al, 2017 ). Importantly, the ability of these bacteria to produce the biofilms in the glass test tubes with SOBG broth was not examined yet.…”

Section: Discussionmentioning

confidence: 99%

Metal-Adapted Bacteria Isolated From Wastewaters Produce Biofilms by Expressing Proteinaceous Curli Fimbriae and Cellulose Nanofibers

et al. 2018

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Bacterial biofilm plays a pivotal role in bioremediation of heavy metals from wastewaters. In this study, we isolated and identified different biofilm producing bacteria from wastewaters. We also characterized the biofilm matrix [i.e., extracellular polymeric substances (EPS)] produced by different bacteria. Out of 40 isolates from different wastewaters, only 11 (27.5%) isolates (static condition at 28°C) and 9 (22.5%) isolates (agitate and static conditions at 28 and 37°C) produced air–liquid (AL) and solid–air–liquid (SAL) biofilms, respectively, only on salt-optimized broth plus 2% glycerol (SOBG) but not in other media tested. Biomass biofilms and bacteria coupled with AL biofilms were significantly (P ≤ 0.001) varied in these isolates. Escherichia coli (isolate ENSD101 and ENST501), Enterobacter asburiae (ENSD102), Enterobacter ludwigii (ENSH201), Pseudomonas fluorescens (ENSH202 and ENSG304), uncultured Vitreoscilla sp. (ENSG301 and ENSG305), Acinetobacter lwoffii (ENSG302), Klebsiella pneumoniae (ENSG303), and Bacillus thuringiensis (ENSW401) were identified based on 16S rRNA gene sequencing. Scanning electron microscope (SEM) images revealed that biofilm matrix produced by E. asburiae ENSD102, uncultured Vitreoscilla sp. ENSG301, A. lwoffii ENSG302, and K. pneumoniae ENSG303 are highly fibrous, compact, and nicely interlinked as compared to the biofilm developed by E. ludwigii ENSH201 and B. thuringiensis ENSW401. X-ray diffraction (XRD) results indicated that biofilm matrix produced by E. asburiae ENSD102, uncultured Vitreoscilla sp. ENSG301, and A. lwoffii ENSG302 are non-crystalline amorphous nature. Fourier transform infrared (FTIR) spectroscopy showed that proteins and polysaccharides are the main components of the biofilms. Congo red binding results suggested that all these bacteria produced proteinaceous curli fimbriae and cellulose-rich polysaccharide. Production of cellulose was also confirmed by Calcofluor binding- and spectrophotometric assays. E. asburiae ENSD102, Vitreoscilla sp. ENSG301, and A. lwoffii ENSG302 were tested for their abilities to form the biofilms exposure to 0 to 2000 mg/L of copper sulfate (for Cu), zinc sulfate (for Zn), lead nitrate (for Pb), nickel chloride (for Ni), and potassium dichromate (for Cr), several concentrations of these metals activated the biofilm formation. The polysaccharides is known to sequester the heavy metals thus, these bacteria might be applied to remove the heavy metals from wastewater.

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Section: Discussionmentioning

confidence: 99%

Metal-Adapted Bacteria Isolated From Wastewaters Produce Biofilms by Expressing Proteinaceous Curli Fimbriae and Cellulose Nanofibers

et al. 2018

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“…Jetiyanon, 2015;Kang et al, 2015;Paul and Mukherjee, 2016;Ren et al, 2019;Mahdi et al, 2020;Haque et al, 2021a E. ludwigii Degradation of dyes, biosorption of heavy metals, functions as nematicides, solubilization of nutrients, reduction of cadmium stress in plants, plant growth enhancer, and prevention of drought and salinity stress in crop plants. Shoebitz et al, 2009;Gontia−Mishra et al, 2016;Radwan et al, 2017;Adhikari et al, 2020;Haque et al, 2021b Vitreoscilla sp. Decolorization, degradation, and detoxification of textile dyes; hemoglobin technology used in bio-product synthesis; bioremediation; and enhancement of tolerance of submergence, oxidative, and nitrosative stress in plants.…”

Section: Bacteriamentioning

confidence: 99%

Biofilm Formation, Production of Matrix Compounds and Biosorption of Copper, Nickel and Lead by Different Bacterial Strains

et al. 2021

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Bacterial biofilms play a key role in metal biosorption from wastewater. Recently, Enterobacter asburiae ENSD102, Enterobacter ludwigii ENSH201, Vitreoscilla sp. ENSG301, Acinetobacter lwoffii ENSG302, and Bacillus thuringiensis ENSW401 were shown to form air–liquid (AL) and solid–air–liquid (SAL) biofilms in a static condition at 28 and 37°C, respectively. However, how environmental and nutritional conditions affect biofilm formation; production of curli and cellulose; and biosorption of copper (Cu), nickel (Ni), and lead (Pb) by these bacteria have not been studied yet. In this study, E. asburiae ENSD102, E. ludwigii ENSH201, and B. thuringiensis ENSW401 developed the SAL biofilms at pH 8, while E. asburiae ENSD102 and Vitreoscilla sp. ENSG301 constructed the SAL biofilms at pH 4. However, all these strains produced AL biofilms at pH 7. In high osmolarity and ½-strength media, all these bacteria built fragile AL biofilms, while none of these strains generated the biofilms in anaerobic conditions. Congo red binding results showed that both environmental cues and bacterial strains played a vital role in curli and cellulose production. Calcofluor binding and spectrophotometric results revealed that all these bacterial strains produced significantly lesser amounts of cellulose at 37°C, pH 8, and in high osmotic conditions as compared to the regular media, at 28°C, and pH 7. Metal biosorption was drastically reduced in these bacteria at 37°C than at 28°C. Only Vitreoscilla sp. ENSG301 and B. thuringiensis ENSW401 completely removed (100%) Cu and Ni at an initial concentration of 12.5 mg l–1, while all these bacteria totally removed (100%) Pb at concentrations of 12.5 and 25 mg l–1 at pH 7 and 28°C. At an initial concentration of 100 mg l–1, the removal of Cu (92.5 to 97.8%) and Pb (89.3 to 98.3%) was the highest at pH 6, while it was higher (84.7 to 93.9%) for Ni at pH 7. Fourier transform infrared spectroscopy results showed metal-unloaded biomass biofilms contained amino, hydroxyl, carboxyl, carbonyl, and phosphate groups. The peak positions of these groups were shifted responding to Cu, Ni, and Pb, suggesting biosorption of metals. Thus, these bacterial strains could be utilized to remove Cu, Ni, and Pb from aquatic environment.

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“…Agricultural drainage haves been reported to contain high levels of pesticides (Moore et al, 2008 andMoore et al, 2011). The microbial populations of the agricultural wastewater usually include some bacterial strains that can tolerate high concentrations of the toxic pesticides (Radwan et al, 2017). Moreover, these bacterial strains might have the capability to degrade and mineralize these compounds (Tyler et al, 2013).…”

Section: Isolation Of Atrazine Degrading Bacteriamentioning

confidence: 99%

Bioremediation of the nematicide oxamyl by Enterobacter ludwigii isolated from agricultural wastewater

Cited by 4 publications

References 0 publications

Metal-Adapted Bacteria Isolated From Wastewaters Produce Biofilms by Expressing Proteinaceous Curli Fimbriae and Cellulose Nanofibers

Metal-Adapted Bacteria Isolated From Wastewaters Produce Biofilms by Expressing Proteinaceous Curli Fimbriae and Cellulose Nanofibers

Biofilm Formation, Production of Matrix Compounds and Biosorption of Copper, Nickel and Lead by Different Bacterial Strains

Occurrence of Atrazine Biodegrading Bacterium "Ochrobactrum oryzae" In Agricultural Wastewater

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