Bioremediation of mortar made from Ordinary Portland Cement degraded by Thiobacillus thioparus using Bacillus flexus

Abstract

Cement is widely used as a construction material in the construction industry. However, there are challenges affecting its durability efficacy. Cement mortar/concrete is subject to degradation by aggressive ions such as sulphates and chlorides. Sulphates can be introduced into the concrete or mortar by Sulphur producing bacteria of the species Thiobacilli. Microbiologically induced CaCO 3 precipitation (MICP) has found its application in bioremediating cement based materials. It has been found to be environmental friendly. However, no work has been reported on bioremediation of biodegraded cement based materials. This paper presents findings of possible bioremediation of mortars after undergoing biodegradation. Bacillus flexus, a beneficial bacterium was used. The control mortars were prepared using Ordinary Portland Cement (OPC). The test mortars were prepared and cured in a solution of Thiobacillus thioparus, a Sulphur oxidizing bacteria, deleterious bacterium for 14, 28, 56 and 90 days. Compressive strength analysis was conducted on the 14 th , 28 th , 56 th and 90 th day of curing. Results showed that the lowest compressive strength was recorded on the 90 th day as 31.02 MPa. This was a 34.17 % loss in compressive strength. Another category of mortar cured in Thiobacillus thioparus for 28 days was bioremediated for 28 days using Bacillus flexus solution. Compressive strength and Scanning Electron Microscopy (SEM) analyses were then done. The results show a compressive strength of 45.83 MPa at the 56 th day. This represents a 99.91 % strength recovery from biodeterioration. The SEM analysis results revealed a denser material. This was due to massive precipitation of calcium carbonate in the mortar matrix and pores/voids for bioremediated mortars as opposed to the biodegraded mortars. The results further revealed reduced ettringite crystals on the bioremediated mortars. Bacillus flexus could perhaps be used in restoring lost compressive strength as well as in sealing voids in degraded concrete in sewer lines and other cement based materials. This could improve on its efficacy with minimal repair.

Complete Genome Sequence of Priestia flexa DMP08 Isolated from Kimchi, Traditional Korean Fermented Vegetables

“…The major research interest in P. flexa has concerned enzyme activity, such as of proteases and lipases [2, 3], as well as arsenic remediation [4]. Because of these enzymatic and remediative activities, P. flexa has been suggested to be a useful microorganism for detergents, food technology, pharmaceuticals, and biomedical science [5]. The β-amylase from P. flexa AE-BAF has been used as a food-grade enzyme [3].…”