Bioremediation of malachite green by cyanobacterium Synechococcus elongatus PCC 7942 engineered with a triphenylmethane reductase gene

Han, Sheng; Han, Wenbo; Sun, Yuankai; Dai, Meixue; Zhao, Guoyan

doi:10.1007/s00253-020-10438-w

Cited by 21 publications

(14 citation statements)

References 37 publications

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“…Similar, observations were also made for Citrobacter sp, which is previously known for MG degradation (Wang et al 2011(Wang et al , 2012. The engineered freshwater cyanobacteria Synchococcus containing triphenylmethane reductase gene 'katmr' was shown to produce a soluble recombinant protein that degraded malachite green into small molecules 4-methylaminobenzoic acid and 4-hydroxyl-aniline and that are nontoxic to wheat seeds (Han et al 2020). Huan-Mei et al 2011 opined that 'tmr' gene could have been horizontally transferred from Pseudomonas sp.…”

Section: Molecular Snapshot Of Bacterial Isolate For 'Lac' and 'Tmr' Genessupporting

confidence: 53%

Decolorization of Malachite green dye by Stenotrophomonas maltophilia a compost bacterium

et al. 2021

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Background The release of triphenylmethane dyes, like malachite green (MG) and crystal violet (CV), into the environment is a cause of concern due to its toxicity, mutagenicity and carcinogenicity. Result A bacterial strain that is capable of decolorizing both dyes was isolated from the composted neem oil-seed cake. The strain was characterized as Stenotrophomonas maltophilia based on the 16S rRNA gene sequence and designated as isolate TPMD-1. The kinetic study of the dye degradation revealed the efficiency of the above isolate to degrade MG. The effect of substrate concentration, pH, temperature, and agitation on the decolorization of MG by the isolate was also studied. The MG degradation rate was slightly more in neutral pH at 28–30 °C and 150 rpm. The UV-spectroscopy, HPLC, and FTIR analyses of the dye sample before and after bacterial treatment revealed the high ability of S. maltophilia TPDM-1 in the removal of MG from the media. The genetic snapshot of the isolate by PCR amplification and sequencing showed the presence of genes 'lac’ and ‘tmr’ that codes for laccase and triphenylmethane reductase. Conclusion This study presented the first report of Stenotrophomonas maltophilia in the degradation and detoxification of MG dye by oxidoreduction, which could be used for the bioremediation of aquatic environments contaminated by MG.

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Section: Molecular Snapshot Of Bacterial Isolate For 'Lac' and 'Tmr' Genessupporting

confidence: 53%

Decolorization of Malachite green dye by Stenotrophomonas maltophilia a compost bacterium

et al. 2021

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“…The mobile phase consisted of MeCN and H 2 O, both containing 0.1% v/v formic acid, and delivered using a binary gradient over 20 min followed by re-equilibration to the starting conditions. Based on previous literature studies − and to have enough residence time in the column, the flow rate was set at 0.4 mL min –1 and the injection volume was 5 μL. LC/MS analysis was conducted using both positive and negative ion detection, and elution of the analyte was monitored using the following MS conditions: nebulizer pressure 35 psi, gas flow rate 8 L/min, gas temperature 300 °C, capillary voltage 2500/–2500 V, fragmentor 150, and skimmer 65 V. The instrument was operated in the extended dynamic range mode with data collected in m / z range 50–1000. − …”

Section: Methodsmentioning

confidence: 99%

“…LC/MS analysis was conducted using positive mode electrospray ionization and elution of the analyte monitored in single ion monitoring (SIM) mode. Concentrations were determined by comparison to a set of calibration standards prepared in a blank solvent. − …”

Section: Methodsmentioning

confidence: 99%

Photocatalytic Degradation of 1,4-Dioxane and Malachite Green over Zinc Oxide/Cellulose Nanofiber Using UVA/B from Direct Sunlight and a Continuous Flow Reactor

et al. 2022

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Concerns over water contamination by biorecalcitrant, persistent organic pollutants have emphasized the need for effective, cheap, and scalable treatment methods. Photocatalysis offers the potential advantages of using air as the oxidant, ambient conditions for reaction, and sunlight as the source of energy; however, identifying a sustainable catalyst support and sunlight-active, earth-abundant photocatalysts is challenging. This study demonstrates for the first time the development of a ZnO/cellulose nanofiber (CNF) continuous flow photoreactor for fast and effective degradation of industrial pollutants 1,4-dioxane and malachite green and model pollutant methylene blue in water. The novel sun-flow reactor consists of open, interconnected parallel channels with a continuous flow of water containing the chosen pollutant circulated over the catalyst under ultraviolet (UV)A/B irradiation from direct sunlight. Mass spectrometry (MS), gas chromatography (GC)-MS, liquid chromatography (LC)-MS, and inductively coupled plasma (ICP)-MS analyses were used to investigate and quantify the extent of degradation and the byproducts formed in the process. Aqueous solutions of 10 ppm methylene blue and malachite green, as well as their reaction byproducts, were reduced below 0.1 ppm (the detection limit) with an energy per order (EE/O) figure of merit of 2.2 kWh/m3 per order. A 3.5 ppm aqueous solution of 1,4-dioxane was reduced to 0.034 ppm with an EE/O figure of merit of 1.6 kWh/m3 per order.

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“…Immobilization, co-contaminant influence on dye biodegradation, genetic modification of algae and cyanobacteria, graphene oxide addition and lipid production [137][138][139][140][141] Activated sludge, anaerobic sludge Granule formation (anaerobic core with aerobic shell), metagenomic analysis in anaerobic MBR, addition of resuscitation-promoting factors, integration of anaerobic and aerobic reactors, addition of halotolerant yeast and magnetic field [142][143][144][145][146][147][148][149][150] Biofilm Application of new biocarriers, co-substrate addition, kinetic analysis and process optimization in moving bed biofilm reactor, biomass acclimatization and optimization of anoxic/aerobic sequencing batch moving bed bioreactors [151][152][153][154][155] As seen from Table 2, there are numerous existing tried and tested methods to accomplish dye removal. Only in 2019, Deng et al [156] reviewed 42 papers on textile wastewater treatment technologies organized by physico-chemical, biological, and combined processes.…”

Section: Current Development Referencesmentioning

confidence: 99%

Recent Achievements in Dyes Removal Focused on Advanced Oxidation Processes Integrated with Biological Methods

2021

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In the last 3 years alone, over 10,000 publications have appeared on the topic of dye removal, including over 300 reviews. Thus, the topic is very relevant, although there are few articles on the practical applications on an industrial scale of the results obtained in research laboratories. Therefore, in this review, we focus on advanced oxidation methods integrated with biological methods, widely recognized as highly efficient treatments for recalcitrant wastewater, that have the best chance of industrial application. It is extremely important to know all the phenomena and mechanisms that occur during the process of removing dyestuffs and the products of their degradation from wastewater to prevent their penetration into drinking water sources. Therefore, particular attention is paid to understanding the mechanisms of both chemical and biological degradation of dyes, and the kinetics of these processes, which are important from a design point of view, as well as the performance and implementation of these operations on a larger scale.

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Bioremediation of malachite green by cyanobacterium Synechococcus elongatus PCC 7942 engineered with a triphenylmethane reductase gene

Cited by 21 publications

References 37 publications

Decolorization of Malachite green dye by Stenotrophomonas maltophilia a compost bacterium

Decolorization of Malachite green dye by Stenotrophomonas maltophilia a compost bacterium

Photocatalytic Degradation of 1,4-Dioxane and Malachite Green over Zinc Oxide/Cellulose Nanofiber Using UVA/B from Direct Sunlight and a Continuous Flow Reactor

Recent Achievements in Dyes Removal Focused on Advanced Oxidation Processes Integrated with Biological Methods

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