Bioremediation of heavy metals using biostimulation in laboratory bioreactor

Abstract

The present research study investigates bioremediation potential of biostimulated microbial culture isolated from heavy metals waste disposal contaminated site located at Bhayander (east), Mumbai, India. The physicochemical and microbial characterization including heavy metal contaminants have been studied at waste disposal site. The microorganisms adapted at heavy metal-contaminated environment were isolated, cultured, and biostimulated in minimal salt medium under aerobic conditions in a designed and developed laboratory bioreactor. Heavy metals such as Fe, Cu, and Cd at a selected concentration of 25, 50, and 100 μg/ml were taken in bioreactor wherein biostimulated microbial culture was added for bioremediation of heavy metals under aerobic conditions. The remediation of heavy metals was studied at an interval of 24 h for a period of 21 days. The biostimulated microbial consortium has been found effective for remediation of Cd, Cu, and Fe at higher concentration, i.e., 100 mg/l up to 98.5%, 99.6%, and 100%, respectively. Fe being a micronutrient was remediated completely compared to Cu and Cd. During the bioaccumulation of heavy metals by microorganisms, environmental parameters such as pH, total alkalinity, electronic conductivity, biological oxygen demand, chemical oxygen demand, etc. were monitored and assessed. The pilot scale study would be applicable to remediate heavy metals from waste disposal contaminated site to clean up the environment.

Alleviation of Heavy Metal Stress in Plants and Remediation of Soil by Rhizosphere Microorganisms

“…Use of genetically engineered microbes (GEM) well adapted to local conditions (soil and climatic) can also be done for efficient removal of HM from contaminated soils ( Das et al, 2016 ; Gupta and Singh, 2017 ). Biostimulation of local microbial population by adding nutrients can also be an approach to encourage remediation and detoxification of HM contaminated soils ( Fulekar et al, 2012 ). HMT microbes in consortium have recently been evaluated for their effectiveness in remediating HM from contaminated sites.…”

Biotechnological advances in bioremediation of heavy metals contaminated ecosystems: an overview with special reference to phytoremediation

“…The transgenic plants expressing merC-SYP121 were more resistant to cadmium than the wild type and accumulated significantly more cadmium, which indicated an ecologically compatible approach for the phytoremediation of cadmium pollution. Fulekar et al (2012) investigated bioremediation potential of biostimulated microbial culture isolated from heavy metals waste disposal contaminated site located at Bhayander, Mumbai, India. The microbial consortium has been found effective for remediation of Cd, Cu and Fe at higher concentration, that is, 100 mg/L up to 98.5, 99.6 and 100 %, respectively.…”

Removal of Heavy Metals by Indigenous Microorganisms and Identification of Gene Responsible for Remediation

“…The present research study deals with bioremediation of heavy metals with special reference to Cd, Cu and Fe using identified microorganisms from solid waste metal contaminated waste disposal site. The potential organism identified for each metal were sequenced and identified by BLAST techniques [1]. Bioremediation of metals (Fe, Cu and Cd) have been studied using microbial consortium as well as potential organism i.e.…”