Bioremediation of Contaminated Surface Water by ImmobilizedMicrococcus roseus

Abstract

The problems caused by contaminated surface water have gradually become more serious in recent years. Although various remediation technologies were investigated, unfortunately, no efficient method was developed. In this paper, a new bioremediation technology was studied using Micrococcus roseus, which was immobilized in porous spherical beads by an improved polyvinyl alcohol (PVA) - sodium alginate (SA) embedding method. The experimental results indicated that COD removal rate could reach 64.7 % within 72 hours when immobilized M. roseus beads were used, which was ten times as high as that of free cells. The optimum inoculation rate of immobilized M. roseus beads was 10 % (mass percent of the beads in water sample, g g(-1)). Suitable aeration was proved necessary to enhance the bioremediation process. The immobilized cells had an excellent tolerance to pH and temperature changes, and were also more resistant to heavy metal stress compared with free cells. The immobilized M. roseus beads had an excellent regeneration capacity and could be reused after 180-day continuous usage. The Scanning Electronic Microscope (SEM) analysis showed that the bead microstructure was suitable for M. roseus growth, however, some defect structures should still be improved.

Effect of Cell-to-matrix Ratio in Polyvinyl Alcohol Immobilized Pure and Mixed Cultures on Atrazine Degradation

“…Figure 5 presents the surface of the bead which had a complex mesh structure with pores larger than 2 µm. According to Li et al (2005), the beads with 2-µm pores should not have oxygen and substrate transfer problems.…”

Introductory Chapter: Alginates - A General Overview

“…Alginate is also used as an inoculant carrier for plant growth-promoting bacteria [31,32] and for bacteria with biodegradation ability [33].…”

Complete Genome Sequence of the Linear Plasmid pJD12 Hosted by Micrococcus sp. D12, Isolated from a High-Altitude Volcanic Lake in Argentina

“…Micrococci are widely distributed and have been isolated from diverse locations including extreme sites ( 1 – 4 ). Among members of the phylum Actinobacteria , the genus Micrococcus is of increasing biotechnological importance; Micrococcus species can be used for biodegradation and bioremediation processes ( 5 , 6 ), and they can be applied for producing useful compounds such as industrially relevant enzymes ( 7 , 8 ) and long-chained alkenes for the environmentally friendly substitution of fossil fuels ( 9 ). Micrococcus sp.…”