Bioremediation of Chlorobenzoic Acids

Vrchotová, Blanka; Macková, Martina; Macek, Tomáš; KateřinaDemnerová,

doi:10.5772/56394

Cited by 4 publications

(10 citation statements)

References 95 publications

(97 reference statements)

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“…The HPLC results confirmed that these results were reliable as the screening for the compound following extraction of the same aliquot product of the experiment did not show the presence of the compound. The degradation of PCBs by Pseudomonas strains from Notwane Sewage Treatment Plant has not been reported before and thus these results suggest that strains from Gaborone wastewater have the ability to degrade recalcitrant compounds as reported from other studies in different areas [7,25,27].…”

Section: Resultssupporting

confidence: 65%

“…The shifts are an indication of the presence of initial ring oxidation metabolites and ring fission metabolites [25,27] PCBs first get degraded into chlorobenzoates [27] that have been found to have λ max ranging from 244-270nm in the C-band when dissolved in water [28], a range that was observed in the results obtained after 24 hours of incubation of treated wastewater with bacterial isolate MD2 used in this study and PCBs aroclor 1260 (Figure 1) which proves that the bacteria was able to breakdown the compound. The product chlorobenzoate is biodegraded into benzoate and eventually pyruvate and acetyladehyde which are essential in the tricarboxylic cycle (TCA) [25,27].…”

Section: Resultsmentioning

confidence: 99%

“…The product chlorobenzoate is biodegraded into benzoate and eventually pyruvate and acetyladehyde which are essential in the tricarboxylic cycle (TCA) [25,27].…”

Section: Resultsmentioning

confidence: 99%

“…These characteristics and the underlying molecular mechanisms thereof, are of great importance in the development of enhanced wastewater recycling methods [13,24]. In degradation of PCBs, chlorobenzoic acids (CBAs) are formed which are biodegradable by most bacteria [25].…”

Section: Introductionmentioning

confidence: 99%

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Biodegradation of Polychlorinated Biphenyls (PCBs), Aroclor 1260, in Wastewater by Isolate MD2 (Pseudomonas aeruginosa) from Wastewater from Notwane Sewage Treatment Plant in Gaborone, Botswana

2014

J Bioremed Biodeg

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Notwane Sewage Treatment Plant is situated on the eastern part of Gaborone emptying its effluent into Notwane River which flows along the periphery of Gaborone. The wastewater treatment plant receives wastewater from most major industries, and most of the residential waste water for the city of Gaborone. The wastewater is not recycled despite the great shortage of water in Gaborone and surrounding villages and the drying up of Gaborone dam.Wastewater samples from the treatment plant was collected and taken to Department of Biological Sciences in Mafikeng for analysis of PCB (Aroclor 1260) degradation by bacteria. Biodegradation of polychlorinated biphenyls using MD2 was conducted using Aroclor 1260 added to wastewater obtained from Notwane Sewage Treatment Plant by measuring shift in the wavemax (λ max ) nm using Cary 300 UV-visible spectrophotometer, for a period of 96 hours. The presence/absence of the compounds was checked using High Performance Liquid Chromatography (HPLC) UFLC Shimadzu using florescence detector pump RF-20A and system gold column C18 (CTO-20A) after 96 hours.The bacterial strain MD2 was found to have completely degraded the Aroclor1260 in wastewater after 96 hours. A shift in maximum wavelength was observed to be from 224 nm to 270 nm, an indication that Aroclor 1260 was degraded forming a chlorobenzoate derivative. This renders the wastewater free from polychlorinated biphenyls thus can be safely recycled.

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Section: Resultssupporting

confidence: 65%

Section: Resultsmentioning

confidence: 99%

“…The product chlorobenzoate is biodegraded into benzoate and eventually pyruvate and acetyladehyde which are essential in the tricarboxylic cycle (TCA) [25,27].…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biodegradation of Polychlorinated Biphenyls (PCBs), Aroclor 1260, in Wastewater by Isolate MD2 (Pseudomonas aeruginosa) from Wastewater from Notwane Sewage Treatment Plant in Gaborone, Botswana

2014

J Bioremed Biodeg

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“…CBAs have also been found to be generated as intermediates in the process of the degradation of the same herbicides. To be more specific, 2,6-CBA is produced as an intermediary by microorganisms during the breakdown of dichlobenil (Holtze et al, 2008), 2,5-CBA is produced during the degradation of chlorambene (Kamei et al, 1995;Dietrich et al, 2006), and 4-CBA is the ultimate product of the degradation of the insecticide DDT (Vrchotová et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of 2- Chlorobenzoic Acid and its other substitutes

AL-Quraishi¹

2022

J. basic appl. Res. Biomed.

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Because of the widespread use of these halogenated organic compounds in agriculture and industry, considerable quantities of these compounds' byproducts are discharged into the environment. These chemicals are known as halogenated pesticides. There is one group that, based on the chemical structures of these compounds, is thought to be the most tenacious and poisonous of all the groups. This group is known as the group. A. hydrophila, which was shown to be capable of utilising chlorobenzoate chemicals as a carbon and energy source, was isolated from wastewater treatment plant effluent in Petra, Jordan. Different biodegradation rates (4-chlorobenzoic acid, 5 M/hr; 3,4-dichlorobenzoic acid, 15.5 M/hr; 2-chlorobenzoic acid, 41 M/hr; 3-chlorobenzoic acid, 65 M/hr) were used to achieve these capacities. The release of chloride, the disappearance of the substrate, and finally the development of bacterial cells on these substrates were used to monitor the ability to degrade. Analyses of the ortho or meta ring-cleavage of these aromatic compounds were conducted on A. hydrophila dioxygenases that were physiologically activated by chlorobenzoic acid compounds. The fact that only one 2-dioxygenase activity was found suggests that the ortho route is used for the cleavage. The optimal conditions for 2-CBA chemical breakdown were 3 mM substrate concentration, 25 oC, pH 7, and 200 l inoculum size. Differently from how they affected chloride and cell mass synthesis, the carbon sources had an impact on the breakdown of 2-CBA. Utilized nitrogen sources decreased 2-CBA's ability to degrade and its ability to release chlorine. However, the biodegradation between the 40 and 80 hours was slightly enhanced by the nitrogen source L-proline.

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